Seyed Mahmoud Tabatabaei, Gholamreza Chalabianloo, Neda Seyedi,
Volume 20, Issue 10 (1-2018)
Abstract
Abstract
Background: The activation of inflammatory cascades reactions has been consistently demonstrated in the pathophysiology of Alzheimer’s disease (AD). Among several neuroinflammatory mechanisms, the tumor necrosis factor (TNF) signaling system has a central role in this process. The abnormal production of inflammatory factors may accompany the progression from mild cognitive impairment (MCI) to dementia. We aimed to examine serum levels of soluble TNF receptor (sTNFR1) in patients with MCI and AD as compared to cognitively unimpaired elderly subjects. We further aimed to investigate whether abnormal levels of these cytokines predict the progression from MCI to AD upon follow up.
Materials and Methods: We utilized cross-sectional determination of serum levels of sTNFR1 (ELISA method) in a test group comprising 150 older adults (30 AD, 60 MCI, and 60 healthy controls), and longitudinal reassessment of clinical status after12 months.
Results: At baseline, there were statistically significant differences in serum sTNFR1 between patients with MCI and AD and controls (p< 0.05). Also, patients with MCI who had more disorder in diagnostic functions and progressed to AD after one year, had significantly higher serum sTNFR1 levels as opposed to patients who retained the diagnosis of MCI upon follow up (p=0.03).
Conclusion: The results showed that abnormal activation of TNF signaling system, represented by increased expression of sTNFR1, is associated with a higher risk of progression from MCI to AD.
Seyed Hadi Seyedi, Rambod Khajei, Amir Rashid Lamir, Mohammad Reza Ramazan Poor, Jamshid Mehrzad,
Volume 23, Issue 4 (October & November 2020)
Abstract
Background and Aim: Coronary Artery Disease (CAD) is one of the leading causes of death and mortality in today's societies. Physical activity increases some of the influential factors for this disease. The purpose of this study was to investigate the effect of 8 weeks of aerobic and resistance training on endostatin in patients with Coronary Artery Bypass Graft (CABG).
Methods & Materials: The study participants were 24 male patients who were randomly divided into the experimental (n=12) and control (n=12) groups with Mean±SD age of 55.37±6.90 years, weight 75.45±5.87 kg, height 173.27±3.36 cm and body mass index of 25.11±1.55 kg/m2. The experimental group performed 8 weeks of aerobic and resistance training (3 sessions per week and 1.5 hours per session) based on the measurements, while the control group did not exercise during this period. To measure endostatin concentrations, blood samples were taken 48 hours before and 48 hours after the last training session while all subjects were fasting. Data were analyzed using the Shapiro-Wilk test to normalize the data and Student t-test in independent and correlated groups at the significant level of 0.05.
Ethical Considerations: This article was ethically approved by Azad University of Neyshabur (Ethics Code IR.IAU.NEYSHABUR.REC.1398.018) and with the Clinical Trial Code IRCT20191228045916N1 in the Iranian Registry of Clinical Trials.
Results: The present study showed that the aerobic and resistance training group significantly decreased endostatin concentrations (P=0.001) (t=1.672) compared with the control group.
Conclusion: Based on the findings, aerobic and resistance training decreases endostatin concentrations, known as an anti-angiogenic factor.
Andia Seyedi Moghaddam, Mahdieh Salimi, Najmeh Ranji, Hossein Mozdarani,
Volume 25, Issue 1 (April & May- 2022)
Abstract
Background and Aim MicroRNAs (miRNAs) are a class of small non-coding RNAs (17-25 nucleotides) that have been studied in many diseases. miRNAs studies in different cancers have shown that miRNAs may be considered oncogene or tumor suppressor. So far, many studies have shown that miR-17-5p and miR-93-5p are important regulatory molecules in some biological processes, such as cell proliferation, associated with cancer formation. This study aimed to investigate and compare the tissue and plasma expression levels of miR-17-5p and miR-93-5p in patients with ductal carcinoma breast cancer with the normal control group.
Methods & Materials The total RNA (including miRNA) was extracted from breast and plasma tissue samples of cancerous and normal samples. The RNA concentration and purity were confirmed using optical absorbance measurements. cDNA was synthesized, and the expression levels of miR-17-5p and miR-93-5p were assessed semi-quantitatively by SYBR Green-based real-time RT-PCR assay in plasma and breast tissues of ductal carcinoma breast cancer compared with the control normal samples with SNORD47 as internal normalizer. Data were statistically evaluated using GraphPad Prism 8.0.2.
Ethical Considerations This study was approved by the Ethics Committee of the institute (IRAN 52d/4922, 6.10.2016). All study individuals signed a consent form to use their clinical samples and personal data under the physician’s supervision.
Results The expression level of miR-17-5p showed significantly higher expression in tissues and plasma of the cancer group compared with the control group (P<0.0001). It was also significantly associated with tumor stage and lymph node, and ER (estrogen receptor) and PR (progesterone receptor) status (P<0.0001). While decreased expression of miR-93-5p in plasma and tumor tissues was shown to be significantly associated with tumor stage and lymph node involvement (P<0.0001).
Conclusion The data revealed that high expression of miR-17-5p and low expression of miR-93-5p in both plasma and breast tumor might be associated with poor prognosis in breast cancer. However, miR-17-5p, due to the greater change in expression and ease of plasma detection, may serve as a possible non-invasive biomarker for breast cancer’s poor prognosis. Further follow-up studies are required to confirm this finding.
