Showing 4 results for رزماری
Rouhollah Hemmati Bushehri, Mahmoud Reza Jaafari, Ghasem Mosayebi, Ali Ghazavi, Ali Ganji,
Volume 27, Issue 6 (1-2025)
Abstract
Introduction: Nowadays, plant-derived compound is widely used in medical treatment as complementary therapies. Rosemary is one of these plants that have anti-angiogenic and anti-tumor effects. However, low solubility and low bioavailability lead to limited use of this compound. To overcome this limitation, the use of liposomes is beneficial. So, this study aimed to prepare liposomes containing rosemary alcoholic extract to obtain the best formulation with best properties to use in the antitumor study.
Methods: Liposomes were prepared by Thin-Film-Hydration method in different ratios of rosemary extract, hydrogenated-soybean-phosphatidylcholine (HSPC), Cholesterol, and mPEG2000 (F1-F6). The characterization of nanoliposomes includes size, PDI (Poly dispersity index), zeta potential, encapsulation efficiency, and release rate was measured.
Results: The results showed that the average particle size and zeta potential were 113.4 nm and -10.8 mV, respectively. Encapsulation efficiency showed a range of 60- 90%. The average release rate of the extract was about 60%. Also, the results showed that the F1 formulation was transparent, had appropriate properties, and did not precipitate. F2 formulation was not transparent. F3-F4 formulations were precipitated, and F5-F6 formulations did not achieve the appropriate size.
Conclusions: This study proved that F1 formulation in a ratio of 55:30:5 HSPC/chol/mPEG2000 is the best formulation for the preparation of nanoliposomes containing rosemary alcoholic extract to achieve the best properties for use in cancer studies.
Mojgan Mohammadifar, Fereshteh Bahmani, Alireza Abed, Gholamali Hamidi, Mohammad Hossein Aarabi, Sayyed Alireza Talaei,
Volume 28, Issue 4 (10-2025)
Abstract
Introduction: The analgesic effects of rosemary and peppermint essential oils have been shown in many studies. Low solubility of essential oils in water and low bioavailability limit their consumption, and nanoparticles can help solve these limitations. This study aimed to investigate the analgesic effect of a nanoemulsion containing rosemary and peppermint essential oils in a rat model of neuropathic pain.
Methods: Forty male rats were assigned to four groups: control (CON), neuropathic pain received normal saline (NPS), nanoemulsion (NPN), or gabapentin (NPG). Neuropathic pain was induced using the CCI model. Animals received a nanoemulsion containing 10% rosemary extract and peppermint essential oil via gavage for 21 days. Behavioral tests (Thermal Hyperalgesia, Thermal Allodynia, and Mechanical Allodynia) were done on days 1, 4, 7, 14, and 21 of the study. Biochemical tests were also done on the sciatic nerve of animals at the end of the study.
Results: The NPS rats had a significantly increased pain threshold compared to the control group (P < 0.001). Furthermore, the analgesic potential of nanoemulsion containing rosemary and peppermint essential oils was seen in allodynia and hyperalgesia tests. Biochemical analyses showed that CCI reduced total antioxidant capacity, superoxide dismutase, and glutathione peroxidase activity, and also increased MDA levels in the rats' sciatic nerve (P < 0.001 for all comparisons). Administering nanoemulsion containing rosemary and peppermint essential oils reversed all the above-mentioned changes in the biochemical parameters (P < 0.001).
Conclusions: Our findings revealed that treating rats with a nanoemulsion containing rosemary and peppermint essential oils can reduce neuropathic pain symptoms by balancing their sciatic nerve's oxidant and antioxidant systems.
