Mohammad Husein Abnosi, Malek Soleimani Mehranjani, Hamidreza Momeni, Majid Mahdiyeh Najafabadi, Mozhgan Barati, Elham Shojafar,
Volume 14, Issue 1 (3-2011)
Abstract
Background: Molybdenum is an essential trace element for both animals and plants. Molybdenum (Mo), which functions as a cofactor for a limited number of enzymes including xanthine dehyrogenase, aldehyde oxidase, and sulfite oxidase in mammals, is believed to be an essential trace element in animal nutrition. The aim of this study is to evaluate the hepatoprotective potential of sodium molybdate against carbon tetrachloride (CCl4) induced liver damage. Materials and Methods: In an experimental study, adult male rats received daily oral administrations of different doses of sodium molybdate (0.05, 0.1, and 0.2 g/kg bw) along with intrapertioneal CCl4 (50% CCl4 in olive oil, 1 ml/kg bw) twice a week for 28 consecutive days. Results: Histopathological examinations in CCl4-treated rats showed extensive liver injuries characterized by extensive hepatocellular degeneration and necrosis, fat degeneration, and inflammatory cell infiltration while histopathological changes induced by CCl4 were significantly attenuated by sodium molybdate treatment. Conclusion: The results of this study suggest that sodium molybdate could protect liver against the CCl4-induced oxidative damage in rats, and this hepatoprotective effect might be contributed to the protection of liver by preventing the toxic chemical reactions which generate oxidative stress, lipid peroxidation, and molecular changes which ultimately lead to liver tissue necrosis.
Mahsa Soltani, Elham Shojafar, Ali Asghar Ghafarizadeh, Azam Moslemi, Maryam Baazm,
Volume 28, Issue 6 (1-2026)
Abstract
Introduction: Sperm cryopreservation is a crucial method for preserving fertility in patients with asthenoteratozoospermia. However, this process can lead to a reduction in sperm parameters due to the production of free radicals and damage to the cell membrane. Various substances are added to the cryopreservation medium to prevent cellular damage. In this study, platelet-rich plasma (PRP), which contains growth factors and bioactive molecules, was used to improve sperm parameters after freezing.
Methods: Semen samples were collected from 20 men diagnosed with asthenoteratozoospermia. The samples were randomly divided into five groups: control (no PRP), PRP50, PRP100, PRP200, and PRP400. Homologous PRP was prepared and added to the respective groups. The sperm samples were cryopreserved in liquid nitrogen. Twenty days after freezing, samples were thawed and subjected to a comprehensive evaluation of viability, motility, morphology, malondialdehyde (MDA) levels, and DNA fragmentation using specific assay kits. The findings were evaluated using one-way analysis of variance followed by Tukey's post hoc test.
Results: The results of this study demonstrated that cryopreservation led to a significant decrease in all sperm parameters in the control group. The addition of PRP at concentrations of 50 and 400 among the concentrations used resulted in a significant increase in total motility, progressive and non-progressive motility, sperm viability, and a decrease in immotile sperm, DNA fragmentation, and MDA levels compared to the control group (p<0.05).
Conclusions: Cryopreservation and subsequent thawing can have detrimental effects on the biological properties of sperm samples. Therefore, the dose-dependent addition of platelet-rich plasma as a cryoprotectant may offer a promising approach to mitigate the negative impacts of freezing on samples from men with asthenoteratozoospermia.
