Showing 11 results for Safi
Zahed Safi Khani, Mohammad Safikhani ,
Volume 2, Issue 7 (Summer 1999)
Abstract
The human vertebral in anatomical position have two curves thoracic and sacral with posterior curvature and two lumbar and cervical with anterior curvature. The vertebral column no lateral curves. Any curves in the both sides are called scoliosis. Presence of scoliosis is accompany with medical problems such as cardiopulmonary failure, lumbar pain asimetry and deformity. Present project is considered all of the pre-schools boys have done in ages between 11-15 years in every four educational districts of Ahwaz by school screening, 1033 cases was randomly put under. Consideration to determine the rate of scoliosis percentage measuring the length of hight, hands, feet and arm span, the relationship between theses figures and coliosis was determined, the rate of scoliosis in considering population was 1.36% in of all persons the difference who were studied between the length of hands was 5 to 12 mm. The difference between the hight and ram span with those who has scoliosis compare to normal people was considered to be more, the statistic examination was showed that the difference between the length of both hands and feet with the patients meaningful.
Safieh Sofian, Hossein Nadri Manesh, Abdolali Alizadeh,
Volume 11, Issue 4 (12-2008)
Abstract
Background: Aurein 1/2 is a 13-residue peptide with a vast antimicrobial and anticancer activity. Two- dimensional NMR spectroscopy of peptide solubilized in the 70% TFE (2, 2, 2-Trifluoroethanol) indicated an alpha-helical conformation. The mechanism of its action is not yet fully recognized. This study was designed to improve the antimicrobial activity and relationship between subsequence-activity in Aurein 1/2 and its analoges. Analogs of this peptide were designed and synthesized. Methods and Materials: The G1F3/RW and F3W analogs and retro - analog were synthesized with solide phase and purified via HPLC and lyophilized. These analogs were assayed by several methods: amino acid analysis, HPLC, and electrospray mass spectrometry. Then antimicrobial activity of the peptides was assessed by using the standard microdilution susceptibility test. Results: The data demonstrated that G1F3/RW analog had a higher activity and results of test figure of minimum inhibitory concentration for F3W analog had three levels. But the native, F3W analog and retro-analog was inactive. Conclusion: The higher activity of G1F3/RW in compare to F3W may be related to the positive charge of Arg that leading stronger interaction with the negative charges on the membrane surface. The result showed that reversed direction of aurein 1/2 significantly effects on activity of the peptide. It is also suggested inactivation of reto-analog amino acid type, position and size should be cautious for peptides designed as drug because it may be effect to control dimerization and maintenance of antimicrobial activity of the peptide.
Leila Hasanzadeh, Hamid Abtahi, Ehsanollah Ghaznavi-Rad , Safieh Soufian , Vahideh Farjadi ,
Volume 16, Issue 1 (4-2013)
Abstract
Background: Helicobacter pylori is the most common bacteria causing chronic infections worldwide. An important virulence factor of H. pylori is a vacuolating cytotoxin (VacA) that induces the formation of acidic vacuoles in cytoplasm and damage to epithelial cells. The aim of this study was to examine the antigenic properties of the recombinant VacA of H. pylori in infected sera of mice and human.
Materials and Methods: In this experimental study, the highly antigenic region of VacA gene (1233 bp) was detected by bioinformatics methods, and it was amplified by PCR method and cloned into the pET32a expression vector. After expression and purification of the target protein, its antigenicity was studied by Western Blotting using human sera infected with H. pylori and sera from immunized mice infected with purified recombinant VacA.
Results: PCR and sequencing results showed that the target gene was correctly cloned into the recombinant vector. Antibodies used in Western Blotting indicated the production and expression of the recombinant protein (65kDa) with concentration of 2.1 mg/ml.
Conclusion: Recombinant VacA protein has antigenic and immunogenic properties thus, it is a proper candidate for designing H. pylori vaccine and diagnostic kits
Hosein Heydari , Mehdi Shafiee Ardestani, Rezvan Zabihollahi, Seyed Mehdi Sadat , Shiva Irani , Seyed Nezamedin Hoseini, Safieh Amini, Seyed Davar Syadat, Mohammad Sadegh Khosravi, Alireza Azizi Saraji, Pouneh Rahimi, Mohammad Reza Aghasadeghi,
Volume 16, Issue 6 (9-2013)
Abstract
Background: Due to the lack of efficient anti-HIV vaccine, anti-HIV pharmaceuticals play an important role in controlling HIV infection. Also significant rise in drug resistance and drug toxicity has caused increased interest in finding new anti-HIV agents. In this study, a nano-sized version of lamivudine based on PEGylated chitosan was synthesized.
Materials and Methods: In this research, nanoparticles of chitosan were efficiently PEGylated for increasing their stability in water and then the anti-HIV drug, lamivudine, was loaded on these PEGylated nanoparticles. After purification and lyophilization of new synthesized nanoparticle, the raw materials and final product were sampled and FTIR, HNMR and CHN analyses were done.
Results: Results of HNMR spectroscopy showed that chitosan nanoparticle was successfully PEGylated. HNMR data confirmed FTIR results and indicated that lamivudine was conjugated on chitosan nanoparticle. In addition, CHN analysis data also confirmed both HNMR and FTIR data, and demonstrated that a high yield of chitosan nanoparticle PEGylation (approximately 97%) was done and illustrated a high capacity of lamivudine conjugation on nano-sized PEGylated chitosan (30% W/W chitosan).
Conclusion: In this study, lamivudine drug was successfully synthesized, based on PEGylated chitosan nanoparticle.
Vahideh Farjadi , Hamid Abtahi, Mohammad Reza Zolfaghari, Safieh Soufian, Leila Hasanzadeh,
Volume 16, Issue 7 (10-2013)
Abstract
Background: Helicobacter pylori (H. pylori) is a gram negative bacilli that causes the stomach and duodenum diseases in human. An important virulence factor of H. pylori is a CagA gene that increases of colonization it in stomach epithelial cells and lead to inflammation and peptic ulcers. The aim of the present study was to production of recombinant protein containing highly antigenic region of CagA in E. coli.
Materials and Methods: In this experimental study, the antigenic region (1245 base pair) of CagA gene was detected by bioinformatics methods, proliferated by PCR method, digested by BamHI and XhoI restriction enzymes and cloned into pET32a plasmid and was expressed in the E. coli BL21 (DE3) pLysS with induced by IPTG. The expressed protein was purified with Ni-NTA kit and its antigenicity was studied by western blotting method.
Results: Data showed the successful cloning and expression of the target gene. Recombinant CagA protein purified by Ni-NTA kit and dialysis with concentration of 1.5 mg/ml. In western blotting, the produced protein was interacted with infected human and mice sera.
Conclusion: Results indicated that recombinant CagA protein (65 KDa) maintains its antigenicity, so could be used for serological diagnosis of H. pylori diseases and production of vaccine.
Fatemeh Safi, Hormoz Haddad Larijani, Mehri Jamilian, Bahman Sadeghi,
Volume 16, Issue 9 (12-2013)
Abstract
Background: Today, ultrasonography has become the main tool used for the evaluation of fetal anomalies. The aim of this study was to evaluate fetal heart rate changes immediately after combined two- and 4-dimensional ultrasound.
Materials and Methods: This study was performed on 191 pregnant women aged 15 to 45 years old who were referred for 4-dimensional ultrasonography in summer 2013. Before the onset of 2-dimensional ultrasonography, baseline fetal heart rate was measured by ultrasound. At the same time, the maternal heart rate was recorded during one minute. Then, combined ultrasonography was performed and same parameters were recorded at the end.
Results: Maternal heart rate significantly decreased after combined ultrasonography (p=0.0001). Fetal heart rate did not differ before and after ultrasonography (p=0.693).
Conclusion: Four-dimensional ultrasonography has no effect on fetal heart as an indicator for evaluating the fetus temperature.
Nafise O Sadat Mirjamali Mehrabadi, Safieh Soufian, Hamid Abtahi,
Volume 17, Issue 4 (7-2014)
Abstract
Background: Streptococcus pyogenes produce extracellular hyaluronidase enzyme which is directly associated with the spreading of the organism during infection. Hyaluronidase enzyme is able to break hyaluronic acid or interstitial cement. This enzyme might be used in cancer treatment.The objective of the present study was to clone and express the nucleotide sequence of this enzyme which is involved in hyaluronidase enzymatic activity.
Materials and Methods: The enzymatic region of hyaluronidase gene was detected by bioinformatics methods. The polymerase chain reaction method was used to amplify the region. The amplified product was cloned into the expression vector pET32a. E. coli BL21 (DE3) pLYsS was transformed with recombinant plasmids. Then gene expression was induced by IPTG. The expressed protein was purified successfully via affinity chromatography by NiNTA kit. The integrity of the product was confirmed by western-blot analysis.
Results: The nucleotide sequence of amplified gene was consistent with the streptocuccal hyaluronidase gene. The concentration of recombinant protein calculated to 500 mg purified protein per liter. The enzymatic region of recombinant protein from Streptococcus pyogenes was recognized by all five patient’s sera with Streptococcus infection.
Conclusion: In general, it is possible to produce the enzymatic regions of the Streptococcus pyogenes hyaluronidase in Escherichia coli. The antigenic property of the produced protein is well retained. Considering the product's domestic demand and also low efficiency of production and pathogenicity of Streptococcus species, it is possible to produce it as recombinant product.
Safiyeh Abbasi, Behnam Zamanzad,
Volume 18, Issue 6 (9-2015)
Abstract
Background: Colonization with MRSA is no longer limited to hospitalized patients or persons with predisposing risk factors and at present there are several strains of community-acquired MRSA (CA-MRSA). The purpose of this study is to determine etA and etB genes in isolated Staphylococcus aureus strains of clinical samples from teaching hospital in Shahrekord in 2014.
Materials and Methods: In this descriptive study, 220 clinical samples were collected from teaching hospitals in Shahrekord. The microbiologic characteristics of isolates were determined using microbiologyic standard methods. The MRSA detection was carried out on oxacillin agar medium. The detection of virulence genes etA and etB was used by PCR. Inducible resistance to clindamycin was tested by "D-test".
Results: In 220 isolates, 110 detected as S. aureus and 13 as MRSA. Inducible clindamycin resistance was seen in 4 (3.5%) of the isolates. The frequency of genes etA, etB in studied strains was 7.6 and 15.3, respectively. Also, Inducible resistance to clindamycin was seen in four isolates(2%).
Conclusion: The results of this study confirme the presence of community- acquired strains in Shahrekord. The results of this study indicate the presence of genes etA and etB in the strains studied, transforming into the strains of Staphylococcus aureus resistant to methicillin obtained from hospital, the development and transfering these strains in the community.
Hamid Dalvand, Mehdi Rassafiani, Seyed Ali Hosseini, Seyyed Ali Samadi, Hamid Reza Khankeh,
Volume 18, Issue 12 (3-2016)
Abstract
Background: Mothering handling plays an important role in the daily life of children with cerebral palsy (CP) and their mothers, so that the greatest amount of handling of children with CP is done by the mothers at home. The study aimed to explore the process of mothering handling in the children with CP at home.
Materials and Methods: This qualitative study was conducted using grounded theory methodology in Tehran. The purposeful sampling followed by theoretical sampling. Selecting participants continued to achieve theoretical saturation. The number of participants was 26 including 15 caregivers, 3 children with cerebral palsy, 5 occupational therapists, 1 physiotherapist, and 3 rehabilitation manegers. The main source for gathering data was in-depth, semi-structured interviews. Data were analyzed by Corbin & Straus method (2008).
Results: Six categories/themes emerged from the data, including: motherly care, confronting with unusual co-occupation, confusion in roles, effort to special care, dynamic management of handling, and continuing care. The central category, confrontation with unusual co-occupation, represented the main theme of the research, and all major categories had been related to it.
Conclusion: The results of this research could provide broad perspective to rehabilitation therapists, managers and policy makers for understanding the needs, meeting challenges and concerns of caregivers and children with CP and designing a comprehensive plan for handling process of children with CP and helping them to be empowered for achieving the proper management and effective handling.
Azade Riyahi, Mehdi Rassafiani, Samira Yazdani, Rahmatollah Moradzadeh,
Volume 20, Issue 11 (2-2018)
Abstract
Abstract
Background: Human need for food is one of the inherent or physiological needs that, along with the ability to eat and drink, is the most important factor of health, survival and longevity. Children with cerebral palsy suffer from severe maladjustment in the nutritional mechanism and the creation of unsafe, even risky, conditions for life, due to deficiencies in the disease. Because of the importance of Eating and Drinking Ability Classification System (EDACS) in the research and treatment of children with cerebral palsy, the purpose of this study was to determine the psychometric properties of the Persian version of this system.
Materials and Methods: After the forward and backward translation procedures and investigation of face and content validity, test–retest reliability was assessed between parents and therapists using the intra-class correlation coefficient (ICC). 130 Children (3 to 20 years, mean age 52.35 months and standard deviation 33.96; 73 boys, 57 girls) with various types of CP were classified according to EDACS by both parents and therapists.
Results: The test–retest reliability was high and significant; the ICC related to parents and occupational therapists was 0.98, and the ICC related to speech therapists (0.99) was higher than 0.90 that both of them showed high correlation.
Conclusion: The Persian version of the EDACS is found to be valid and reliable, and is suggested to be appropriate for the assessment of eating and drinking ability in children with cerebral palsy.
Homa Yousefie, Anahita Khodabakhshi-Koolaee, Mohammad Reza Falsafinejad,
Volume 22, Issue 6 (February & March 2020)
Abstract
Background and Aim: Currently, the prevalence of chronic kidney disease has increased for a variety of reasons. Renal diseases, followed by dialysis and kidney transplantation, can greatly affect the People's physical and mental health. Since most of previous studies have examined the physical and often preoperative problems of kidney transplant patients and many of them have used quantitative methods, the present study aimed to investigate the psychological challenges of renal disease patients after Kidney transplantation.
Methods & Materials: The study population consisted of kidney transplant patients referred to the Nephrology Clinic of Labafinejad Hospitalin Tehran, Iran in 2019 aged 35-50 years and 1-5 years had passed since their transplantation. A semi-structured in-depth interview was done for collecting information. A purposive sampling and continued until data saturation and until no themes were available. In the end, 15 patients were interviewed. Data analysis was performed by Colaizzi’s method.
Ethical Considerations: This study with a code of IR.IAU.SRE.1398.022 was approved by the Research Ethics Committee at Islamic Azad University, Science and Research Branch in Tehran, Iran.
Results: The primary themes were divided in to 3 main themes including: Concerns and psychologically disturbing feelings, emotional and spiritual support of the family, and uncertainty of receiving specialized, financial and welfare support.
Conclusion: Patients with kidney disease after transplantation surgery have psychological challenges in addition to physical problems that are of great importance to mental health professionals. Studying these challenges can provide a deeper understanding of their psychological problems and adaptation after surgery.
