Showing 11 results for Tahmasebi
Mohammadreza Baiatiani, M Fatemeh Seif, Mohammad Javad Tahmasebi, Mansur Ansari, Amir Sohrabi, Fakhri Sadat Hosseini,
Volume 11, Issue 4 (12-2008)
Abstract
Background: Radiotherapy is one of the cancer treatment methods. Prescribed dose for each fraction is considered based on radiosensitivity of tumoral and normal tissues. Viral agents are the effiectiv factors on tissue sensitivity. This research aimed to determine the effect of ionizing radiation of Cobal 60 on radiosensitivity of Hela cells infected with Measles virus. Methods and Materials: In this study, the radiosensitivity of Hela cells is investigated experimentally and qualitively. The cells have been cultivated in two groups (experimental and blank) and plating efficiency has been obtained. Then 100λ measles virus with serial dilution method was used to induce infection in different ratio for experimental group. After cell growth and passage, the two groups were irradiated with 2Gy gamma radiation of cobalt 60. Results: Results respectively indicated cell death increases up to 5-7%, 15-20% and 50-65%, after 2Gy irradiation by Co 60 for contaminating to Measles in low, moderate and high concentrations. Conclusion: Radiosensitivity of tumoral cells increases when they are infected by viral agent. The result in radiotherapy of cancers showed, in prescribing dose fraction non cancer disease should be considered.
Mohamad Javad Tahmasebi Birgani, Fatemeh Seif, Mohamad Reza Bayatiani, Mansour Zabihzadeh, Seyed Mohamad Hosseyni,
Volume 16, Issue 8 (11-2013)
Abstract
Background: In this research dosimetric parameters of mercury was investigated as a radiotherapy modulator and compared with cerrobend as a common beam attenuator material in most radiotherapy centers. Also several designs for mercury shielding system were evaluated.
Materials and Methods: In this experimental research transmission factor of different thicknesses of cerrobend and mercury at 6MV and 18MV photon beams was measured in order to investigate mercury as a beam modulator. Relative dosimetry was done with ionization chambers and then mercury modulating system for radiotherapy was designed with different cross sections.
Results: The result show that the transmission values increased with field size increment due to increased scatter contribution, for all cerrobend and mercury thicknesses at two energies. The procedure of mercury transmission factor is in agreement with cerrobend transmission factor. The second designed system with hexagonal cross section is in good agreement with patient contour.
Conclusion: The experimental investigation on mercury dosimetric parameters showed that mercury can be proposed as a modifier in radiotherapy because of its profit properties such as being liquid in room temperature, power of attenuation and …. So mercury can be proposed as a beam modifier (at closed system because of its poison vapor) in radiotherapy. Keywords: beam modifiers, Cerrobend, dosimetry, Mercury
Hadi Darvishi Khezri, Tahmasebi Homeyra ,
Volume 17, Issue 10 (1-2015)
Abstract
Background: Ventilator-associated pneumonia is the most frequent infection occurring in mechanically ventilated patients in the intensive care unit. Ventilator-associated pneumonia is a serious issue and remains a significant clinical problem for critically ill patients. The aim of this review article is to evaluate studies to conclude the best existing evidence for providing oral hygiene to intensive care unit patients receiving mechanical ventilation, determine the clinical evidence considering chlorhexidine, and to document a research to improve care interventions used for oral care in the critical patients.
Materials and Methods: Searching was performed in the databases: Sciencedirect, SID, CINAHL, Google Scholar, Cochrane Library, Springer and PubMed for accessibility to the studies carried out regarding with chlorhexidine mouthwash and ventilator-associated pneumonia between 1991 to 2014 years by using keywords: chlorhexidine mouthwash and ventilator-associated pneumonia.
Results: Although oral care with chlorhexidine cause a reduce the incidence of ventilator-associated pneumonia, but no evidence exists on decrease of mortality in patients undergoing mechanical ventilation admitted in the intensive care unit following its using. Conclusion: Base on this current review article can be said that protective effect of chlorhexidine mouthwash has proved in oral health and prevention of ventilator-associated pneumonia in the critical patients, but unfortunately no evidence exists to the appropriate protocol for using of chlorhexidine mouthwash in these patients.
Parisa Tahmasebi, Seyed Reza Kazemi Nezhad , Mohammad Amin Tabatabaiefar, Javad Mohammadi Asl, Nader Saki,
Volume 19, Issue 6 (9-2016)
Abstract
Background: Hearing loss is a common sensory impairment in humans which half of its causes are genetic reasons. Genetic hearing loss can be divided into the two types of syndromic and non-syndromic, which 80% of non-syndromic cases is Autosomal Recessive Non-Syndromic Hearing Loss. The aim of the present research is to determine the contribution of DFNB2 locus (MYO7A gene) in causing an autosomal recessive hearing loss in the one group of the deaf families of Khuzestan province.
Materials and Methods: This study was conducted on 26 families with autosomal recessive hearing loss (with 4 patients) and negative for GJB2 mutations in Khuzestan province. 22 families suffered from ARNSHL and 4 families suffered from Usher syndrome. Linkage analysis was performed by using STR (Short Tandem Repeat) markers related to DFNB2 locus. Each family’s genotype was determined by PCR-PAGE method. Furthermore, haplotypes drawing and LOD score calculations were performed.
Results: From 26 families with hearing loss participating in this research, following genetic linkage analysis and haplotypes drawing, two families (7.7% of the families) showed linkage to DFNB2 locus. One family (4.5%) suffered from ARNSHL and another family suffered from Usher syndrome.
Conclusion: The results of the present research show that the contribution of DFNB2 locus in causing hearing loss in the population of Khuzestan province was similar to other studies conducted in Iran and this locus with other important loci should be considered to check in the hearing loss panel.
Mohammad Bokaeian, Javad Adabi, Behroz Zeyni, Hamed Tahmasebi,
Volume 19, Issue 11 (2-2017)
Abstract
Abstract
Background: Aminoglycosides are used as antibiotics in combination with beta-lactamas for many treatments of staphylococcal infections. Development of resistance in resistant strains can be done by enzymes produced by effective genes that cause the destruction of aminoglycoside antibiotics. The aim of this study was to investigate the prevalence of the aac (6 ') Ie / aph (2 "), aph (3') - IIIa1, ant (4 ') - Ia1 genes and mecA in staphylococcus strains which play an effective part in the resistance of aminoglycosides.
Materials and Methods: in this descriptive cross-sectional observation, 113 clinical samples including 68 isolate of Staphylococcus epidermidis and 45 isolate of Staphylococcus saprophyticus of 459 clinical samples were identified by biochemical and molecular tests. The antibiotic susceptibility pattern of isolates was determined using MIC method by E-test strips. Then, to determine the presence of genes responsible for resistance to aminoglycosides, gene-specific primers were used.
Results: Of 68 isolates obtained from saprophyticus Staphylococcus aureus, 39isolates(57.35%) were mecA gene. As well, 13 isolates (19.11%) have aac (6 ') Ie / aph (2 ") gene, 9 isolates (13.23%) have aph (3') - IIIa1 gene and 7 isolates (7.3%) have ant (4 ') - Ia1 gene. Of 45 isolates of Staphylococcus saprophyticus, 23 isolates(51.11%) have mecA genes, 8 isolates (17.77%) have aac (6') Ie / aph (2 ") gene, 4 isolates (8/8%) have aph (3 ') - IIIa1 gene and 2 isolates (4.4%) have gene ant (4') - Ia1 gen.
Conclusion: Statistical analysis showed that the prevalence of aminoglycoside genes is more among strains resistant to methicillin and this would suggest that methicillin-resistant strains are easy situation for the acquisition of resistance to other antibiotics.
Mahtab Khosrofar, Mohammad Reza Pourreza, Samira Asgharzadeh, Parisa Tahmasebi, Elahe Ali Asgari, Reza Ghasemikhah, Nader Saki, Javad Mohammadi-Asl, Morteza Hashemzadeh Chaleshtori, Mohammad Amin Tabatabaiefar,
Volume 20, Issue 3 (6-2017)
Abstract
Abstract
Background: Hearing loss (HL) is the most common congenital defect in humans. One or two in thousand newborn babies have prelingual hearing loss. Autosomal recessive non-syndromic hearing loss (ARNSHL) is the most common form of hereditary deafness. Hearing loss is more common in the developing countries which is due to genetic and environmental (cultural -health factors) reasons. HL has a wide range of clinical demonstrations including: congenital or late onset, conductive or sensory-neural, syndromic or non-syndromic hearing loss. The goal of this project is to determine the portion of the DFNB21 (TECTA) in ARNSHL in families with negative GJB2 gene in Khuzestan province.
Materials and Methods: We studied 21 families with ARNSHL with at least 4 patients and negative for GJB2 mutations from Khuzestan province. Genetic linkage analysis was performed using STR markers linked to DFNB21 locus.
Results: Following genetic linkage analysis and haplotyping, out of 21 families with ARNSHL, one family showed linkage to the DFNB21 (TECTA) locus.
Conclusion: The results of this project confirm other studies in Iran and give insight into the most common loci causing ARNSHL in Iran which could be helpful in research and clinic.
Hamid Motamedi, Shahnaz Dehbashi, Hamed Tahmasebi, Mohammad Reza Arabestani,
Volume 21, Issue 5 (10-2018)
Abstract
Background and Aim: Staphylococcus aureus(S.aureus) has many pathogens. Antibiotic resistance may increase the invasion of this bacterium. The aim of this study was to determine the role and effect of some antibiotic resistance in the spread of pathogenic strains of S.aureus in different clinical specimens.
Materials and Methods: 95 clinical isolates of S.aureus were collected from different clinical specimens. Antibiotic resistance pattern was determined by Disc diffusion method (Kirby-Bauer) for 6 different classes. Identification of adhesion agent genes in isolated isolates was performed using Multiplex-PCR and specific primers. For analysis of the results, GraphPad Prism version 6 and ꭕ2 statistical sampling was used. p≤0.05 was considered significant.
Findings: Of 95 isolates of S.aureus, 29 isolates (30.52%) were resistant to methicillin, 12 isolates (12.63%), resistant to clindamycin, 48 isolates (50.52%), resistant to gatyfloxacin, 88 (92.63%) isolates resistant to gentamicin, 57 (60%) isolates resistant to erythromycin and 79 isolates (83.15%) were resistant to tetracycline. fnbA genes were isolated in 14 isolates (14.73%), fnbB in 29 isolates (30.52%), fib in 21 isolates (22.10%), clfA in 17 isolates (17.89%) and clfB in 19 isolates (20%). There was a significant correlation between resistance to macular antibiotics, tetracycline, beta-lactam, lacosamide, aminoglycoside and pathogens.
Conclusion: The adhesion factors in S.aureus possibly cause some structural changes and cause resistance to various antibiotic classes.
Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Reza Arabestani,
Volume 21, Issue 7 (2-2019)
Abstract
Background and Aim: Gene mutation in Staphylococcus aureus is one of the most important causes of antibiotic-resistant strains. The High Resolution Melting Curve (HRM) analysis of DNA method can detect these mutations very high quality. The purpose of this study was to evaluate the role of clinical sample type in the occurrence of nucleotide mutations in the mecA gene of S. aureus by HRM method.
Materials and Methods: In this experimental study, 43 clinical isolates of S. aureus were used. To detect possible mutations, isolates with mecA gene were replicated and sequenced. Then, analysis was performed using StepOne Software v2.3 and HRM v3.0.1 software. Sequencing results were used as gold-standard.
Ethical Considerations: This study with research ethics code IR.UMSHA.REC.1396.637 has been approved by research ethics committee at Hamadan University of Medical Sciences.
Findings: Of 43 clinical isolates of S. aureus, 11 isolates (25.58%) had mecA gene and 32 isolates (47.41%) lacked the mecA gene. According to different clinical samples, 3 isolates (27.27%) were resistant to methicillin from blood samples, 2 isolates (18.18%) from urine specimens, 2 isolates (18.18%) from wound samples, 2 isolates (18.18%) of the catheter samples, 1 isolate (9.09%) of the abscess and 1 isolate (9.09%) were separated from the nose swab. In the meanwhile, isolates from the wound and urine had the highest mutation in the adenine amino acid as A → T, A → G, A → C, and
A → X. Isolates taken from blood have mutations in Guanine amino acid as
G → A.
Conclusion: There was a significant relationship between type of mutation and type of clinical specimen in methicillin-resistant Staphylococcus aureus isolates.
Saeed Tahmasebi, Sharbanoo Oryan, Hamid Reza Mohajerani, Neda Akbari, Mohammad Reza Palizvan,
Volume 23, Issue 3 (August & September 2020)
Abstract
Background and Aim: Epilepsy is a common brain disorder. Brain function can be affected by the intestinal microflora. The intestinal microflora have a major role in modulating immune responses, producing essential metabolites and mediating neurotransmitters. Antibiotics can affect and reduce these roles and functions by eliminating the intestinal microflora. The aim of this study was to investigate the effect of intestinal natural micro flora removal on seizure susceptibility and seizure behavior modification with the use of probiotics in male Wistar rats.
Methods & Materials: This study was performed on 32 male Wistar rats with weight range 200-250 gr. The animals were randomly divided into four groups: 1. Control group; 2. Antibiotic group; 3. Probiotic group and 4. Antibiotic + probiotic group. To remove the microflora, antibiotics (neomycin, ampicillin, and metronidazole) for three weeks and for replacement of microflora, probiotics (Lactobacilli casei, Lactobacillus acidophilus and Bifidobacterium bifidum) for four weeks were administered. Seizures were performed by intraperitoneal injection of pentylentrazole. The microflora was examined by the MRS Agar medium and the Pure Plate method. The data were statistically analyzed in Graph Pad Prism V. 8.
Ethical Considerations: This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1395.176).
Results: The use of antibiotics lead to decrease the number of intestinal bacteria (P<0.0001), increased the severity and stability of seizure stages (P<0.05) and decreased the time delay of seizure onset (P<0.05) compared to the control group. Probiotic consumption by modifying the intestinal microflora (P<0.0001) reduced the severity of seizure and increased the time delay of seizure onset (P<0.05).
Conclusion: Elimination of microflora has the potential to induce seizures, which can be compensated by administration of probiotics.
Dr Banafsheh Ghaheri, Dr Shahzad Tahmasebi Boroujeni, Dr Mehdi Shahbazi, Dr Ahmad Reza Arshi,
Volume 24, Issue 6 (February & March 2022)
Abstract
Background and Aim: Evaluating variability can help to investigate the process underlying motor coordination problems. The current study aimed to measure motor coordination and its variability in children with Developmental Coordination Disorder (DCD). Moreover, the symmetry of motor coordination variability in these children and the relation between motor skills and variability were explored.
Materials and Methods: After evaluating motor skills in children, aged 7-10 years using Movement Assessment Battery for Children-2 (MABC-2), 15 children with DCD and 20 non-DCD children performed a bilateral coordination task. Using motion capture system, motor coordination and variability were recorded and calculated by computing continuous relative phase and its standard deviation, respectively.
Ethical Considerations: The study with an ethical code of IR.UT.SPORT.REC.1396030 was approved by Ethics Committee of Faculty of Physical Education and Sport Sciences of University of Tehran.
Findings: Children with DCD showed significantly higher variability, while there was no significant difference between the groups in performing the coordination task. Moreover, the variability of motor coordination was found asymmetrical in children with DCD. Finally, more variability was shown to be accompanied with lower score in motor skills of the participants.
Conclusion: The current study shows the necessity of employing assessments related to underlying process of movement coordination such as variability, which can help to provide more comprehensive understanding of motor patterns of children with DCD and the strategies that they adopt to execute and produce movement.
Heshmatallah Alikarami, Saeedeh Tahmasebi, Mohammad Fathi, Raziyeh Rezaei,
Volume 26, Issue 6 (February & March 2024)
Abstract
Abstract
Introduction: Resistance training (RT) is an important part of athletes' preparation exercises with the aim of strengthening physical fitness, creating neuro-muscular adaptations and improving sports performance. The aim of this study was to investigate the effect of resistance training with weight on NCV of tibial and peroneal nerves, muscle strength, agility, flexibility and performance of half-back, half-turn and angel balance skills in seven- to ten-year-old gymnasts.
Methods: In this semi-experimental study, 24 children with two years of experience in gymnastics (age: 8.5±1.5 years, weight: 32.10±9.40 kg, height: 135±13 cm) voluntarily Participated. Subjects were randomly divided into two groups including gymnastics training and gymnastics training + resistance training with weights. And they trained for 10 weeks. During the research, one group did general gymnastics exercises, and the other group did resistance training with weights three times a week in addition to gymnastics exercises. Before and after the exercises, NCV test, Sargent's jump muscle power tests, agility of 9x4m back-and-forth run, Wells' flexibility, and performing half-back, half-turn and balance skills of Angel were performed. Independent t-test was used to analyze the data. And a significance level of P<0.05 was considered.
Results: The results showed that the NCV in the tibial and peroneal nerves increased significantly in the weight resistance training group compared to the general gymnastics training group. Also, muscle strength in Sargent's jump, agility in 4x9m back and forth running, and the performance of half-back and half-turn skills increased significantly (P<0.05). However, no significant change was observed in the flexibility with the Wells test (P=0.870) and execution of Angel's balance skill (P=0.552).
conclusion: 10 weeks of resistance training with weights significantly increases neuromuscular adaptations, NCV, muscle power, agility, and implementation of half-back and half-varus skills in gymnast children. Therefore, it is suggested to use resistance exercises with weights to improve the performance of children athletes in the field of gymnastics and fields where nerve conduction speed, muscle power and agility are effective in the success of athletes.
