Background: because of the side effects of chemical drugs, using plant drugs was considered during recent decades. Based on previous studies, various concentrations of plant extracts can reduce ileum contraction caused by KCl and carbachol. Therefore, in this study, the role of muscarinic receptors in anti-spasmolytic activity of hydroalcohlic extract of parsley(petroselinum crispum)seed in adult male rat's ileum was investigated.
Materials and Methods: The distal part of Wistar rat’s ileum was separated and its contractions were recorded under 1 gr stretching and 37oC temperature in the bathroom containing the solution of Tyrode by Isotonic method. The ileum tissue was inserted in separately in first group affected by KCl and parsley seed, in the second group affected by KCl and carbachol with concentration 10-2 mg/ml and after that parsley seed, the third group affected by carbachol with concentration 10-2 mg/ml and then the extraction of parsley seed and the fourth group affected by scopolamine with concentration 10-3 M and after that KCl and then the extraction of parsley seed.
Results: Non-cumulative concentrations of extract reduced the ileum contraction caused by KCl (60mM) in a concentration-dependent form (p<0.001). There was a significant difference between the effect of extract in the presence of carbachol and extract concentration 0.2 mg/ml (p<0.01). There was no significant difference between the effect of extract in the presence of carbachol without stimulating tissue by KCl (60mM) with extract concentration 0.2mg/ml (p<0.05). There was no significant difference between the effect of extract in the presence of scopolamine with extract concentration 0.2mg/ml(p<0.05).
Conclusion: This study showed that the hydroalcholic of parsley seed extract has a relaxatory effect on rat’s ileum. Carbachol with KCl has a synergistic effect on smooth muscle contraction of the ileum wall and muscarinic receptors do not involve in the inhibitory function of parsley seed extract.
