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Abstract

Background: Pain is a protective process in the body. There are different pathways for pain control in the central nervous system. Descending pain control system is one of pathways. The periaqueductal gray (PAG) is a structure known for its role in pain transmission and modulation. The aim of this study is to investigate the percent of interaction between the left and right PAG in unilateral left foot induced pain.

Materials and Methods: In this study, 60 rats (280+30g) in six groups were used (3test groups and 3 controls groups). In test groups, 0.5 microliter lidocaine was injected in the left PAG, right PAG or both to make local anesthesia. In control groups, 0.5 microliter of normal saline were injected. After 15 minutes, 50 microliter of 2.5% of formalin were injected subcutaneously to right hind paw of rats and nociception was detected in every 15 seconds for one hour.

Results: The induction of unilateral pain (left hind paw) in rats, can affect not only the ipsilateral but also the contralateral PAG nucleus.

Conclusion: This study showed that the left and right PAG nuclei have significant role on unidirectional nociception in formalin test in rats. The contralateral PAG, however, has a minor effect on nociception.

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Background and Aim: Cancer cannot be explained only by genetic alterations but involves epigenetic processes. Modifying histones by acetylation plays a key role in epigenetic regulation of gene expression and is controlled by the balance between Histone Deacetylases (HDAC) and Histone Acetyltransferases (HAT). The HDACs expression and activity could be involved in several tumorigenesis mechanisms, so their inhibition induces cancer cell cycle arrest and migration.
Methods & Materials: Quisinostat is a novel promising second-generation HDAC inhibitor class of hydroxamic acid with high cellular potency towards classes I and II HDACs. Therefore, its low IC50 (<0.5nM) and bioavailability have been chosen to carry out our studies. Cancer cells were treated with Quiznos at nM200, and cell migration was measured by fluorescent microscopy.
Ethical Considerations: This study was the result of a preliminary study of Shiraz University (Code: 96GCU3M1293).
Results: The data showed that treatment of cancer cells with Quiznos significantly (P<0.05) reduced cell migration. DMSO did not affect reducing cell migration.
Conclusion: In this project try to explore the possible therapeutic application of this HDAC inhibitor against colon cancer. This study showed Quisinostat exerts broad-spectrum antiproliferative activity and migration.