Showing 13 results for Shokri
Ghasem Mosabebi, Fazel Shokri,
Volume 1, Issue 2 (Spring 1997)
Abstract
The total of IgM and IgA in serum and synivial fluids of 45 patients with Rheumatiod Arthritis (RA) are measured by critical method of ELISA. %93 of the patients had RF in their serum. The results show that there is no difference between the rate of IgMRF and IgARF in serum and synovial fluid.It was found that percentage Rf the ratio in serum is higher than that of synovial fluid by the calculation of Re titre to total immunogloboline. The fifference is significant from statistical point of view. The main places of RF production probably is in the joins of patient who are contracted.
Ghasem Mosayebi , Kamran Moshfeghi, Mohammad Moazzeni, Fazel Shokri ,
Volume 7, Issue 4 (Winter 2004)
Abstract
Introduction: different isotypes of antibody can be produced by immune system after antigen contact. Detection and measurement of different classes of antibody against the antigen is very important in some cases. The aim of this study is designing of an ELISA method on the basis of inhibition of enzyme activity by using a non-competitive inhibitor. Therefore in this study rheumatoid factor is used as a model for the detection of different other classes of antibodies against the antigen.
Materials and Methods: In this cross sectional analytical study, we measured IgM and IgA rheumatoid factors in sera of 10 patients with rheumatoid arthritis and positive latex test , by mixed and routine ELISA. In mixed ELISA the activity of the first conjugated enzyme was blocked by a non-competitive inhibitor after adding the substrate. Then the next conjugated antibody, which was specific for another isotype, was added. By optical density, results was comparisoned with routine ELISA.
Results: The obtained results showed that the average optical density is lower when compared with routine ELISA , but the difference is not statistically significant. however these two methods did not show any significant difference in quantifying antibody isotypes. Also there is a positive association between mixed and routine ELISA (r=0.9, p=0.001).
Discussion: Lower optical density in mixed ELISA is probably because of stick hindrance by the first conjugate. So, because there is no significant difference between the results of these two types of ELISA, and also no need to repeat the test for each isotype in this method, it is recommended to use the new method instead of the routine one to save time and reagents.
Behnam Ghalenoei, Ali Fani, Shima Monzavi, Masoumeh Shokri,
Volume 10, Issue 4 (12-2007)
Abstract
Introduction: Peptic ulcer is a condition in which there is an ulcer in the stomach or duodenum. The incidence of peptic ulcer is 5 to 10% and is more prevalent in men. Infection with helicobacter pylori, NSAIDS, smoking and alcoholism are of the most important risk factors. Another risk factor is the O blood group. Since most research in this field are done abroad this study is done with the aim of determining the relation between ABO blood group and Rh with peptic ulcer. Materials and Methods: In this case-control study, 325 persons with dyspepsia were investigated. These individuals were referred for endoscopy. The case group consisted of 110 persons in whom endoscopy had revealed a peptic ulcer and control group 215 persons in whom endoscopy had showed no sign of ulcer. Blood grouping was performed by slide and tube methods .Chi square test was used for data analysis. Results: Based on the results, patients’ age was between 18-75 years. 68.6% of samples were women because they had more referrals. There was a significant relation between age and sex with peptic ulcer. 25.5% of patients were single and 72.5% married. Among all samples 32.9% had A blood group, 18.5% B, 11.1% AB and 37.5% O and 88% were Rh positive and 12% Rh negative. There was no significant relation between ABO blood group and Rh with peptic ulcer. The gathered data was analyzed using odds ratio, relative risk and Chi square test. Conclusion: Although some investigations have showed correlations between ABO blood group and peptic ulcer, our data showed no correlation. This may be due to the prevalence of helicobacter pylori infection among patients with peptic ulcer which needs more investigations. Other effective factors may be life style and nutrition.
Hosein Shokri, Davood Hekmatpou, Hamid Reza Ebrahimi Fakhar, Akram Nyazi, Mohsen Azadi, Mohsen Taghizadeh,
Volume 16, Issue 7 (10-2013)
Abstract
Background: Patients with myocardial infarction are faced to multi stressors in which cause to increase their anxiety. This study with the goal of determination of the effect of Durosia Anethifolia (Barilax) on anxiety after myocardial infarction was accomplished.
Materials and Methods:This is a clinical trial which was done on 90 patients with MI. All people were selected by simple method at first and then they randomly allocated into two intervention and control groups. After 24 hours, perl of Barilax 1oo mg twice per day were prescribed for intervention group, and the same dosage of placebo were given to control group. Anxity rate 24 and 96 hours after MI by state Trait Anxiety Inventory (STAI) were measured. All data with X2 and paired T test were analysed.
Results: The mean age of patients in intervention group was 60.68 and 61.46 in control group. In intervention group 51% male and 49% were female and in control group 71% were male and 29% were female. Before intervention, the mean of anxiety rate in intervention group was 46.91 and in control group was 42.24 with no significant difference. After intervention, the mean of anxiety rate in intervention group was 33.35 and in control group was 36.48 with significant difference (p=0.012).
Conclusion: Oral consumption of Durosia Anethifolia (Barilax) on anxiety rate after myocardial infarction was effective. So description of this drug as an effective drug on controling of anxiety after myocardial infarction is recommended.
Mojgan Mirghafourvand, Aziz Homayouni Rad, Sakineh Mohammad Alizadeh Charandabi, Kolsoum Shokri,
Volume 18, Issue 8 (11-2015)
Abstract
Background: Constipation is a functional gastrointestinal disorder and the use of probiotics is considered as a new solution in prevention and treatment of constipation. The aim of this study is to review the clinical evidence about probiotics effects in the prevention and treatment of constipation.
Materials and Methods: To review the accessible evidence about efficacy of probiotics in the prevention and treatment of constipation, the search of key words such as constipation, probiotic and yoghurt are conducted in databases Pubmed, Medlinplus and Cochran from 1980-2013 and of 49 articles about constipation, 16 yandomized controlled clinical trial articles and 3 pre-test and post-test randomized trials that investigated the effects of probiotics on constipation were included in this study.
Results: Probiotics produced lactic acid and fatty acids with short chains that decrease the colon pH and stimulate muscular contractions and intestinal peristalsis. Some of probiotics can increase the mucous discharge and change linked bile salts to free bile salts entering much water to colon and leads to soft defecation.
Conclusion: Considering studies, daily use of probiotics such as lactobacillus acidophilus and bifidobacteria lactic 105-1011 CFU/gr for at least 1-2 weeks can help to decrease constipation.
Mahdi Jafarzadeh, Mojtaba Salouti, Rasoul Shokri,
Volume 20, Issue 6 (9-2017)
Abstract
Abstract
Background: Candida glabrata is the fourth most common cause of blood infection in America and, due to its resistance to amphotericin B, we have to look for new therapies. The aim of this study was to evaluate the antifungal properties of silver nanoparticles, copper and combine them together with amphotericin B in order to produce an effective drug.
Materials and Methods: Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) for nano silver copper and combining them together and amphotericin B was conducted by macro dilution. By intraperitoneal injection, anti-fungal effect of nano silver, copper and combining them together and amphotericin B was evaluated in a mouse model.
Results: The amounts of MIC and MFC for silver nanoparticles, copper nanoparticles and their combination were 31 and 62.50 ppm, 31 and 62.50 ppm ,and 31and 15.50 ppm, respectively. The amounts of MIC and MFC for the combination of silver nanoparticles and amphotericin B were 8 and 15.5 ppm, also, 15.5 and 31 ppm for the combination of copper nanoparticles and amphotericin B. Mouse model study confirmed the effect of silver nanoparticles, copper nanoparticles and combining them together and amphotericin B against Candida glabrata. The combination of silver and copper nanoparticles had maximum effect and the combination of silver and copper nanoparticles with amphotericin B had minimum effect in animal model.
Conclusion: The combination of silver and copper nanoparticles has a better effect than other groups.
Saeed Mohamadi Molod, Ali Heidarian Pour, Elnaz Shokri,
Volume 21, Issue 2 (5-2018)
Abstract
Abstract
Background: The aim of this research was to investigate the effect of substructural exercises on serum brain-derived neurotrophic factor (BDNF) and attention function in boys with attention deficit hyperactivity disorder (ADHD).
Materials and Methods: The research method was semi-experimental with pre-test and post-test design with control group. Statistical population of this research included all elementary male students who in the early reviews, the symptoms of attention deficit hyperactivity disorder were diagnosed and referred for further study and treatment to the core of counseling and psychology of education in Sardar district in academic year 2016-2017. From the above community, 30 boys aged 7 to 12 years old were selected as the sample and randomly assigned to control and experimental groups. In order to recognize the attention deficit, the Conners' Parent's Questionnaire and clinical interview were used and to measure attention, Continuous Performance Test (CPT) and to measure the serum levels of BDNF, ESTOBIOPHARM kit were used. The experimental group subjects performed aerobic exercises with intensity 45-75% of maximal heart rate for 12 weeks in 36 sessions of training, 25-75 minutes, and data were analyzed using multivariate covariance.
Results: The results showed that there was a significant difference between control and experimental groups in serum levels of BDNF and continuous attention function (p<0.001).
Conclusion: Regarding the results of this study, it can be concluded that endurance training increases the BDNF and thus improves attention in boy with attention deficit hyperactivity disorder (ADHD).
Mrs. Mahnaz Ghahramani Til, Mrs. Rezvaneh Sadat Fatemi, Dr. Rahman Shokri, Dr. Mahdi Banitalebi Dehkordi, Dr. Mahdi Paryan,
Volume 25, Issue 4 (October & November 2022)
Abstract
Introduction: Salmonella infection (salmonellosis) is a common bacterial disease that affects the intestinal tract. Several methods like Multiplex or real-time PCR, ELISA, and Agglutination are used to identify these bacteria. However, normally rapid, cost effective and easy diagnostic methods such as agglutination test is recommended. In Iran, positive control antiserums used in diagnostic kits work based on polyvalent agglutination and are against O and H antigens. The purpose of this research was to produce specific anti-sera against O and H antigens for using in agglutination and ELISA kits.
Methods: New Zealand white rabbits were immunized by intravenous injections of inactivated bacterial O and H antigens adjusted to a cell density equivalent to a turbidity of a McFarland number 3 standard. Serum collection was performed 7 days after the last injection. Collected Antisera were tested with positive human specimens as well as cross-reaction antibodies. Absorption method was used to obtain specific anti-sera against O and H antigens. Produced Anti-O and Anti-H antibodies were mixed with bacterial H and O antigens respectively and incubated for 1 hours in 37˚c. The Mixture was centrifuged and the supernatant was collected. Furthermore, in order to use these antisera in specific kits such as ELISA, Immunofluorescence etc., purification methods like Ammonium sulphate precipitation, tangential Flow Filtration and Chromatography were performed. This study was approved by the ethics committee of Pasteur Institute of Iran (Code: IR.PII.REC.1399.006).
Results: Results of agglutination test before and after adsorption showed cross-reaction before adsorption and no cross-reaction with H and O antigens with monospecific antisera against O and H after adsorption, respectively. Moreover, high quality and quantity of mono-specific antibody was obtained after purification.
Conclusions: Serum-based assays are recommended for the timely diagnosis of the disease since these assays are specific, sensitive, inexpensive, and rapid. Therefore, the produced antiserum in the present research can be used in primary screening of salmonella infections based on agglutination tests which are cost effective and simple. In addition, purified anti-sera can be used in the development of ELISA and Immunofluorescence assays.
Farnaz Ghorbani, Mostafa Ghandi, Navid Yousefi, Maryam Shokri Mojdeh,
Volume 25, Issue 6 (February & March 2023)
Abstract
Introduction: Dentists are at high risk for COVID19 disease and spreading it to colleagues, families and patients. The aim of this study was to evaluate COVID 19 epidemic anxiety among students and dental assistants of Qazvin University of Medical Sciences.
Methods: In this descriptive-Analytic cross-sectional study, 142 dental students who were willing to participate the study were surveyed. Data collection tools was included a demographic and Corona Disease Anxiety Scale (CDAS) questionnaire. After collecting information, each participant's anxiety score was measured from zero to 54. These raw scores were converted to standard T scores for comparability with other studies, and according to the scope of standard T studies, participants in one of three groups of no anxiety or mild (T≤ 39), moderate (60 ≤ T ≤ 40), And severe anxiety (T≥61), and then the data were analyzed with SPSS23 statistical software. Ethical code of this study is IR.QUMS.REC.1399.224 in Qazvin University of Medical Sciences.
Results: According to the results, the level of anxiety was higher in older age groups, among men against women, and among married against single people, respectively. Also, the level of anxiety caused by Coronaviruses pandemic was higher in undergraduate against postgraduate students. However, difference was not statistically significant (P <0.05).
Conclusions: Based on the study, it can be stated that the level of coronaviruses anxiety in dental students is mild. In these situation, measures to maintain the psychological health of students should be a necessity of the planning of the directors and professors of the dental school.
Shiva Kavousinejad, Mohadese Shokripour, Bahareh Asgartooran, Zahra Cheraghi,
Volume 26, Issue 4 (October & November 2023)
Abstract
Abstract
Background and Aim: This study aimed to compare the microleakage of an adhesive fissure sealant (AFS), a flowable composite resin (FC), and a resin-modified glass ionomer (RMGI) in permanent teeth.
Materials and Methods: This in vitro, experimental study was conducted on 45 extracted posterior teeth with sound fissures. The teeth were randomly divided into 3 groups (n=15). The occlusal fissures were sealed with Beautisealant AFS without etching and bonding in group 1, with Beautifil FC after 37% phosphoric acid etching in group 2, and with Fuji II LC RMGI in group 3. The teeth underwent 500 thermal cycles (5-55°C), and their apices were sealed with wax. They were then immersed in 2% methylene blue for 24 hours. Next, the teeth were sectioned buccolingually and inspected under a stereomicroscope to measure the dye penetration depth as an indicator of microleakage. Data were analyzed by ANOVA, Chi-square test, and Scheffe test (alpha=0.05).
Ethical consideration: The study was approved by the ethics committee of Hamadan University of Medical Sciences (IR.UMSHA.REC.1399.728).
Findings: The microleakage score of RMGI was significantly higher than that of AFS and FC (P<0.05), while the microleakage scores of FC and AFS were not significantly different (P=0.330).
Conclusion: The AFS showed a sealing ability comparable to that of FC, and can be used as an alternative to FC due to its fewer application steps.
Dr Zahra Miraki, Dr Masomeh Rostamzadeh, Mr Aras Feizi, Dr Azad Shokri, Dr Mohammad Aziz Rasouli,
Volume 26, Issue 5 (December & January 2023)
Abstract
Introduction: The dentist's level of knowledge and abilities is one of the determining factors in the correct selection and application of dental cements. This study aimed to investigate the level of knowledge, attitude, and practice of general dentists and specialists about the application of different dental cements.
Methods: This descriptive-analytical study was cross-sectional one which conducted on 150 specialist and general dentists in governmental and private centers in Kurdistan province (2019) who performed fixed prosthetic treatments, was done through census and available sampling. The data collection tool was a questionnaire. To analyze data, T-test and ANOVA tests were used in SPSS-22 software. A significance level was considered less than 0.05. Ethical considerations: This study was conducted after the approval of the protocol in the research ethics committee and receiving the ethical code number IR.MUK.REC.1398.302 and according to the Declaration of Helsinki.
Results: The overall average score of dentists' knowledge was 6.5, which was close to and higher than the average scores and had a significant relationship with the grade and place of employment (P<0.05). The overall average score of attitude was 3.4, which was almost equal to the average scores. There was a significant relationship between grade and gender with attitude score (P<0.05). The overall average score of the dentists' practice was 5, which was higher than the average scores, and the practice score had a significant relationship with gender, place of employment, and grade (P<0.05).
Conclusions: The average score of knowledge, attitude, and practice of dentists was average, and in general, the scores of specialists were higher than general dentists. Women had a higher attitude and practice score. People working in the private sector had a higher knowledge score. To increase the knowledge, attitude, and practice of general dentists, there is a need to hold training courses.
Mahnaz Shokri, Nabiollah Akbar Nataj Shub, Jamal Sadeghi, Arsalan Khanmoham Otaghsara,
Volume 27, Issue 1 (3-2024)
Abstract
Introduction: Several factors influence students' behaviors and develop motivation. Meanwhile, cognitive factors such as psychological capital play a more prominent role than others, and it is crucial to understand the factors that affect them. The current research purpose was to compare the effectiveness of mindfulness intervention and cognitive-behavioral game therapy on the psychological capital of female first-secondary school students in Amol City.
Methods: The research method was semi-experimental with a pre-test-post-test design with a control group. The statistical population included all the female students of the first secondary school of the seventh grade of the public schools of Amol City in the academic year of 2023. Among them, 45 people were selected by the available sampling method and were replaced randomly and by lottery in two experimental and control groups (30 individuals in the experimental group and 15 people in the control group). The people attending the first experimental group received mindfulness intervention (8 sessions) and two sessions per week for 60 minutes, and the second experimental group received cognitive-behavioral game therapy intervention (10 sessions) and two sessions per week for 30 minutes. The questionnaire used in this research included psychological capital. The study data was analyzed using the repeated measures analysis of variance (ANOVA) method.
Results: The findings showed that mindfulness and cognitive-behavioral game therapy affected the psychological capital of female students of the first secondary school in Amol City (P < 0.01). Also, mindfulness training on psychological capital was more effective than cognitive-behavioral game therapy (P < 0.01).
Conclusions: Although both methods of mindfulness training and play therapy improved students' psychological capital, the approach and method used in each are different. Mindfulness training focuses more on awareness and the presence of the mind in the present moment. At the same time, play therapy focuses more on strengthening psychological skills and abilities through interactive activities and games, which can better capitalize on increased psychological.
Fateme Tahsinin, Ali Heidarianpour, Elnaz Shokri,
Volume 28, Issue 1 (3-2025)
Abstract
Introduction: Being overweight is associated with increased cortisol secretion, along with heightened anxiety and depression. This study aimed to compare the effect of six weeks of concurrent moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) with HIIT training alone on body composition, plasma cortisol levels, and anxiety and depression levels in overweight male students.
Methods: In this semi-experimental research, 27 overweight students were selected and randomly divided into three groups 9: MICT+HIIT concurrent training, HIIT training, and a control group. The MICT )15 minutes of exercise per day at 60-65% of maximum heart rate with an additional 5 minutes every two weeks). The HIIT (3 minutes of running at 70-75% of maximum heart rate, followed by 3 minutes of active rest at 50% of maximum heart rate, with three repetitions in the first two weeks, four repetitions in the second two weeks, and five repetitions in the final two weeks).
Results: Both types of training protocols resulted in reductions in weight, body mass index, cholesterol, low-density lipoprotein, cortisol levels, anxiety, depression, and an increase in high-density lipoprotein (P < 0.05). There was no significant difference between the two types of exercise in terms of effectiveness on these variables; however, only peak oxygen consumption and triglycerides were associated with better changes in the MICT+HIIT group.
Conclusions: For overweight male adolescents aiming for weight loss and improved mental health, HIIT alone is sufficient, and adding MICT does not provide additional clinical benefits.
