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Introduction: Pain is one of the main complaints of many postoperative patients. Narcotic and NSAID drugs are used to control postoperative pain.
Recent studies suggest different results of pain relief by NSAID group. We compared analgesic effects of intramucular Pethedine to Didofenac Sodium and Indomethacin suppositories due to inguinal hernioplasty.
Materials and Methods: This study was a clinical trial study over 55 patients of 17-60 years old who underWent unilateral inguinal hernioplasty.
These patients divided into 3 groups. The first group included 17 patients who received 100 mg Indomethacin suppository every 8 hours to relief postoperative pain. The second group of 18 patients who received 100 mg Diclofenac Sodium suppository every 8 hours and the third group induded 20 patients who received 0.5 mg/kg body weight Pethedine intramuscularly every 8 hours. The first dose of each drug started 2 hours after termination of operation. Tue sevrity of pain was checked by visual analogue scale method every 2 hours for 24 hours. Mean pain severity recoded and compared in 6 hours intervals.
Results: The averages of pain severity in the first 24 hours were 23±12 for Indomethacin, 31±9 for Pthedine and 27±12 hours for Diclofenac Sodium groups. There was no significant difference in whole 24 hours.
Conclusion: We concluded that Indomethacin and Diclofenac Sodium suppositories are good substitutes for intramuscular Pethedine to relief postoperation pain during the first postoperation day.

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Introduction: The two therapeutical methods, ambulatory phelebectomy and stripping, used in the treatment of venous varices seem to have different duration of admission and complications. In this study the variables which seemed to be different between these two approaches have been compared with each other.
Materials and methods: In an analytic cross-sectional investigation, 43 patients were operated for venous varices with stripping and ambulatory phlebectomy were studied. Gender, age, type of operation, post- operative complications (hematoma, pain and paresthesia), duration of admission and the duration of operation were recorded for all subjects.
Results: Out of 43 patients, 19 were operated by ambulatory phlebectomy and the other by stripping methods. There was no meaningful difference in the groups with respect to the sex. There was a meaningful difference between both groups with respect to hemetoma (5.3% versus 0%).The average duration of admission and operation in the stripping was virtually more than the ambulatory phlebectomy.
Discussion: The duration of operation, hematoma, pain and paresthesia was proportionally more in the stripping procedure than the ambulatory phlebectomy. Thus, ambulatory phlebectomy can be more helpful in the treatment of venous of limbs.

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Introduction: Peptic ulcer is a mucosal lesion of stomach or deodenum in which acid and pepsin play major pathogenic roles. In general population, Helicobacter pylori (HP) plays an important role in peptic ulcer. Peptic ulcer is more common in chronic renal failure (CRF) but its etiology is unknown. Materials and methods: In a descriptive cross-sectional study, the prevalence of peptic ulcer in CRF patients was studied and its relationship with HP was evaluated. All patients were visited in Tajrish shohada hospital, Tehran. CRF was defined as a serum ceratinin concentration equal or more than 2 mg/dl at least for 3 months. Patients who stressful conditions, or were recieved immunosuppressive or NASID drugs, or drugs for eradication of HP were excluded. Upper GI endoscopy was performed for all patients by gastroentrologists to find out peptic ulcer. Biopsy was taken for HP identification by rapid ureas test. Results: The mean age of participants was 56 year and 51% of them had GI symptoms. Nineteen of 98 patients with CRF (19%) had peptic ulcer. That 5 of them were positive HP. HP was identified in 45 (46%) of patients. Conclusion: This study showed that HP is not more prevalent in CRF patients compared to general population. Additionally, peptic ulcer was more common in male patients with CRF than non-CRF subjects.

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Introduction: Brucellosis is one of the most important zoonotic diseases that causes miscarriage and infertility in animals and causes human fever. The use of the common SS9 strain of Brucella abortus has several side effects for livestock. Brucella P39 protein is one of the plasma peripheral space proteins that is considered as one of the important immunogenic indicators. With the production of the new protein combination of P39, more studies can be done on the ability of this protein to stimulate immune responses against Brucella. Therefore, in this research, the production and purification of this protein in Escherichia coli bacteria has been done as a new compound.
method: In this experimental study, using the polymerase chain reaction, the P39 gene was propagated by the bacterium Brucella abortus. After purifying the P39 gene, it was cloned into plasmid carriers pSK+ and pGEX4T1. Therefore, pSK+-P39 and pGEX4T1-P39 structures were prepared. To produce the recombinant protein P39, the plasmid structure pGEX4T1-P39 first entered the Escherichia coli bacterium BL21. The protein was then produced by IPTG by induction of pGEX4T1-P39 plasmid. The resulting protein was purified using the orderly purification protein glutathione S-transferase. The amount of purified protein was measured using the Brad Ford method.
Results: The nucleotide sequence of the gene propagated by the cloned PCR in the plasmid carrier  pSK+ was exactly the same as the P39 gene of Brucella abortus. Production of P39 protein was performed by induction of pGEX4T1-P39 plasmid. The purified protein content was 200 micrograms per milliliter.
Conclusion: The production of the new protein P39 compound Brucella Abortus, which is unstable in the cytoplasm of the Escherichia coli bacterium, is possible using carriers with additive proteins such as pGEX4T1 in the host of Escherichia coli strain BL21.

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Introduction: Hidden hepatitis B is a condition in which the surface antigen of hepatitis B in the patient's serum is negative but the DNA of the hepatitis B virus is detected in the serum or liver tissue. In this study, the prevalence of latent hepatitis B in chronic hepatitis C patients and their biochemical and histological changes were investigated.
method: In this descriptive study, target sampling was performed so that 27 chronic hepatitis C patients whose HBsAg was negative and during 2001 and 2002 to two hepatitis centers of Tehran and Research Center for Gastrointestinal and Liver Diseases of Shahid University of Medical Sciences Beheshti came in and underwent liver sampling. On the hepatic paraffinic block of these patients, polymerase chain reaction tests were performed for the presence of HBVDNA, as well as immunohistochemical tests for the presence and detection of HBsAg and central hepatitis B antigen.
Results: Of the 27 PCR samples examined, patients reported positive HBVDNA in 5 cases (19%). In all of these patients, IHC tests were reported to be negative for HBsAg and HBcAg. Histological changes of cirrhosis and irreversible cirrhosis symptoms were seen only in the HBVDNA group.
Conclusion: The prevalence of latent hepatitis B is relatively high in patients with hepatitis C. In these patients, latent hepatitis B can exacerbate liver damage and accelerate the progression of cirrhosis.

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Introduction: Tension  headache  is  the  most  common  type  of  headache. Tension  headache is  mostly  caused  by  stress, anxiety  and  depression.  Treatment  is  by  pharmacological  and  nonpharmacological  methods.  Nowdays  nonpharmacological  methods  are  used  increasingly. This  is  a  response  to  the  over  use  of  drugs, side  effects  and  cost.  The  purpose  of  chronic  tension  headache.
Materials  and  Methods: This  research  is  a  clinical  trial  study. 25  women  suffered  from  chronic  tension  headache  were  treated  by  massage  during  5  weeks. Samples  were  asked  to  record  headache  indexes  in  a  checklist  four  times  a  day  (before  breakfast, before launch , before  dinner  and  before  sleep)  through  these  5  weeks. Data  from  the  first  and  fifth  weeks  were  recorded  as  pre  and  post intervention  data and  data  from  the  second, third  and  fourth  weeks  of  investigations  were  recorded  as  the  data  during  intervention.  During  the  intervention  samples  were  treated  twice  a  week  for  20  minutes  each  time  with  the  friction  massage  on  posterior  parts  of  the  neck  and  shoulders.  Headache    indexes  included: intensity, frequency  and  duration  of  headache, headache  intensity  was  recorded  using  11  points  criteria. Headache  frequency  was  measured  by  calculating  the  days  of  headache  in  a  week  and  the  duration  of  the  headache  was  calculated  by  dividing  sum  of  headache  hours  in  a  week  to  the  frequency  of  attacks. Data  was  analyzed  by  analysis  of  variance  and  Tukey  test.
Results: Results  showed  the  positive  effect  of  massage  in  which  headache  indexes  showed  a  meaningful  difference  in  three  stages  of  pre, during  and  post  intervention (p<0.01).
The  recovery  rates for  each  index  were  52%, 28%  and  57%  respectively.
Conclusion: Massage  therapy  resulted  in  the  reduction  of  intensity, frequency  and  duration  of  tension  headache  and  can  be  useful  in  treatment  of  tension  headache .

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Introduction: Oxidative  stress is  one  of  the  factors  producing  poisoning  during  chronic  exposure  to  organophpsphporous  poisons.  In  this  research  we  studied  oxidative  stress  status  and  acetycholinesterase  in  pesticide  manufacturing  workers.
Materials  and  Methods: This  is  a  cross-sectional  analytical  study  in  which  45  organophopsphorous (OP)  formulating  pesticide  workers  with  a  minimum  work  history  of  1  year  in  the  age  range  of  23-35  were  studied. Controls  were  age–matched  workers  of  a  food-making  factory.  They  were  evaluated  for  oxidative  stress  markers, including  Tthiobarbituric  acid-reactive  substances (TBARS)  indicator  of  lipid  peroxidation (LPO), ferric  reducing  ability  of  plasma (FRAP)  indicator  of  total  antioxidant  capacity, total  Thiol (SH)  groups  and Gammaglutamyl  transpeptidase (GGT)  levels  in  blood  and  ACHE  activity  in  erythrocytes.
Results: The  results  showed  marked  inhibition  of ACHE, increased  TBARS, decreased  FRAP  and  decreased  Thiol  group  level  in  workers.  The  reduction  in  activity  of  ACHE  correlated  well  with  increased  TBARS  and  decreased  FRAP  in  OP  formualtors.
Conclusion: It  is  concluded  that  Op-formulating  workers  are  exposed  to  more  oxidative  stress. The  measurement  of  erythrocyte  ACHE  activity  in  pesticide  workers  who  formulate  Ops  can  be  a  good  monitoring  factor  and  is  recommended  to  be  performed  in  a  regular  manner.
 

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Introduction: Acute  renal  failure (ARF)  is  one  of  the  relatively  incident  complications  in  surgical  emergency  wards  and  is  defined  as  increasing  serum urea  and  creatinine   and  decreasing  GRF. In  this  study  we  investigated  acute  renal  failure  incidence  in  patients  admitted  to  emergency  surgical  ward.
Materials  and  Methods: This  is  a  cross  sectional  analytical  study  in  which  all patients  with  primary  diagnosis  of  emergency  surgery  (traumatic  or  non traumatic) admitted  in  emergency  surgery  ward  were  selected. After  history  taking  and  physical  assessment  patients  having  history  of  kidney  disease  were  excluded. Urinalysis, BUN  and  creatinine  tests  were  performed  for  all  samples. Data  was  analyzed  by  SPSS  software.
Results: In  this  study  2100  patients  (1280  male)  were  assessed  during  a  10  month  period. 5.66%  of  samples  had  some  degree  of  ARF. 1136  patients  undergone surgery  due  to  accidents  and  trauma  (72%  multiple  trauma  and  28%  single  trauma) and  964  patients  due  to  nontraumatic  emergencies.  ARF  was  seen  in 6.84%  of  the  first  and  4.25%  of  the  second  group. ARF incidence  in  male  and  female  was  5.6%  and  5.7%  respectively.
 ‍Conclusion: The overall  incidence  of  ARF  in  this  study  was  not  so  different  from  developed  countries.   ARF  was  significantly  more  incident  in  traumatic (especially  multiple  trauma)  and  old  aged  patients.  This  factors  must  be  considered  as  ARF  risk  factors  in  emergency  wards.

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Introduction: Different groups of drugs are used for prevention of migraine headaches. Howevere, there are controversy about the afficacy of these drugs. Furtheremore, the priority of one to other group is not clear. This study was designed therefore, to compare the effects of Propranolol and Nimodipine on sevierity, duration and frequency of migraine headache.
Materials and Methods: In a clinical trial study, 102 patients with migraine headache refered to neurology clinic were chosen. Patients were devided into two drug groups. Group one received 40 mg Propranolol per day and group two received 30 mg Nimodipine. Data was collected by a questionnaire. Patients were asked to record the severity, duration and frequency of their migraine attacks. Data was analysed using t-test. p<0.05 was considered significant.
Results: The duration and severity of migraine attacks in Propranolol group were less compared to Nimodipine group (p<0.05). However, there was no statistical differences in the frequency of migraine of these two groups.
Conclusion: Propranolol seems to be more effective than Nimodipine in prevention of migraine headaches

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Introduction: Hyperlipidemia is one of the main known risk factors for coronary artery disease. Regarding the undesirable consequences of hyperlipidemia and its long-term treatment, it is important to use drugs with lesser complications during the long period of treatment.
Materials and Methods: This study was a double-blind randomized clinical trail that carried out on 65 patients with hyperlipidemia. Patients were divided into two groups. Case groups took dill pearl, and control group took placebo pearl for one month. Serum lipids of both groups were measured before and one month after interference. Data was analysed with t-test and p<0.05 was considered significant.
Results: In this study , differences between mean level of cholesterol, triglyseride, HDL and LDL in case group before and after interference were statistically significant (P<0/05) but in control group only difference between HDL before and after interference was significant .
Conclusion: Regarding the result of the study dill pearl can be a useful drug for treatment of hyperlipidemia.

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Introduction: Cystic hygroma is a disorder in lymphatic vessel formation that involves the adjacent organs and can affect them due to its fast growing nature . The main treatment for cystic hygroma is surgical intervention that can have many complications after surgery. Treatments other than surgical interventions is the use of sclerosing agents. The goal of this study is to analyze the effect of Beliomycin in treating cystic hygroma . Materials and Methods: This quasi experimental study was carried out clinically at st-al Zahra and Kashanie hospitals of Isfahan from 1372 to 1383. A checklist including age, sex, cyst location, cystic hygroma volume before and after Beliomycin injection and/or surgery, was completed for each patient. Data was analyzed by T and paired T-Tests. p<0.05 was considered meaningful. Results:The study was carried out on 72 patients. 24 patients were treated with Beliomycin and 48 patient by surgery. Patients age was below 1 month up to 18 years old . Cystic hygroma was mainly located at neck region(46.8%). The average size of cystic hygroma was 103.9±29.66cm3 before Beliomycin injection and 34.91 16.19cm3 after that (p=0.004). and 43.511±7.81cm3 before surgery and 1.39±1.39 cm3 after that(p=0.006). The rate of recurrence of lymphingoma in the surgery treated group was 25.1% and in the group treated with Beliomycin was 29.2% with no significant difference. Conclusion: Regarding the considerable decrease in cyst volume after Beliomycin injection and it,s low complication compared to surgery, this method can be used as a primary treatment or supplementary to surgery.

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Introduction: In many studies, immunogenicity of Brucella proteins such as P39 in animals is investigated. In this study, we evaluated antigenicity of recombinant P39 from Brucella abortus in patients with Brucellosis. Materials and Methods: In this experimental study, at first recombinant P39 was produced in Escherichia coli. Sera reactivity of six infected individuals against the recombinant P39 protein was analysed by Western Blot. Results: Data indicated that P39 protein from Brucella abortus was recognized by patients, sera antibodies. Conclusion: Our data showed that recombinant P39 protein can be detected as an antigen by sera in infected human. Therefore, recombinant P39 have same epitopes with natural form of this antigen.

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Introduction: The reason of infertility can have a direct influence on the plan and outcome of management. In this paper we intend to show the effect of tuberculosis (TB) history on female infertility among infertile couples attending to Rooyan infertility management center. Materials and methods: In this case-control study our cases were those who were diagnosed as infertile female and controls were those women whose husbands were infertile due to some male factor. We used logestic regression for analyzing the association of history of Tuberculosis and female infertility with attributable risk estimation. Results: 308 cases were compared to 314 controls. Considering the odds ratio and its 95% confidence interval, there was a significant difference between the history of TB and infertility (OR=4.7, 95% CI: 1.01-29.91). The attributable risk of TB for female infertility was 0.023±0.01 (which is significant at 5% level). Conclusion: These figures show that at least 2% of female infertility can be prevented by prevention and proper treatment of tuberculosis.

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Introduction: Sensory stimulation of comatos patients is an important nursing intervention for increasing their LOC and tactile stimulation especially massage is one of the most important stimulations. The nurse as a person, who is responsible for this, must know the effects of this intervention on patients. But there are some conflicts about this intervention's effects especially on vital signs (pulse, blood pressure and respiration). This study was done to determine the effect of massage on comatos patient's vital signs who were hospitalized in intensive care unit. Materials and Methods: This study is a clinical trial. Samples were consisted of 100 comatos patients, divided into two equal groups randomly. The two groups were matched in age, geneder and glascow coma scale. Samples in experimental group received 20 minutes massage on their back, feet and hands in three continuous days. Vital signs in experimental group were measured before and immediately after intervention and vital signs in control group were measured at the same time with experimental group. A check list used was used for collecting data. Data was analyzed using descriptive and analytic statistics (T, paired T and Chi-square tests). Results: Findings showed that massage decreased vital signs significantly as followed: pulse rate and diastolic blood pressure in each three days (p<0.05), systolic blood pressure in first and third days (p<0.05), respiration rate in second day (p<0.05). Conclusion: This study showed that massage can decrease comatos patients' vital signs which can be the indicator of their relaxation and decreasing stress.

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Introduction: There is little information about essence and combination of ductuli efferentes secretions. Glycoconjugates importance in sperm production and maturation has been confirmed in previous studies therefore this study is done in order to recognitize Glycoconjugates in ductuli efferentes epithelium and determination of their distribution pattern by means of lectin histochemistry. Materials and Methods: In this descriptive study tissue species were obtained from 30 adult male BALB/c mice. After fixation and routine laboratory processes, 5 μm sections were prepared from paraffin blocks. These slides were exposed to different lectins by means of lectin histochemistry. Reaction intensity in different cells was investigated by light microscope and graded according to the previous related studies. Then results were compared with each other by Kruskal-Wallis and Dunn statistical tests. Results: The mean of reaction intensity in ductuli efferentes epithelium in reply to different lectins, showed significant statistical difference (p<0.005). The most intense reaction was seen in response to WGA (Wheat Germ Agglutinin) lectin and after that to SBA (Soybean Agglutinin), VVA (Vicia Villosa Agglutinin) and PNA (Peanut Agglutinin) respectively. But the staining was negative with GSA-I (Griffonia Simplicifolia Agglutinin-I) lectin. Conclusion: Results indicate that the cells of ductuli efferentes epithelium in mouse are involved in synthesis and secretion of sperm maturation related glycoconjugates and produce a variety of these components in various amounts. There is a large amount of components, containing terminal sugars such as Sialic acid,Galactose-N-Acetylgalactosamine and N-Acetylgalactosamine in ductuli efferentes cells. The lack of Galactose in this epithelium shows that, it hasn’t a role in sperm maturation.

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Introduction: Plantar fasciitis is one of the common complications in adults. It can be treated by surgical and nonsurgical interventions. Nonsurgical treatments (functional foot orthosis, heel pad, night splint and wedge) are emphasized as the main option in management of plantar fasciitis. Wedge can be used effectively because of its low weight, low price, easy making and availability to the patient. In this study the effect of medial heel wedge on managing pain in patients suffering from plantar fasciitis was examined. Materials and Methods: In this before and after clinical trial, 15 patients with plantar fasciitis including 6 male and 9 female with 18 involved extremities were selected by non probability sampling.Variables including pain, ADL, sport and recreational activity, quality of life, stride length and step length were measured in three stages: once before intervention and then two and four weeks after intervension. For evaluating the variables, FAOS questionnaire was used. Data was analyzed using ANOVA. Results: The improvement of variables such as pain, ADL, quality of life, sport and recreational activity, step length and stride length was significant (p=0.05). Conclusion: Medial heel wedge reduced pain by shifing the center of force into lateral border of heel in addition to reduce the tension of plantar fascia.

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Introduction: Using one subcutaneous infiltration of Bopivacaine can cause a good pain relief in pediatric surgery, so the child may no longer need rectal Acetaminophen after surgery. In this study, we compared the efficacy of the two drugs. Materials and Methods: This was a clinical trial, performed on 141 pediatric patients between 6 months to 3 years old with unilateral or bilateral inguinal hernia or undescending testis. Patients were divided randomly into two groups. The first group received infiltration of Bopivacaine preoperatively and the second received the routine rectal Acetaminophen after surgery. In both groups pain score was measured with FPRS score 3,6,12 and 24 hours after surgery. After collecting data they were analyzed with T-student and Friedman tests. P<0/05 was considered significant. Results: Global FPRS pain score was lower in the group treated with Bupivacaine infiltration on 3 and 6 hours after surgery (p<0/05), but in 12 and 24 hours after surgery no significant difference was seen in the two groups. Conclusion: This study showed that, infiltration of Bupivacaine reduces pain better than Acetaminophen, especially during the first 6 hours after surgery. So because of better pain control and also single administration, we suggest using infiltralition of Bupivacaine preoperatively.