The hepatitis C diagnosis is based on the determination of reactant antibodies with recombinant proteins. The method which is performed for HCV screening in all blood transfusion departments. This research is conducted as a cross-sectional study from Aug 97 to Jun 99, by referring to blood donors questionnairs in central department of blood transfusion of Markazi Province. The prevalence of HCV in healthy- blood donors is 0.4% using Elisa and 0/2% using RIBA. PPV of elisa is 55%. PPV of this method is the same in both sexes. There is no relation between positive result for HCV and demographic factors. The older blood donors have v, the less chance of positive result for RIBA method. Due to the significant difference between Elisa results approved by RIBA, it is necessary to revise the ways of conducting these tests and their reliabilities.
