Showing 3 results for Sahraei
Dr Malak Soleimani, Dr Hedayat Sahraei, Dr Mehr Angiz Sadooghi, Ms Parisa Maleki,
Volume 9, Issue 2 (6-2006)
Abstract
Introduction: Investigations has showed that prenatal exposure to Morphine causes drug dependency and behavioral complications in new born rats. In this study effect of prenatal Morphine on the development of basal ganglia in rat embryos is investigated. Materials and Methods: In this experimental study 36 female rats with body weight between 250-300 grams were selected. After crossing with male rats they were divided into six groups of 12days control-Morphine, 14days control-Morphine and 17days control-Morphine groups. Morphine groups received 0.01mg/ml Morphine through their drinking water until the 12, 14 and 17th day of pregnancy (20ml each rat). Then rats were anesthetized and embryos were taken out and fixed. Their body weight and crown-rump length were measured. Then 5 micrometers sections were provided and stained using H & E method which were then evaluated using mutic program. Results: Body weight and length of embryos were reduced significantly in the 12&14th day of Morphine group rats in compare to their controls. The significant reduction of Basal Ganglia thickness was also found in all Morphine groups compared to their controls. Conclusion: Results showed that prenatal Morphine exposure may cause impairment in change development of Basal Ganglia.
Saghar Saeedabadi, Mehrangiz Sdoughi, Hedayat Sahraei, Hosein Bahadoran, Javad Fahanik Babaiee, Sirous Jalili,
Volume 11, Issue 1 (3-2008)
Abstract
Introduction: Opioids abuse is a nationwide problem in our country. Regarding the potential of these drugs in crossing placenta they can cause many defects in fetuses of human and also animals. In the present study, the effects of maternal Morphine consumption on olfactory bulb development in rats have been investigated. Materials and Methods: Female Wistar rats (weight: 250-300g) were crossed with male rats and the zero time of pregnancy was detected. Existence of vaginal plug and also sperm in vaginal smear were indicators of mating and pregnancy. Experimental group received 0.05 mg/ml of Morphine in drinking water daily. On embryonic day 19, pregnant animals were anesthetized by Chloroform and embryos were taken out surgically. Weight of the embryos was determined by a digital balance and their length (Crown-Rump, Frontal-Occipital, Biparietal, Abdominal-Transfer and Anterior-Posterior diameters) were determined by a caliper. Then embryos were fixed in Formalin 10% and tissues were processed and sectioned and stained in Hematoxilin & Eosin and Bielschwosky staining methods. These sections were investigated for olfactory bulb development by MOTIC software and light Microscope. Data was analyzed using t-test. Results: The decrease in embryonic weight and their diameter for experiment group was significant. In microscopic study, the increase in thickness of mitral layer and the decrease in total number of Tufted, Mitral and Granular cells in experimental group were significant. Conclusion: This study showed that oral Morphine consumption leads to olfactory bulb defects.
Ali Arash Anoushirvani, Azam Ahmadi, Reza Aghabozorgi, Sara Khalili, Maryam Sahraei, Taha Fereydouni, Zoha Khademi,
Volume 21, Issue 2 (5-2018)
Abstract
Abstract
Background: Breast cancer is the most common cancer in women. It has been proven the association of cause of this disease with changes in several genes. One of the pathways associated with breast cancer is the folate reuptake pathway. The key enzyme of this pathway is coded by the TYMS gene. MicroRNAs control the expression of genes by binding to their regulatory regions. In this study, we evaluated changes in the regulatory region of TYMS gene with demographic characteristics (including the grade of cancer and metastasis) in breast cancer patients.
Materials and Methods: In this study, the regulatory region of TYMS gene was investigated using related bioinformatics software. After collecting cancerous samples and DNA extraction from blood samples of normal and patients, change in the miRNA binding region by digestion with NlaIII enzyme was assayed.
Results: Bioinformatics studies showed that the restriction site of some of the endonuclease enzymes in the 3'-UTR of the TYMS gene is related to the binding region of miRNAs, including Hsa-miR-433-3p. The results indicated the correctness of the genomic purification process, the PCR and enzymatic digestion reaction. In this study, in the regulatory region, CC homozygote, AC heterozygote and AA mutant homozygote variant had differences with control group (OR: 1.3465, %95 CI: 0.7275 to 2.4923, p<0.05). Also, the association of AA genotypes with metastasis and high grade of the patients was confirmed statistically.
Conclusion: Studies have shown that some of polymorphisms in the key genes involved in cancer are directly related to their diagnosis and treatment process, and given the importance of timely diagnosis of cancer, the achievement of diagnostic biomarkers in breast cancer in the early stages will be important. Probably, the nucleotide change at the site of the microRNA binding site could be used as a diagnostic biomarker for degree of tumor progression.
