Showing 6 results for Saki
Maryam Delphi, Mahsa Bakhit, Mojtaba Tavakoli, Ali Nikkhah, Azadeh Saki,
Volume 16, Issue 1 (4-2013)
Abstract
Background: ASSR is the latest threshold detection technology in pediatric audiology. The aim of this study is to investigate the degree to which ASSR thresholds correlate with click-ABR thresholds.
Materials and Methods: In this cross-sectional study, 32 children ranging in age from birth to 7 years were evaluated by ASSR and ABR in moderate to severe and severe to profound hearing loss groups.
Results: The correlation coefficients for frequencies of 500, 1000, 2000, and 4000, mean of 2-4 and mean of 1-4 kHz were between 0.68- 0.90 in moderate to severe hearing loss and 0.79-0.96 in severe to profound hearing loss.
Conclusion: Strong correlations were found between ASSR and click-ABR thresholds in all frequencies. This correlation was greater for higher degrees of hearing loss.
Mojgan Mirghafourvand, Aziz Homayouni Rad, Sakineh Mohammad Alizadeh Charandabi, Kolsoum Shokri,
Volume 18, Issue 8 (11-2015)
Abstract
Background: Constipation is a functional gastrointestinal disorder and the use of probiotics is considered as a new solution in prevention and treatment of constipation. The aim of this study is to review the clinical evidence about probiotics effects in the prevention and treatment of constipation.
Materials and Methods: To review the accessible evidence about efficacy of probiotics in the prevention and treatment of constipation, the search of key words such as constipation, probiotic and yoghurt are conducted in databases Pubmed, Medlinplus and Cochran from 1980-2013 and of 49 articles about constipation, 16 yandomized controlled clinical trial articles and 3 pre-test and post-test randomized trials that investigated the effects of probiotics on constipation were included in this study.
Results: Probiotics produced lactic acid and fatty acids with short chains that decrease the colon pH and stimulate muscular contractions and intestinal peristalsis. Some of probiotics can increase the mucous discharge and change linked bile salts to free bile salts entering much water to colon and leads to soft defecation.
Conclusion: Considering studies, daily use of probiotics such as lactobacillus acidophilus and bifidobacteria lactic 105-1011 CFU/gr for at least 1-2 weeks can help to decrease constipation.
Parisa Tahmasebi, Seyed Reza Kazemi Nezhad , Mohammad Amin Tabatabaiefar, Javad Mohammadi Asl, Nader Saki,
Volume 19, Issue 6 (9-2016)
Abstract
Background: Hearing loss is a common sensory impairment in humans which half of its causes are genetic reasons. Genetic hearing loss can be divided into the two types of syndromic and non-syndromic, which 80% of non-syndromic cases is Autosomal Recessive Non-Syndromic Hearing Loss. The aim of the present research is to determine the contribution of DFNB2 locus (MYO7A gene) in causing an autosomal recessive hearing loss in the one group of the deaf families of Khuzestan province.
Materials and Methods: This study was conducted on 26 families with autosomal recessive hearing loss (with 4 patients) and negative for GJB2 mutations in Khuzestan province. 22 families suffered from ARNSHL and 4 families suffered from Usher syndrome. Linkage analysis was performed by using STR (Short Tandem Repeat) markers related to DFNB2 locus. Each family’s genotype was determined by PCR-PAGE method. Furthermore, haplotypes drawing and LOD score calculations were performed.
Results: From 26 families with hearing loss participating in this research, following genetic linkage analysis and haplotypes drawing, two families (7.7% of the families) showed linkage to DFNB2 locus. One family (4.5%) suffered from ARNSHL and another family suffered from Usher syndrome.
Conclusion: The results of the present research show that the contribution of DFNB2 locus in causing hearing loss in the population of Khuzestan province was similar to other studies conducted in Iran and this locus with other important loci should be considered to check in the hearing loss panel.
Mahtab Khosrofar, Mohammad Reza Pourreza, Samira Asgharzadeh, Parisa Tahmasebi, Elahe Ali Asgari, Reza Ghasemikhah, Nader Saki, Javad Mohammadi-Asl, Morteza Hashemzadeh Chaleshtori, Mohammad Amin Tabatabaiefar,
Volume 20, Issue 3 (6-2017)
Abstract
Abstract
Background: Hearing loss (HL) is the most common congenital defect in humans. One or two in thousand newborn babies have prelingual hearing loss. Autosomal recessive non-syndromic hearing loss (ARNSHL) is the most common form of hereditary deafness. Hearing loss is more common in the developing countries which is due to genetic and environmental (cultural -health factors) reasons. HL has a wide range of clinical demonstrations including: congenital or late onset, conductive or sensory-neural, syndromic or non-syndromic hearing loss. The goal of this project is to determine the portion of the DFNB21 (TECTA) in ARNSHL in families with negative GJB2 gene in Khuzestan province.
Materials and Methods: We studied 21 families with ARNSHL with at least 4 patients and negative for GJB2 mutations from Khuzestan province. Genetic linkage analysis was performed using STR markers linked to DFNB21 locus.
Results: Following genetic linkage analysis and haplotyping, out of 21 families with ARNSHL, one family showed linkage to the DFNB21 (TECTA) locus.
Conclusion: The results of this project confirm other studies in Iran and give insight into the most common loci causing ARNSHL in Iran which could be helpful in research and clinic.
Hiva Saki, Azam Haddadi, Mahmoud Shavandi,
Volume 21, Issue 7 (2-2019)
Abstract
Background and Aim: In recent years, Multidrug resistance has been increasing among Klebsiella isolates. The aim of this study was to survey existence of integrons and its relation with antibiotic resistance among clinical isolates of Klebsiella.
Materials and Methods: From Jun 2015 to May 2016, 129 Klebsiella isolates collected from Karaj hospitals and laboratories. Statistical population included 80.6% female and 19.4% male. Antimicrobial susceptibility was performed using Kirby-Bauer disk diffusion and ESBLs producer were screened. Integrons were detected using PCR.
Ethical Considerations: This study with research ethics code IR.IAU.K.REC.1396.16 has been approved by research ethics committee at Islamic Azad University of Karaj, Iran.
Findings: The highest and lowest percentage of sensivity were found to ofloxacin (89.1%) and amoxcicillin (6.2%), respectively. 82.9% of isolates were resistant to more than two antibiotics from different classes. Among 129 isolates, 19.3% of the isolates harbour integrons. Frequencies of MDR among integron-positive isolates were 100%. Also, 71.3% and 28.7% of isolates were ESBLs positive and negative respectively.
Conclusion: Results showed integron elements were prevalent among MDR isolates. Integron-associated resistance genes can be served as reservoirs of multi drug resistance within clinical isolates and presence of integron can be used as a marker to identify MDR isolates. Prevalence of ESBLs among clinical isolates of Klebsiella showed that antibiotics like ampicillin or amoxicillin-clavulanic acid are not effecvtive anymore in treatment of UTIs.
Ahmad Sahabzamani, Dr. Maryam Sadrnia, Dr. Majid Akbari, Dr. Sasan Saki,
Volume 25, Issue 3 (August & September 2022)
Abstract
Background and Aim The efflux pump in Pseudomonas aeruginosa inhibits the effect of ciprofloxacin by releasing quinolones out of the cell. It is important to find compounds to inactivate or inhibit its activity to continue using the antibiotics. The present study was done to investigate using sertraline as an efflux pump inhibitor in P. aeruginosa to reduce antibiotic resistance.
Methods & Materials P. aeruginosa strains were isolated from clinical sources and identified by routine microbiological methods. Resistance of the isolates to ciprofloxacin was evaluated by Kirby–Bauer test. Resistance breakdown was investigated by adding sertraline to the Moller Hinton agar medium and determining the zone of inhibition of ciprofloxacin. Minimum inhibitory concentration (MIC) by microplate dilution method and Minimum bactericidal concentration (MBC) by culture and MTT method were done for the isolates and ATCC 27853. The presence of the efflux pump was evaluated by the phenotypic method using sertraline and serial dilution method of the liquid medium in a microplate, on ciprofloxacin-resistant strains. The presence of the producing gene of this pump was determined by the genotyping method in resistant strains by performing PCR. The standard PAO1 strain of P. aeruginosa was used as a positive control.
Ethical Considerations This study was approved by the Ethics Committee of the Faculty of Medical Sciences of Islamic Azad University, Brojerd Branch (Code: IR.IAU.B.REC.1401.011).
Results Based on Kirby–Bauer test results, three strains were considered resistant to ciprofloxacin. MIC of drug-resistant strains was between 32 and 64 mg/ml and MBC was between 16 and 32 mg/ml. By performing electrophoresis on the PCR products, it was determined that the tested strains contained the mexA gene encoding the efflux pump. In the agar medium without sertraline, the zone of inhibition around the ciprofloxacin disc was zero, but after adding sertraline, the diameter of the halo increased to 25 mm. The minimum inhibitory concentration of ciprofloxacin in the isolates before adding 25 µg of sertraline was 128 µg/ml and after adding sertraline, it was 4 µg/ml.
Conclusion It was concluded that sertraline inhibited the efficiency of the efflux pump in resistant P. aeruginosa isolates and reduced ciprofloxacin resistance.
