Mohammad Shayestehpour, Mohammad Kazem Shahkarami, Abbas Shafyi, Mohammad Taqavian, Razieh Kamali Jamil, Fatemeh Esna-Ashari, Ashraf Mohammadi, Reza Shahbazi,
Volume 15, Issue 4 (September 2012)
Abstract
Background: Noticing the sensitivity of measles virus to temperature and light, maintaining its stability is highly important in live vaccines. The aim of the study is to evaluate the stability of measles vaccine produced by AIK-C strain. Materials and Methods: In this experimental study, three lyophilized vaccine vials were incubated at 37˚C for one week and their stability was evaluated via accelerated test. In addition, reconstituted vaccines were incubated at 4˚C, 25˚C, and 37˚C for 0, 4, 8, 12, 16 hours after reconstitution and their remaining infectious virus titer was measured using CCID50 method. Half-life of the reconstituted measles vaccine was evaluated according to linear regression analysis. Results: When the reconstituted vaccine was incubated at 4˚C, 25˚C, and 37˚C, the titer loss per hour was equal to 0.05, 0.1 and 0.2 Log10 CCID50, respectively. Also, the half-life of this vaccine at these temperatures was 5.31, 2.26, and 1.36 hours, respectively. Conclusion: The loss of potency for measles vaccine produced by AIK-C strain is 0.33 Log after storage at 37°C for one week, while the reported amounts for commercial vaccines such as Mevilin-L, Attenuvax, Edmonston-Zagreb, and Rimevax are 0.7, 0.7, 1 and 0.78, respectively. Lyophilized and reconstituted vaccine containing AIK-C strain is more stable in comparison with Edmonston B, Schwartz, Biken-CAM, and Leningrad strains. The stability of the reconstituted AIK-C strain vaccine is similar to Moraten strain at 37˚C.
Zohreh Karimi Taheri, Mohammad Hosein Aarabi, Ali Nazari Alam, Majid Nejati, Mohammad Shayestehpour, Hamid Reza Gilasi, Afshin Salehi, Mohammad Esmaeil Shahaboddin,
Volume 24, Issue 1 (April & May 2021)
Abstract
Background and Aim: Despite the anti-cancer and antimicrobial properties of licorice extract and lavender essential oil, some factors, such as low bioavailability and biodegradable, limit their therapeutic use. Using nanoparticles is a method to overcome these restrictions. This study aimed to investigate the anti-proliferative effects of nanoemulsion containing licorice extract and lavender essential oil on cancer cells; we also evaluated its antimicrobial properties in vitro.
Methods & Materials: In this experimental study, nanoemulsions, containing licorice extract and lavender essential oil were developed by the spontaneous emulsion method. The anti-proliferative effect of nanoemulsion was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetric method on two cell lines HepG2 and SK-MEL-3. To measure the antimicrobial effect of 4 standard strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Minimum Inhibitory Concentration (MIC) method was used.
Ethical Considerations: This study was approved by the Ethics Committee of Kashan University of Medical Sciences (Code: IR.KAUMS.MEDNT.REC.1396.106).
Results: The results of MTT test on HepG2 cells indicated that the concentrations of 630, 1250, and 2500 μg/mL nanoemulsions caused toxicity to the cell and led to the death of >50% of the cells (IC50=401μg/mL; P<0.05). Evaluating SK-MEL3 cells revealed that except for 75 μg of nanoemulsion, other concentrations induced death in >50% of the cells (IC50 = 82 μg/mL; P<0.05). In addition, nanoemulsions, with antimicrobial properties, were studied in 4 strains of bacteria; the highest antimicrobial properties were observed in Staphylococcus epidermidis.
Conclusion: Nanoemulsion containing licorice extract and lavender essential oil presents antimicrobial and antiproliferative effects on the two cell lines studied. The current study results indicated that the nano emulsification of lavender essential oil and licorice extract can enhance their biological impact; thus, they can be used as a drug formulation.
