Showing 7 results for Saeedi
Azam Malek Hoseini, Zhila Abed Saeedi , Shaban Ali Alizadeh, Seyed Mohammad Kazem Naeeni,
Volume 17, Issue 12 (3-2015)
Abstract
Background: The type of dressing used has great effct on pain relief when dressing patients is changed. So this study is performed to compare the effect of Acticoat dressing and normal dressing on pain of burn patients during dressing change.
Materials and Methods: This clinical trial was done on 64 patients with second and third degrees burn wounds in Vali-asr hospital. The patients were randomly divided in two groups (receiver of the Acticoat) and control (the receiver of silver sulfadiazine cream 1%) for 15 days. Pain was examined during dressing change by using numerical evaluation of the degree of pain on the first, seventh and fifteenth days. Finally, the data was examined by using statistical tests, Chi-square, Mann-Whitney tests, RM ANOVA.
Results: The results showed that there was no significant differences between the groups in baseline pain intensity statistically (p=0.20). Pain intensity had statistically significant difference in the experimental group and control group in the seventh and fifteent days of the study (p>0.001) so that the experimental group experienced less pain.
Conclusion: Acticoat dressing is a simple and practical method in burn patients according to the present results of study which is preferred in terms of reducing pain during dressing change compared to the conventional method.
Fatemeh Toroghi, Farhad Mashayekhi, Vahid Montazeri, Hamid Saeedi Saedi,
Volume 19, Issue 3 (6-2016)
Abstract
Background: MMP9, as a member of the MMPs family, codes a protein that is able to provide suitable infrastructures for the migration of cancer cells and angiogenesis within tumor. The aim of this study was to investigate the relationship between -1562 C> T polymorphism in MMP9 promoter and progression of breast cancer in northwest of Iran.
Materials and Methods: In this case-control study, 187 females from northwest of Iran were involved. Polymorphism of interest was determined by PCR-RFLP method using enzyme PaeI and statistical analysis was done by Med Calc software.
Results: Distribution of CC genotype in cancer and control groups was,44 and 62.5, tespectively and distribution of CT genotype in cancer and control groups was 56 and 37.5, respectively, In the statistical analysis, χ2 and p value were respectively, 5.4 and 0.01. There is a significant association between this polymorphism and lymph nodes involvement and presence of tumor larger than 2cm3 (respectively p= 0.005 and p = 0.03). The results of this study showed -1562 C> T polymorphism in MMP9 promoter is associated with stage II and higher stages of breast cancer in our population. Furthermore, CT genotype may increase the risk of lymph node metastasis and presence of tumor larger than 2 cm3 (OR= 4).
Conclusion: According to the results of this study, MMP9 (-1562 C> T) polymorphism may be used as a biomarker for breast cancer prognosis. Although, to achieve more definitive results, it is necessary to examine a larger population.
Fatemeh Donyapoor, Mehdi Zeinoddini, Ali Reza Saeedinia,
Volume 19, Issue 5 (8-2016)
Abstract
Background: Immunotoxin (IT) is a directed toxin containing two distinct sections (immune and toxin parts) covalently bond using specific chemical or peptide linkers. The aim of this study is to produce a recombinant and hybrid protein containing diphtheria toxin (DT) and granulocyte colony stimulating factor (G-CSF).
Materials and Methods: According to the structure of the first commercial recombinant immunotoxin (Ontak, hybrid protein containing DT fused interlukine2), gene encoding of DT and G-CSF was amplified using specific primers and plasmid template of pET-IDZ3 (pET21 harboring the gene encoding ontak immunotoxine) and pET-GCSF (pET23 harboring G-CSF), respectively. The DT-GCSF fusion protein produced using soeing PCR and specific primers. Finally, pET-DT-GCSF construction prepared using subcloning of DT-GCSF into pET21a(+) and confirmed by sequencing, SDS-PAGE and western blot technique.
Results: Gene encoding of DT-GCSF inserted into NdeI/EcoRI site of pET21 and the construction of strain producing DT-GCSF recombinant immunotoxin was confirmed using customary methods.
Conclusion: The cytokine fusion protein, DT-GCSF, could be used for the inhibition of G-CSF receptor bearing cancer cells.
Nader Saeedi, Mohammadreza Rezvanfar, Mehdi Hadidi, Farvah Asgharizadeh Mahani, Mojtaba Ahmadlou,
Volume 19, Issue 6 (9-2016)
Abstract
Background: The renin-angiotensin system(RAS) has a major role in development of diabetic nephropathy and blocking of RAS by inhibitors and blocking of angiotesin receptors is standard treatment for preventing kidney disease and proteinuria. It is reported that VIT-D analogues are able to suppress renin exertion and improve proteinuria. The aim of this study is to evaluate the effect of VitD analogue (calitriol) on reducing proteinuria in patients with diabetic nephropathy.
Materials and Methods: In this clinical trial study, 132 eligible patients that had diabetic nephropathy and hadn’t vit D deficiency were selected. The patients were divided into two equal groups. First group received the combination of losartan 25mg twice daily and calcitriol 0.25mg and second group received losartan 25 mg twice daly alone for 3 months. The FBS, lipid profile, ESR-CRP BUN, Cr, HbA1c, Ca, P, and 24 hours urine protein were evaluated in all patients at beginning and end of study and the results were statistcally compared.
Results: The 24-hour urine protein in losartan and calitriol group was improved compared to losartan. This difference was statistically significant (p=0.003). As well as, in kidney function (BUN, Cr) in the losartan and calcitriol group compared to losartan alone was significantly improved(p<0.05).
Conclusion: Combination of calcitriol with angiotesin receptor blockers(ARBs) is more effective than ARBs alone in improvement of proteinuria and real function.
Hosna Rastegarpouyani, Seyed Masoud Hosseini, Seyed Reza Mohebbi, Pedram Azimzadeh, Shabnam Kazemian, Mahsa Saeedi Niasar, Afsaneh Sharifian, Mohammad Reza Zali,
Volume 20, Issue 2 (5-2017)
Abstract
Background: Parvovirus 4 (PARV4) was first discovered in 2005, in a hepatitis B virus–infected injecting drug user (IDU). To date, the best evidence about PARV4 transmission is parenteral roots and comes from IDU individuals. It seems that the prevalence of the virus in the normal population is very low. In this study, we investigated the prevalence of PARV4 virus among patients with chronic HCV infection compared with healthy controls and related risk factors among these groups.
Materials and Methods: A total of 206 patients, including 103 patients with chronic HCV infection and 103 healthy controls, were studied by use of nested-PCR and also real-time PCR techniques.
Results: AST enzyme levels with a mean of 40.45+34.84 and 18.58+5.9 in patients and healthy group respectively and the amount of enzyme ALT among patients with a mean of 40.45+35.75 and 21.50+11.35 in patients and healthy group respectively, were reported. Finally, after screening all DNA samples from patients and controls, we discovered that none of these people are infected with the PARV4 virus.
Conclusion: This study is the first to investigate the occurrence of PARV4 among HCV patients in Iran. The results show that, the virus is not important in Iranian population, even in patients with blood born infections such as HCV and further studies in other areas and various groups are required.
Ali Ganji, Amir Mohammad Saeedi, Ali Ghazavi, Ghasem Mosayebi,
Volume 22, Issue 4 (9-2019)
Abstract
Cancer is one of the leading causes of death worldwide. Thus, it is important to find newer, more selective, and more effective therapies for this disease. One of these methods that have attracted many researchers is using anticancer peptides regarding their specificity, lower side effects, and higher effectiveness on the cancer cells. One type of anticancer peptides is antimicrobial peptides. Although they have already been studied and introduced as potential agents to fight infectious diseases, only recently they have been used as a new way of cancer treatment. For decades, antimicrobial peptides have been considered a component of the native immune system; however, they can also be used as anticancer peptides due to their mechanisms and properties. This new therapeutic approach can provide a promising pathway for optimal cancer treatment with fewer side effects.
Dr Abolhasan Halvani, Dr Sareh Rafatmagham, Dr Dorsa Saeedi,
Volume 27, Issue 1 (3-2024)
Abstract
Introduction: In this study, the effective factors in pleural malignancy based on pleural fluid characteristics and the type of primary tumor in Shahid Sadouqhi and Shahid Rahmonun hospitals of Yazd from 2019 to 2021 were evaluated.
Methods: In this descriptive study, 98 patients with pleural effusion referred for thoracoscopy to the thoracic surgery department in Shahid Sadouqhi and Shahid Rahmonun hospitals in Yazd were studied from 2019 to 2021. The method of data collection in this research was file reading. Among the patients who underwent thoracoscopy during this period, only patients who had at least one study of pleural fluid thoracentesis and pleural fluid analysis in terms of cytology (White blood cells (WBC) and Red blood cells (RBC)), protein, albumin, Lactate Dehydrogenase (LDH), and pH and after reviewing the biopsy pathology report the pleura of these patients were thoracocentesis. Those who had reported cases of pleural malignancy were included in the study. The database used in this research was a simple questionnaire containing the studied variables.
Results: In the examination of the pleural fluid of patients with pleural malignancy, in terms of the WBC level, most of the cases were below 1000 units per cubic milliliter. In terms of the LDH level, most of the cases were more than 1000 units/liter. More than 90% of cases had lymphocytes, and the lowest amount was 62 % of lymphocytes. All cases had exudative pleural fluid, which was exudative mainly due to LDH level and then due to both protein and LDH levels at the same time.
Conclusions: According to the findings, in patients with pleural malignancy, pleural effusion is exudative in all cases, mainly due to the effect of LDH in the pleural fluid. On the other hand, the percentage of white blood cells in all Cases of predominant lymphocyte percentage were seen.
