Showing 9 results for Soufian
Masoume Soufian, Farshideh Didgar,
Volume 2, Issue 8 (Autumn 1999)
Abstract
Cholera is potentially dangerous bacterial infection of the small intestine which may be serious enough to cause death, cholera epidemics cause economical and hygienic problems. In retrospective study, 54 cases were consider during 4 months admitted in Valie -asr hospital. Results were as follow:
There was no significant changes due to sex. Sixty percent of cases were above 50 years. 78% showed vomiting, 11% fever and bloody diarrhea which in half of above cases ameobic dyscentery was observed. In stool examination we observed 32% WBC, 14% RBC and 19% WBC along with RBC. Cell was not seen in 35% of the cases in 5.5% ameobic trophozoite observed. Hypokalemia were observed in 80% acidosis 21% and ATN in 50% which 82% of that resolved after treatment. In all cases with leukocytosis above 20000, ATN have been observed. Rate of ATN and mortality in patients above 50 years significantly increased (4.9) and mortality and time of admission and observing initial symptoms. Due to above results may suggested that, leukolytosis above 20000 may be consider as a prognostic factor in incidence of ATN in above cases, also existence of WBC or RBC in stool smear can not denial eltor.
Masoume Soufian,
Volume 3, Issue 2 (Summer 2000)
Abstract
Brucellosis is a common disease in Markazi province, however meningitis of Brucella is rare. The patient was a -25- year old woman who was referred to Vali-Asr Hospital for the loss of consciousness. She had heache, vomiting and fever since two months ago. The lab tests showed positive wright in serum and CSF. The patient was recovered by the treatment of Brucellosis. The reason of rarity of Brucella meningitis, is probably that the disease which is endemic in Markazi province and an early diagnosis and treatment will prevent it from complications, especially menegitis. This case is reported because of its rarity.
Masoume Soufian,
Volume 3, Issue 4 (Winter 2000)
Abstract
Familian meditranian fever (FMF) is an inherited disorder and characterized by recurrent self limiting attacks of fever and polyserositis. Because of rarity of this disease and the difficulty of diagnosis, the physicians presence of mind is a necessary thing. The patient is a 16 years old boy who had recurrent attacks of abdominal pain, headache, fever, rash and arthritis. The interval of his attacks was variable between 1-2 years and 2 times in a week. He had an appendectomy about 8 years ago. Because of arthritis, with probable diagnosis of R.F, he received penicillin monthly. During the attacks, lab tests showed, leukocytosis elevated sedimentation rate and positive CPR.
The CT of abdomen and transient of small bowell were normal.
The use of colchicine 0.5mg two times in a day caused arrest of attacks.
This case is reported because of its rarity and for physicians presence of mind.
Farshideh Didgar, Majid Akbari, Hossein Sarmadian, Masoomeh Soufian,
Volume 13, Issue 3 (9-2010)
Abstract
Background: Brucellosis, a zoonotic disease with worldwide spread, is a common disease in Iran and is endemic in Markazi Province. Brucella osteomyelitis, on the other hand, is a rare disease. Here we reported a case of brucella osteomyelitis of the tibia following trauma. Case: The patient was a 22 year old man referring with chronic draining tibia ulcer. He had a history of trauma of the tibia, dating back to 3 years ago, for which local prosthesis had been prescribed. Later on, the prosthesis had been removed due to infection. Staining of the tissue samples indicated the presence of gram-negative coccobacill and brucella growth in the culture of excretions. Conclusion: This case was reported because of rare presentation of brucella and the educational implications of the patient with respect to the possibility of brucella development in chronic ulcers or prosthesis. Although uncommon, brucellosis should be regarded as a potential cause of osteomyelitis in patients with a record of physical contact with animals.
Safyeh Soufian, Masomeh Sofian,
Volume 13, Issue 5 (Supplement of Quran and Medicine 2011)
Abstract
Background: Recent advances in biology and medicine have revolutionized human knowledge on the brain and neurology. This has resulted in the emergence of psychological theories on religious beliefs and experiences in different cultures. This review article deals with religious experiences from a neurologic perspective. Materials and Methods: Functional imaging techniques such as SPECT, positron emission tomography (PET), and functional MRI (fMRI) allow for the study of brain functions of religious individuals. Religious acts activate a circuit in the brain site which is known as religious circuit that involves the amygdale, the hippocampus, the limbic system, the anterior temporal lobe, the orbito-frontal, and dorsomedial and dorsolateral prefrontal cortices. The religion circuit is regulated by serotonin and dopamin. Long-term religious exercises, such as meditation, activate the frontal lobes which give human beings greater control over the functions of the “self”. The word “self” has been referred to as the nafs in Quran which consists of ‘that which incites to evil’ (alnafs al-ammara), ‘the nafs that blames’ (al-nafs al-lawwama), and ‘the serene self’ (al-nafs al-mutma’inna). Conclusion: Survival of ethical behaviors belonging to the inhibitory behavior depends on the formation of brain connections which can only be obtained through consistent long-term religious exercises.
Leila Hasanzadeh, Hamid Abtahi, Ehsanollah Ghaznavi-Rad , Safieh Soufian , Vahideh Farjadi ,
Volume 16, Issue 1 (4-2013)
Abstract
Background: Helicobacter pylori is the most common bacteria causing chronic infections worldwide. An important virulence factor of H. pylori is a vacuolating cytotoxin (VacA) that induces the formation of acidic vacuoles in cytoplasm and damage to epithelial cells. The aim of this study was to examine the antigenic properties of the recombinant VacA of H. pylori in infected sera of mice and human.
Materials and Methods: In this experimental study, the highly antigenic region of VacA gene (1233 bp) was detected by bioinformatics methods, and it was amplified by PCR method and cloned into the pET32a expression vector. After expression and purification of the target protein, its antigenicity was studied by Western Blotting using human sera infected with H. pylori and sera from immunized mice infected with purified recombinant VacA.
Results: PCR and sequencing results showed that the target gene was correctly cloned into the recombinant vector. Antibodies used in Western Blotting indicated the production and expression of the recombinant protein (65kDa) with concentration of 2.1 mg/ml.
Conclusion: Recombinant VacA protein has antigenic and immunogenic properties thus, it is a proper candidate for designing H. pylori vaccine and diagnostic kits
Vahideh Farjadi , Hamid Abtahi, Mohammad Reza Zolfaghari, Safieh Soufian, Leila Hasanzadeh,
Volume 16, Issue 7 (10-2013)
Abstract
Background: Helicobacter pylori (H. pylori) is a gram negative bacilli that causes the stomach and duodenum diseases in human. An important virulence factor of H. pylori is a CagA gene that increases of colonization it in stomach epithelial cells and lead to inflammation and peptic ulcers. The aim of the present study was to production of recombinant protein containing highly antigenic region of CagA in E. coli.
Materials and Methods: In this experimental study, the antigenic region (1245 base pair) of CagA gene was detected by bioinformatics methods, proliferated by PCR method, digested by BamHI and XhoI restriction enzymes and cloned into pET32a plasmid and was expressed in the E. coli BL21 (DE3) pLysS with induced by IPTG. The expressed protein was purified with Ni-NTA kit and its antigenicity was studied by western blotting method.
Results: Data showed the successful cloning and expression of the target gene. Recombinant CagA protein purified by Ni-NTA kit and dialysis with concentration of 1.5 mg/ml. In western blotting, the produced protein was interacted with infected human and mice sera.
Conclusion: Results indicated that recombinant CagA protein (65 KDa) maintains its antigenicity, so could be used for serological diagnosis of H. pylori diseases and production of vaccine.
Nafise O Sadat Mirjamali Mehrabadi, Safieh Soufian, Hamid Abtahi,
Volume 17, Issue 4 (7-2014)
Abstract
Background: Streptococcus pyogenes produce extracellular hyaluronidase enzyme which is directly associated with the spreading of the organism during infection. Hyaluronidase enzyme is able to break hyaluronic acid or interstitial cement. This enzyme might be used in cancer treatment.The objective of the present study was to clone and express the nucleotide sequence of this enzyme which is involved in hyaluronidase enzymatic activity.
Materials and Methods: The enzymatic region of hyaluronidase gene was detected by bioinformatics methods. The polymerase chain reaction method was used to amplify the region. The amplified product was cloned into the expression vector pET32a. E. coli BL21 (DE3) pLYsS was transformed with recombinant plasmids. Then gene expression was induced by IPTG. The expressed protein was purified successfully via affinity chromatography by NiNTA kit. The integrity of the product was confirmed by western-blot analysis.
Results: The nucleotide sequence of amplified gene was consistent with the streptocuccal hyaluronidase gene. The concentration of recombinant protein calculated to 500 mg purified protein per liter. The enzymatic region of recombinant protein from Streptococcus pyogenes was recognized by all five patient’s sera with Streptococcus infection.
Conclusion: In general, it is possible to produce the enzymatic regions of the Streptococcus pyogenes hyaluronidase in Escherichia coli. The antigenic property of the produced protein is well retained. Considering the product's domestic demand and also low efficiency of production and pathogenicity of Streptococcus species, it is possible to produce it as recombinant product.
Nader Zarinfar, Maryam Valikhani, Bahman Sadeghi, Masoumeh Soufian, Majid Akbari,
Volume 19, Issue 11 (2-2017)
Abstract
Abstract
Background: Probiotics are known as a factor for prevention of infectious diseases such as urinary tract infection(UTI). The present study aimed to investigate the positive effects of probiotics on urinery truct infection of cateterized intensive care unit patients.
Materials and Methods: In a triple blind clinical trial, 125 cateterized intensive care unit patients were randomly selected and divided into two groups, Treatment group (n = 63) was well matched with placebo group (n = 62) for age and sex and duration of admission .Case group received one lactocare capsule for 10 days and control group with a same method received placebo for 10 days. In both groups urine analysis and urine culture were measured with a same method at the first day and then twentieth day and finally data were analysed with t-test.
Results: The incidence of urinary tract infection between treatment and control group (respectively 17.4% and 22.5%) had no statistically significant difference (p=0.672). But, in terms of sex, incidence of urinary tract infections in women compared to men with the same condition was significantly different(p=0.002). In treatment group with long term cathetrization, urinary tract infections significantly were significantly different(p=0.041).
Conclusion: Administration of lactocare capsule has no statistically significant impact on the incidence of urinery truct infection of cateterized intensive care unit patients. In comparsion of cateterized men and women receiving probiotics, the reduction of urinary tract infection in women was more than in men. Probiotics had preventive effects on long term catheterization.
