Background: The most important pathogen in nosocomial infections are microorganisms in the patient's body. 90 percent of nosocomial infections caused by bacteria. Medlar is an medicinal plant that its therapeutic effects has historically been emphasized. The aim of this study was to evaluate the antibacterial effect of methanolic and ethanolic leaf extract of medlar against bacteria isolated from hospital environment.
Materials and Methods: In this experimental study, the Nosocomial bacteria were obtained from Shahid Mostafa Khomeini hospital, Ilam, Iran. Soxhlet extraction method was used for medlar leaf extract. Disk diffusion method was used to study the effect of antimicrobial and broth microdilution method were used to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC).
Results: Two strains of Pseudomonas aeruginosa, three strain of Staphylococcus aureus and five strains of Escherichia coli were isolated from hospital. The results showed that the methanolic extract of Medlar leaf inhibited the growth of all strains of pseudomonas aeruginosa and four strain of Staphylococcus aureus and also inhibits the growth of all strains of Escherichia coli strains except E4 strain. The maximum antimicrobial activity was against E2 strain that zone diameter around it was 19/67 Millimeters. Quantities of minimum inhibitory concentration for all three strains P1, P2 and P3 and E2, E3, E5, S1, S2 and S3 strains was equals with 125 mg/ml.
Conclusion: Medlar leaf methanolic extract possesses significant antibacterial activity against bacteria causing nosocomial infections and so this extract can be considered in the control of infectious diseases.
