Showing 36 results for Breast
Ahmad Hamta, Milad Pezeshki, Jamshid Ansari,
Volume 20, Issue 12 (3-2018)
Abstract
Abstract
Background: Biological and epidemiological data suggest that damage induced by endogenous and exogenous factors affects the integrity and stability of DNA and associated with susceptibility to breast cancer. The XRCC3 protein participates in DNA double-strand breaks and recombination repair. The aim of the present study was to evaluate associations between the risk of breast cancer and Thr241Met polymorphism in the XRCC3 gene.
Materials and Methods: In this study, the effects of Thr241Met polymorphism of the XRCC3 gene and the risk of breast cancer in a population-based case-control study inclusive 80 patients and 80 healthy individuals of women in Markazi province were evaluated. Genomic DNA was extracted from blood samples using the kit procedure. The genotypes of samples were determined by PCR-RFLP technique. Statistical analysis was done using SPSS software (estimation of χ2 and p-value) and the final results were determined.
Results: Statistically significant difference was observed between the two groups of patients and controls for three genotypes of the site rs861539 (p= 0.000). Genotype CT (p= 0.000, OR=2.352, CI= 95%; 2.431 - 39.948) and TT (p = 0.003, OR= 2.352, CI=95%; 0.611 - 9.049) significant associations were showed with risk of breast cancer. Instead, the genotype CC (p= 0.000) showed a protective role against susceptibility to breast cancer.
Conclusion: This study identified that there is significant association between Thr241Met polymorphisms of the XRCC3 and the risk of susceptibility to breast cancer, which is in accordance to some of researchers' studies.
Ali Arash Anoushirvani, Azam Ahmadi, Reza Aghabozorgi, Sara Khalili, Maryam Sahraei, Taha Fereydouni, Zoha Khademi,
Volume 21, Issue 2 (5-2018)
Abstract
Abstract
Background: Breast cancer is the most common cancer in women. It has been proven the association of cause of this disease with changes in several genes. One of the pathways associated with breast cancer is the folate reuptake pathway. The key enzyme of this pathway is coded by the TYMS gene. MicroRNAs control the expression of genes by binding to their regulatory regions. In this study, we evaluated changes in the regulatory region of TYMS gene with demographic characteristics (including the grade of cancer and metastasis) in breast cancer patients.
Materials and Methods: In this study, the regulatory region of TYMS gene was investigated using related bioinformatics software. After collecting cancerous samples and DNA extraction from blood samples of normal and patients, change in the miRNA binding region by digestion with NlaIII enzyme was assayed.
Results: Bioinformatics studies showed that the restriction site of some of the endonuclease enzymes in the 3'-UTR of the TYMS gene is related to the binding region of miRNAs, including Hsa-miR-433-3p. The results indicated the correctness of the genomic purification process, the PCR and enzymatic digestion reaction. In this study, in the regulatory region, CC homozygote, AC heterozygote and AA mutant homozygote variant had differences with control group (OR: 1.3465, %95 CI: 0.7275 to 2.4923, p<0.05). Also, the association of AA genotypes with metastasis and high grade of the patients was confirmed statistically.
Conclusion: Studies have shown that some of polymorphisms in the key genes involved in cancer are directly related to their diagnosis and treatment process, and given the importance of timely diagnosis of cancer, the achievement of diagnostic biomarkers in breast cancer in the early stages will be important. Probably, the nucleotide change at the site of the microRNA binding site could be used as a diagnostic biomarker for degree of tumor progression.
Fatholah Mohaghegh , Mehran Mohseni, Nasrin Robatmili, Mohamad Reza Bayatiani , Fatemeh Seif, Nayyer Sadat Mostafavi,
Volume 21, Issue 6 (12-2018)
Abstract
Background and Aim: Radiation therapy is the destruction of cancer cells that in all patients with breast cancer reduces tumor recurrence, relieves pain in local tumors and metastases. There are different treatment methods around the world such as electron, photon alone or a combination of both types of fields.
Materials and Methods: In this study, photon therapy (PT) and mixed photon-electron therapy (MPET) were used to treat malignancies of the supraclavicular lymph nodes. 30 patients with right-sided breast cancer with local lymph node metastasis were recruited. The ISOgray software was utilized to collect data about treatment planning methods with PT and MPET.
Findings: The maximum and mean delivered doses of radiation to the supraclavicular region were 52.08±1.64, 42.59±0.51 Gy and 54.24±1.64, 43.67±0.43 Gy in the PT and MPET methods, respectively. The mean irradiated volumes of supraclavicular fossa that received 90% of the radiation dose were 59.74±1.94% and 70.26±0.94% in the PT and MPET methods, respectively (p=0.004). The maximum doses delivered to the spine were 14.66±1.9 Gy and 10.22±0.92 Gy and the thyroid were 42.62±3.1 Gy and 37.67±5.02 Gy in the PT and MPET methods, respectively.
Conclusion: The maximum doses delivered to the spine and thyroid significantly diminished by the novel method. Additionally, supraclavicular region received higher maximum and mean doses in the new treatment modality compared to the conventional methods. The new method improved dose coverage for the tumor.
Raheleh Firouzi, Taher Tizdast, Javad Khalatbari, Shohreh Ghorban Shiroudi,
Volume 23, Issue 1 (3-2020)
Abstract
Background and Aim: The purpose of this study was to evaluate the relationship between stress coping strategies and difficulties in emotion regulation mediated by marital life quality in married women with breast cancer.
Methods & Materials: This is a descriptive correlational study. The study population included all married women with breast cancer in Amol and Babol cities of Iran in 2018. Of these, 385 were selected through a convenience sampling technique. The research instruments were Ways Of Coping Questionnaire, Difficulties In Emotion Regulation Scale and Marital Life Quality Scale. The goodness-of-fit of proposed model was examined by Structural Equation Modeling (SEM) in LISREL software. The indirect correlations were tested by using Preacher and Hayes’ bootstrapping method .
Ethical Considerations: This study received its ethical approval from Islamic Azad University of Tonekabon Branch (Code: IR.IAU.TON.REC.1397.029). Informed consent was obtained from all participants.
Results: The proposed model had good fit (RMSEA=0.057). All direct correlations were reported significant (P<0.05). Moreover, indirect paths between stress coping strategies and difficulties in emotion regulation through mediation by marital life quality were significant.
Conclusion: The proposed model had a good fit and can be used in identifying the factors affecting difficulties in emotion regulation. It can be useful for designing and developing programs to prevent emotions problems in women with breast cancer.
Fatemeh Tavakoli, Somayeh Reiisi,
Volume 23, Issue 2 (5-2020)
Abstract
Background and Aim: The long noncoding RNAs (lncRNAs) is an important type of RNAs that can regulate gene expression and, therefore, are involved in the development of various cancers. The genome-wide association study (GWAS) is used to identify phenotype-related loci within non-coding regions. However, the biological functions and exact relationships between phenotype-related loci and lncRNAs have not fully been identified. No study was found on the relationship between rs8506C>T polymorphisms in the lincRNA-NR_024015 exon and breast cancer susceptibility and clinical factors. Therefore, the present study aimed to evaluate the effect of polymorphism rs8506C>T on the breast cancer risk.
Methods & Materials: In this case-control study, participants were 120 patients with breast cancer, 120 healthy controls. The genetic variant was genotyped by using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR–RFLP) method. Interactions between the polymorphism and clinical factors were further evaluated, and Odds Ratio (OR) was measured for risk assessment.
Ethical Considerations: This study has been approved by the Research Ethics Committee at Shahrekord University of Medical Sciences (code:????) .
Results: There was a correlation between rs8506 C>T polymorphism and breast cancer risk in the dominant model (CC and CT+TT genotypes; P=0.027; OR=1.84; 95% CI: 1.067‐3.201). In the co-dominant model, CT genotype had a statistically significant association with breast cancer risk (P=0.038). Subjects with T allele in the rs8506 polymorphism had an increased risk of breast cancer (OR=1.69; 95% CI: 1.047-2.736; P=0.031). No relationship between rs8506 polymorphism and clinical factors including metastasis, tumor grade, and Human Epidermal Growth Factor Receptor 2 (HER2) status was observed.
Conclusion: Genetic variant rs8506 C>T polymorphism in the lincRNA-NR_024015 exon may contribute to the breast cancer risk. Allele T in this variant confers an increased risk of breast cancer. Further functional analyses are required to detect the detailed mechanism underlying the observed association.
Ali Esfahani, Shirin Zeinali, Roghayeh Kiani,
Volume 23, Issue 2 (5-2020)
Abstract
Background and Aim: Breast cancer is the most common cancer among women which leaves a profound impact on their psychosocial health and pain experience. The aim of this study was to determine the effect of group psychotherapy based on Acceptance and Commitment Therapy (ACT) on pain-related anxiety and cognitive emotion regulation of women with breast cancer.
Methods & Materials: In this quasi-experimental study, subjects were 68 women with breast cancer refereed to Shahid Ghazi Hospitalin Tabriz, Iran who were selected using a convenience sampling method. Then, they were randomly assigned into two groups of intervention (n = 34) and control (n = 34). The intervention group underwent eight 90-min sessions of ACT-based group therapy, while the control group received no any intervention. They completed Pain Anxiety Symptom Scale Short Form and Cognitive Emotion Regulation Questionnaire before and one week after treatment. Collected ata were analyzed by using t test, chi-square test, ANCOA and MANCOVA.
Ethical Considerations This study has obtained its ethical approval from the Research Ethics Committee of Tabriz University of Medical Sciences (code: IR.TBZMED.REC.1397.287) and has been registered by Iranian Registry of Clinical Trials (code: IRCT2017100615590N5).
Results: The ACT-based intervention improved the dimensions of pain-related anxiety (8.44±1.5) and cognitive regulation of negative (8.40±3.5) and positive (8.39±7.5) affects (P<0.001).
Conclusion: ACT-based intervention can help breast cancer patients to accept their negative thoughts and current conditions.
Naser Safdarian, Shadi Yousefian Dezfoulinejad,
Volume 23, Issue 2 (5-2020)
Abstract
Background and Aim: Breast cancer is the abnormal cell growth in the breast. In both benign and malignant masses, there is rapid and high cell growth. Nowadays, due to the development of technologies, the diagnosis of diseases has become non-invasive and physicians attempts to diagnose the disease without surgery and based on internal organ images.
Methods & Materials: In this study, by using images prepared from the Digital Database for Screening Mammography (DDSM), a new method is proposed for detecting cancerous masses in the mammographic images using geometric features extraction and optimization of Support Vector Machine (SVM) parameters to classify breast cancer masses automatically. First, images were pre-processed and then boundaries were determined using threshold method. Next, morphological operators were used to improve these boundaries and the segmentation of images was carried out to classify cancerous masses. Finally, by using the SVM parameter optimization method, Grasshopper Optimization Algorithm (GOA), and 4-fold crossvalidation method, data were classified into two groups of benign and malignant (cancer) masses.
Ethical Considerations Images from DDSM database were used in this research, all images are open access in this database.
Results: The accuracy, sensitivity and specificity values for applying the Radial Basis Function (RBF) kernel in SVM classifier (before optimization process) were obtained 97%, 100% and 96, respectively. After optimization of SVM parameters by the GOA, it was reported 100% for all accuracy, sensitivity and specificity indices for applying linear kernel function, indicating the high accuracy of the proposed method. The average values of accuracy, sensitivity and specificity indices for applying all three SVM kernel functions after optimization were 95.83, 100 and 94.81%, respectively.
Conclusion: The extracted geometrical features from breast cancer masses are highly efficient for model training and the diagnosis of breast cancer. The GOA could improve the overall accuracy of the proposed method by optimizing the SVM parameters. The results showed the higher performance of the proposed method compared to other methods.
Zhilla Heydarpoor, Leila Valizadeh,
Volume 23, Issue 5 (11-2020)
Abstract
Background and Aim: In the current Coronavirus Disease 2019 (COVID-19) pandemic, there have been many concerns about the transmission of the virus from the mother to the infant. This is more important due to the need for the management of breastfeeding. This study aims to review the latest studies on breastfeeding during the COVID-19 pandemic.
Methods & Materials: This is a review study on articles about breastfeeding during the COVID-19 pandemic published from December 2019 to May 2020. The search was conducted in SID, PubMed, Scopus, Science Direct, Google Scholar, Ovid, and MagIran databases using the keywords:New coronavirus, COVID-19, breast milk, and breastfeeding
Ethical Considerations: All ethical principles in writing this article have been observed according to the instructions of the National Ethics Committee and the COPE regulations.
Results: So far, there is no evidence of COVID-19 virus in breast milk or its transmission through breastfeeding. All mothers with confirmed or suspected COVID-19 should continue to breastfeed their infants in accordance with the hygiene and respiratory precautions.
Conclusion: Breastfeeding protects the baby against infectious diseases. Given that the studies reported the absence of COVID-19 virus in breast milk are very limited, this study can help mothers manage breastfeeding during the COVID-19 pandemic. Since there are still many unknowns of this emerging disease, more studies are needed on managing breastfeeding in this pandemic.
Ahmad Hamta, Sahar Adl,
Volume 24, Issue 1 (3-2021)
Abstract
Background and Aim: Breast cancer is the most common cancer type and the leading cause of cancer-induced deaths in women, worldwide. The Fibroblast Growth Factor Receptor 2 (FGFR2) is a tyrosine kinase receptor that plays an essential role in the growth, invasion, movement, and angiogenesis of tumor cells. Several single nucleotide polymorphisms have been found in the intron 2 of the FGFR2 gene, i.e., associated with a high risk of breast cancer. Genetic variation in this receptor is a new risk factor for breast cancer. The current study aimed to evaluate the association of single-nucleotide polymorphism rs2981582C/T in women with breast cancer.
Methods & Materials: In total, 80 women with breast cancer and 80 healthy women (controls) were selected from Markazi Province, Iran to participate in this research. Polymorphism rs2981582 was analyzed to investigate its association with breast cancer. DNA extraction from blood samples was performed using a kit. The presence of these single-nucleotide polymorphisms was determined by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR - RFLP). Statistical analyses were performed by SPSS using Chi-squared test at P≤0.05.
Ethical Considerations: This study was approved by the Ethics Committee of the Arak University (Code: IR.ARAKMU.REC.1395.28).
Results: Significant differences were observed in the frequency of rs2981582 polymorphism in the FGFR2 gene between the control and patient groups (P=0.000). In the patient group, the TT genotype was significantly associated with the risk of breast cancer (P=0.001; OR=3.566). On the other hand, allele C indicated a protective role against the disease (P=0.000).
Conclusion: The obtained data revealed a significant relationship between rs2981582 C/T polymorphism and the risk of breast cancer; thus, this single-nucleotide polymorphism could be used as a biomarker to predict breast cancer.
Seifollah Aghajani, Sahar Khoshsorour, Sara Taghizadeh Hir,
Volume 24, Issue 1 (3-2021)
Abstract
Background and Aim: Breast cancer is among the most prevalent cancers in women with extensive associated biopsychological problems. The present study aimed to determine the effects of holographic reprocessing therapy on cognitive flexibility and posttraumatic growth in women with breast cancer.
Methods & Materials: This was a quasi-experimental study with pretest-posttest and a control group design. The statistical population included all patients with breast cancer who underwent mastectomy and who were being treated in the chemotherapy ward of Imam Khomeini Hospital in Ardabil City, Iran, in 2020.
By convenience sampling method, 30 women with breast cancer were selected and randomly divided into the experimental and control groups (n=15/group). The experimental group, while receiving pharmacotherapy, collectively underwent holographic reprocessing therapy in 9 one-hour sessions (two sessions per week) for 5 weeks; however, the control group received only pharmacotherapy. Both research groups were assessed in pretest and posttest phases by the Cognitive Flexibility Inventory (Dennis & Vander Wal, 2010) and the Posttraumatic Growth Inventory (Tedeschi & Calhoun, 1996). Finally, the obtained data were analyzed using Multivariate Analysis of Covariance (MANCOVA) in SPSS v. 23.
Ethical Considerations: All study subjects provided a signed written informed consent form. The Research Ethics Committee of Ardabil University of Medical Sciences approved this study (Code: IR.ARUMS.REC.1399.393).
Results: The current study results indicated that holographic reprocessing therapy was effective on cognitive flexibility (F= 42.319, P<0.01) and posttraumatic growth (F= 18.300, P<0.01) in women with breast cancer; this method also increased their cognitive flexibility and posttraumatic growth.
Conclusion: Considering psychological factors and information processing methods in patients with cancer is highly important. Thus, counselors and psychotherapists are advised to use holographic reprocessing therapy as a complementary treatment without adverse effects along with medical treatments to reduce the psychosocial problems caused by the disease.
Fatemeh Sadat Hosseini Poor Abardeh, Mandana Niknam,
Volume 24, Issue 3 (8-2021)
Abstract
Background and Aim: Anxiety is one of the common disorders affecting the treatment process in patients with breast cancer. Regarding the importance of spiritual health on anxiety in cancer patients, the purpose of this study was to investigate the relationship between spiritual health with existential anxiety and morbid anxiety in the female with breast cancer.
Methods & Materials: The research method was descriptive-correlational. The statistical population included all female patients with breast cancer referred to Imam Reza Oncology and Radiotherapy Center in Mashhad in 2017. A total of 195 patients with breast cancer were selected through random sampling. Data collection tools included Beck Anxiety Inventory (1990), Lawrence & Good Existential Anxiety Inventory (1974), and Paulotzin & Ellison Spiritual Health Inventory (1982). Data were analyzed using Pearson correlation and multivariate regression.
Ethical Considerations: This study was registered by the Ethics Committee of Islamic Azad University E-Campu (Code: 950261902191).
Results: The results showed a significant positive relationship between spiritual and existential dimensions of spiritual health with existential anxiety (P>0.01). But the relationship between two dimensions of spiritual health and patients’ anxiety was not significant (P>0.01).
Conclusion: Considering the importance of spiritual health in existential anxiety and morbid anxiety, strengthening spiritual health can influence cancer patients’ anxiety.
Farzaneh Hajizadeh, Fatemeh Ebrahimi, Seyed Zia Tabatabaei,
Volume 25, Issue 3 (8-2022)
Abstract
Background and Aim The early years of life are the most critical stage in the growth and development of children. While the optimal development of children depends on a complex set of factors, the pattern of breastfeeding and complementary feeding of infants is of undeniable importance. Therefore, the present study conducted to investigate the factors related to the feeding patterns of one-year-old children referred to healthcare centers in Rafsanjan city.
Methods & Materials his descriptive study was performed on 600 mothers with one-year-old children under the auspices of healthcare centers in Rafsanjan city in 2017. Volunteered mothers with active health records were selected randomly. The required information was collected through a questionnaire containing demographic characteristics and questions related to the feeding of children and was analyzed by SPSS software, version 18.
Ethical Considerations The Ethics Committee of Rafsanjan University of Medical Sciences (RUMS) approved the present study (Code: IR.RUMS.REC.1396.179).
Results About 95% of mothers participating in this study started breastfeeding their children up to one hour after delivery, but 56.7% of them completed breastfeeding by one year of age. The results show that most mothers had started supplemental feeding with two or three spoons in each meal, and about 90% of them followed the health worker's advice. Also, the most important reasons for feeding children with powdered milk are disorders in the child's development (34%), inadequate breast milk (17%), maternal disease (14%), do not take the mother's breast (11%) and children being twins (8%).
Conclusion Despite face-to-face training for mothers in health care centers for feeding their children, still there are some difficulties in commencing complementary feeding and its continuation. Therefore, need for more health and nutrition education regarding the best ways for feeding children to mothers by personnel of Rafsanjan health care centers.
Seyedeh Zahra Shifteh, Doctor Ahmad Hamta,
Volume 26, Issue 1 (4-2023)
Abstract
Introduction: Breast cancer is a highly heterogeneous disease. The antigen molecule of four cytotoxic T-lymphocytes is involved in inhibition of T cell response and immune response regulation. Single nucleotide polymorphisms in the CTLA4 gene can affect the expression of the aforementioned molecule. The aim of this study was to investigate the polymorphisms of rs4553808 and rs733618 of CTLA4 gene with the risk of breast cancer.
Methods: In this study to investigation polymorphisms, the DNA of 80 patients with breast cancer and 80 healthy individuals in central province of ARAK were extracted from peripheral blood. Then, PCR-RFLP technique was used. The results were analyzed using SPSS software and SNP Analyzer. This study was approved by the Ethics Committee of the Arak University (Code: Ir.arakmu.rec.1396.25).
Results: Statistical analysis rs4553808 polymorphism showed no significant increase in the risk of patients with GG genotype compared with the control group (OR = 2/013, CI = 95% 1/721-2/353). Also, heterozygotes AG genotype analysis did not show any relationship between the genetic diversity and breast cancer (OR = 1/204, CI = 95% 0/604-2/402). The combination of AG + GG genotypes did not show any significant correlations (OR = 1/130, CI = 95% 0/569-2/242). Statistical analysis for rs733618 polymorphism showed increase in the risk of breast cancer. The results indicate that the TC (OR = 2/992, CI = 95% 1/280-1/998) showed a significant relationship between the genetic diversity and breast cancer. The analysis of the combined CC and TC genotypes was associated with increased risk for breast cancer compared to TT genotypes (OR = 0/334, CI = 95%; 0.143-0.782, P = 0.009). Considering that the distribution of CC and TC genotypes was significant between the two groups of control and the patient, so the frequency of TT genotype with the same amount of P = 0.001 was significant between the two groups of control and the patient.
Conclusions: There was a significant relationship between the genotypes rs733618 polymorphism and breast cancer. However, there was no significant relationship between rs4553808 polymorphism and breast cancer risk.
Zahra Ghanbari Zarandi,
Volume 27, Issue 3 (7-2024)
Abstract
Introduction: Breast cancer causes psychological problems and decreases psychological health in people, therapeutic interventions are needed to solve these problems. This research was conducted to determine the effectiveness of group meaning therapy intervention on the quality of life, emotional regulation, and life expectancy of women with breast cancer.
Methods: The research method was semi-experimental with a pre-test-post-test design with a control group. The statistical population included all women with breast cancer referring to the health centers of Kerman city in 2023. Among them, 30 people were selected by the available sampling method and were replaced randomly and by lottery in two experimental and control groups (15 individuals in the experimental group and 15 in the control group). The people in the experimental group received group-meaning therapy intervention (10 sessions) and two sessions weekly for 90 minutes. The questionnaires used in this research included quality of life, emotion regulation, and life expectancy questionnaires administered in two phases: pre-test and post-test.
Results: The results showed that before the intervention, there was no significant difference between the average scores of the quality of life, emotion regulation, and life expectancy of the intervention and control groups, but there was a statistically significant difference between the average scores of the intervention and control groups after the intervention. The mean and standard deviation of the quality of life in the post-test was 6.91 ± 29.75, emotion regulation was 10.25 ± 98.28, and life expectancy was 6.49 ± 39.51 (P < 0.001).
Conclusions: According to the results of this research, group therapy increased the quality of life, emotion regulation, and life expectancy in women with cancer. Since the favorable mental state of the patient affects their recovery, the use of this method is recommended as an effective strategy to reduce the psychological problems of patients.
Haniyeh Mozafari, Farah Lotfi Kashani, Shahram Vaziri, Mohammad Ismail Akbari,
Volume 27, Issue 3 (7-2024)
Abstract
Introduction: The present study was conducted to analyze the focal relationship of the contribution of mental adaptation to cancer, health literacy, and perceived threat in predicting the early diagnosis of breast cancer.
Methods: This study was a descriptive correlational research. The statistical population of the present study included all women with breast cancer under treatment at Shohadaye Tajrish Hospital Cancer Treatment Center in 2023, and 310 people were determined to participate in the research using G*Power software and the available sampling method. They responded to Williams, Templin, and Hines's breast cancer literacy questionnaires, Champion's perceived threat questionnaire, Watson's mental adaptation to cancer and early diagnosis questionnaire. The data were also analyzed using a focal correlation test and multiple regression.
Results: The results showed that there is a significant relationship between the contribution variables of mental adaptation, perceived threat, and health literacy with the components of early cancer diagnosis (knowledge, attitude, and actions) (P ≤ 0.01). Also, the results showed that health literacy was 4.8%, mental compatibility was 3.4 percent and perceived threat 18.7% alone predicted the variance of early diagnosis of breast cancer. The perceived threat share is 38.9%, higher than all other variables.
Conclusions: Based on the statistical results, it can be said that increasing mental adaptability, perceived threat, and health literacy play a role in early disease diagnosis, and the importance of perceived threat is more significant than mental adaptability and health literacy, which should be considered.
Rana Noruozi Kuma Olya, Sima Nasri, Samad Farashi-Banab, Fereshteh Dadfar, Naeimeh Dehghani,
Volume 27, Issue 4 (10-2024)
Abstract
Introduction: Breast cancer is one of the most common cancers and the principal cause of death in women. One of the mechanisms of cancer cells for the lack of access to the immune system is the production of compounds suppressing immune responses, such as interleukin-10. On the other hand, vascular endothelial growth factor, by binding to its receptors on the surface of endothelial cells, plays a significant role in vascular permeability and tumor vascularity. In this study, the expression of interleukin-10 and vascular endothelial growth factor in breast tumor tissue was investigated in an experimental tumor model.
Methods: First, mammary tumors were experimentally induced in Balb/C mice, and RNA was extracted from the tumor tissue. cDNA was synthesized from the extracted RNAs, and the expression level of 10-IL and VEGF genes was evaluated by RT-PCR.
Results: The results of data analysis showed that the expression of IL-10 and VEGF genes in the tumor tissue was higher than in the cells of the control group, but this increase in expression was not statistically significant.
Conclusions: In general, the expression level of Interleukin-10 and VEGF genes was increased in the experimental tumor model, but broader research and the correlation with other involved factors seem necessary.
