Showing 32 results for Sadeghi
Abdolrahim Sadeghi , Hosein Mozdarani ,
Volume 2, Issue 7 (Summer 1999)
Abstract
This research is performed to assess clastogenic capacity of cimetidine(CT) against clastogenic effect induced by benzene in terms of micronuclei. For this purpose micronuclei assay method was used. In each experimental group m 5 Balb /C mices was used. 24 hours after i. p .injections slides was prepared from bone marrow and frequency of micronuclei in polychromatic erythrocytes (PCEs) and normochromatic erythrocytes (NCEs) was obtained. Results indicated that CT at therapeutic dose (20mg/kg. b. w) can be reduce genetical damages (micronuclei) due to benzene (1010mg/Kb. B. w) with a dose- related manner (p<0.01, = -0.82). Therefore , our results indicated that CT has anticlastogenic capacity. According to above results and relatively long term use of CT, we suggest using it in the cases in which someone exposed to clastogenic agents such as benzene, especially when someone also suffer from diseases such as peptic ulcer.
Abdolrahim Sadeghi, Hamidreza Rozati, Minou Golestani, Azadeh Moshtagh, Mozhgan Sadeghi , Zoreh Sari,
Volume 4, Issue 2 (Summer 2001)
Abstract
About 3-5 percent of population suffer from mental retardation (MR) mildly to severely. One of the most common causes of Mr is chromosomal aberrations. In this research, Chromosmal study was performed for 100 Mental Retarders (MRS) by G-banding Method. 100 MRS were suffering from downs syndromes: 21 cases with 21 free Trisomy, 1 case chromosomal mosaism and residual case had Robertsonian traslocation 14; 21 also chromosomal mosaism. Other 8 MRS had be structural aberrations in chromosmals such as inversion (3 cases), partial trisomy (1 case), ring 22 chromosme (1 case) and breakages in different chromosmes (3 cases). The results showed that chromosomal aberrations are frequent between Mental Retarderes and the most common chromosomal aberrations occure between MRS is 21 free trisomy.
Masoumeh Sadeghi, Norodin Moayedi, Hamid Reza Rooh Afza , Abdolmehdi Baghaie, Sedigheh Asgari, Pejhman Aghdak , Firozeh Sajadi ,
Volume 7, Issue 3 (Autumn 2004)
Abstract
Introduction: Diabetes is one of the most important problems in all countries. This study is designed to determine the prevalence of diabetes or glucose intolerance in central areas of Iran.
Materials and Methods: In a descriptive study in Arak, Isfahan and Najafabad cities, 12514 samples over 19 years old were selected by a multistage random sampling method. FBs and GTT were done. Lipid tests were also done in fasting. weight, height and blood pressure of samples were measured by standard methods. FBs>-126 or 2 hoursglucose >-200 was considered diabetes. Fasting glucose disturbance was considered with FBS <126 and glucose intolerance with 2 hours glucose >-140-200. Data was analyzed by SPSS software, t test, chi square and Fisher test.
Results: Of 12514 samples under investigation 51.1% were female. The overall diabetes incidence in urban and rural areas was 6.7% and 5.3% and in male and female 5.4% and 7.1% respectively .Mean of blood glucose was increased by age in both sexes. This was also increased in women of urban areas against women in rural parts. It was also found that glucose intolerance, known diabetes and newly diagnosed diabetes was increased by age. More than half of diabetes cases in all age groups were newly diagnosed. Blood pressure, age, BMI and abdominal obesity were higher in diabetics and those with glucose intolerance. These problems were more incident in women.
Conclusion: Regarding to the fact that diabetes is one of the major risk factors for noncommunitable disease and also its relative high incidence in central areas of Iran, it is necessary to have expanded programs for prevention and treatment diabetes family history.
Dr Effat Sadeghin, Dr Ali Heidarian-Poor, Dr Farhang Abed,
Volume 9, Issue 2 (6-2006)
Abstract
Introduction: Infertility is an emotional crisis with physical, economic and psychosocial challenges, because it interferes with all personal life aspects. This event is a sever tension of life and produces strong emotional shock in the infertile couple. This study is done with the aim of comparison the psychiatric problems of infertile men and women referring to infertility clinic of Hamadan Fatemyeh Hospital. Materials and Methods: This is a cross-sectional analytical study. Sample size was 400 infertile men and women which were selected by convenience sampling. Data was collected by a demographic questionnaire and SCL90, and analyzed through descriptive statistics and independent T test. Results: Our study indicates that infertile women experience mental problem significantly more than men. With p<0.05 infertile women in 9 categorize of SCL90 including depression, anxiety, phobia, psychosomatic, interpersonal sensitivity, hostility, paranoid ideation and psychotics are significantly different from infertile men. Conclusion: It seems women experience more stress in personal, social and sexual life and women are more sensitive to infertility phenomena that probably is due to cultural problems, mental susceptibility and/or special forms of social communication
Hamidreza Roohafza, Bahamn Roohafza, Masoumeh Sadeghi,
Volume 10, Issue 2 (6-2007)
Abstract
Introduction: Stress is a condition that causes tension, disturbance in body and mind, discomfort and dissatisfaction. There are differences between stresses in the form of great life events and small daily stressful events. Regarding extension and variation of stresses in different parts of country, this study aims to investigate the prevalence and severity of stresses in central areas of Iran. Materials and Methods: This is a cross-sectional analythical study carried out on 6000 people older than 19 years old, selected from urban and rural areas of Isfahan, Arak and Najaf Abad on 2002, as a part of evaluation of the Healthy Heart program. Sampling method was cluster random sampling. Data was collected using GHQ-12 and stressors' type and severity questionnaire. Statistical analysis was performed using Chi square. Results: Of the total subjects under study %50.6 were male, and %49.4 female, with a mean age of 40.47±15.68 and 40.25±15.15 years respectivly. The proportion of women with high GHQ score was higher than men, which was more pronounced in Arak than the two other cities. There were also higher stress levels in unmarried and urban people. (p0.05)The most prevalent stressor included economical, social and job-related issues and the most severe were death, family related and economical events. Conclusion: This study on stress as an indicator of lifestyle reveals high stress levels in the population, which warrants appropriate planning and community-based interventions, to improve lifestyle and reduce stress.
- Masoumeh Delaram, Zahra Sadeghiyan,
Volume 13, Issue 3 (9-2010)
Abstract
Background: Dysmenorrhea is seen in nearly 5% of women with regular menstruation. In this regard, different methods have been proposed for treatment of dysmenorrhea, and drugs with fewer side effects are preferred. Hence, this study was done to detect the effect of echinophora- platyloba on primary dysmenorrhea. Materials and Methods: In a single-blind clinical trial, 60 students with primary dysmenorrhea were evaluated at Shahr-e-Kord University of Medical Sciences. The students were randomly divided into two groups of 30 each: Echinophora-platyloba extract and placebo. Data collection was done through visual analogue scale for detecting the intensity of pain. At the end of first and second months after treatment, the intensity of pain was determined in the two groups and compared with that before the intervention. Eventually, the data were analyzed through Chi square, t-test and Mann-Whitney test. Results: The means of dysmenorrhea severity scores two months before administering the drug in echinophora-platyloba and placebo groups were 8.46±2.75 and 8.80±2.53, respectively. Here the difference was not significant however, after administering the drug, the mean scores in intervention and non-intervention groups were 3.41±1.12 and 7.82±2.60, respectively, which indicated a significant difference between the two groups (p<0.001) . Conclusion: Both echinophora-platyloba extract and the placebo could reduce the severity of dysmenorrhea during the treatment, yet the effect of echinophora-platyloba was much greater than the placebo. Thus, the use of echinophora-platyloba extract is suggested in treatment of dysmenorrhea.
Bahare Rahimi, Mana Shojapour, Abdorrahim Sadeghi, Ahmad Ali Pourbabayi,
Volume 15, Issue 3 (August 2012)
Abstract
Background: Pseudomonas aeruginosa is a human opportunistic pathogen which is considered one of the agents causing nosocamial infection. Recent studies have reported increased resistance of Pseudomonas aeruginosa to imipenem. The aim of this study was to determine resistance to antipseudomonal antibiotics including imipenem in Pseudomonas aeruginosa strains. Materials and Methods: In this cross-sectional study, 100 Pseudomonas aeruginosa strains obtained from clinical samples of patients in hospitals in Arak, Iran, were identified and isolated through microbiological methods, including Gram staining, oxidase test, Indol test, and oxidative-fermentative test. Then antibiotic susceptibility test was performed for imipenem, meropenem, gentamicin, amikacin, ciprofloxacin, and ceftazidime by disk diffusion method according to NCCLS (National Committee for Clinical Laboratory Standards) .Minimum inhibitory concentration (MIC) was done for determining imipenem-resistant strains Results: Antibiotic susceptibility test showed that resistance rates to imipenem, meropenem, gentamicin, amikacin, ciprofloxacin, and ceftazidime were 35%, 35%, 14%, 9%, 23% and 15%, respectively. Also, MIC test showed that 30 strains were resistant to imipenem, 27 to ceftazidime, 35 to cefepime, and 35 to ciprofloxacin. Conclusion: The results of this study indicated a high rate of antibiotics resistant of Pseudomonas aeroginosa strains to different antibiotic groups. Therefore, new and more effective methods should be found for controlling Pseudomonas infections and preventing the outbreak of its antibiotic-resistant strains.
Mana Shojapuor, Ghasem Mosayebi, Keyvan Ghasami, Ali Ghazavi, Abdolrahim Sadeghi,
Volume 15, Issue 4 (September 2012)
Abstract
Background: Multiple sclerosis (MS) is an autoimmune disease which demyelinates the central nervous system. Vitamin D, is a potential environmental factor which influences this disease. The majority of the biological activities of the polymorphism forms of vitamin D are done through its receptor gene (VDRG). The aim of this study was to examine the relationship between BsmI polymorphisms in VDRG and the incidence of MS. Materials and Methods: In this case-control study, the BsmI polymorphism in the VDRG was studied in 80 Iranian MS patients and 50 healthy controls of the same genetic background and age through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method Results: There was a significant difference in the frequency of BsmI VDRG polymorphism genotypes between MS patients and controls (P=0.023). Conclusion: This study indicated that the VDRG BsmI polymorphism is associated with MS in this population.
Hosein Heydari , Mehdi Shafiee Ardestani, Rezvan Zabihollahi, Seyed Mehdi Sadat , Shiva Irani , Seyed Nezamedin Hoseini, Safieh Amini, Seyed Davar Syadat, Mohammad Sadegh Khosravi, Alireza Azizi Saraji, Pouneh Rahimi, Mohammad Reza Aghasadeghi,
Volume 16, Issue 6 (9-2013)
Abstract
Background: Due to the lack of efficient anti-HIV vaccine, anti-HIV pharmaceuticals play an important role in controlling HIV infection. Also significant rise in drug resistance and drug toxicity has caused increased interest in finding new anti-HIV agents. In this study, a nano-sized version of lamivudine based on PEGylated chitosan was synthesized.
Materials and Methods: In this research, nanoparticles of chitosan were efficiently PEGylated for increasing their stability in water and then the anti-HIV drug, lamivudine, was loaded on these PEGylated nanoparticles. After purification and lyophilization of new synthesized nanoparticle, the raw materials and final product were sampled and FTIR, HNMR and CHN analyses were done.
Results: Results of HNMR spectroscopy showed that chitosan nanoparticle was successfully PEGylated. HNMR data confirmed FTIR results and indicated that lamivudine was conjugated on chitosan nanoparticle. In addition, CHN analysis data also confirmed both HNMR and FTIR data, and demonstrated that a high yield of chitosan nanoparticle PEGylation (approximately 97%) was done and illustrated a high capacity of lamivudine conjugation on nano-sized PEGylated chitosan (30% W/W chitosan).
Conclusion: In this study, lamivudine drug was successfully synthesized, based on PEGylated chitosan nanoparticle.
Fatemeh Safi, Hormoz Haddad Larijani, Mehri Jamilian, Bahman Sadeghi,
Volume 16, Issue 9 (12-2013)
Abstract
Background: Today, ultrasonography has become the main tool used for the evaluation of fetal anomalies. The aim of this study was to evaluate fetal heart rate changes immediately after combined two- and 4-dimensional ultrasound.
Materials and Methods: This study was performed on 191 pregnant women aged 15 to 45 years old who were referred for 4-dimensional ultrasonography in summer 2013. Before the onset of 2-dimensional ultrasonography, baseline fetal heart rate was measured by ultrasound. At the same time, the maternal heart rate was recorded during one minute. Then, combined ultrasonography was performed and same parameters were recorded at the end.
Results: Maternal heart rate significantly decreased after combined ultrasonography (p=0.0001). Fetal heart rate did not differ before and after ultrasonography (p=0.693).
Conclusion: Four-dimensional ultrasonography has no effect on fetal heart as an indicator for evaluating the fetus temperature.
Habib Soheili, Parsa Yousefi Chaijan, Anita Alaghmand, Bahman Sadeghi Saddeh, Amin Tavasoli, Mahdyieh Naziri,
Volume 17, Issue 1 (4-2014)
Abstract
Background: Allergic rhinitis affects 40% of general population and has an increasing prevalence. Sleep disturbance is an important problem in individuals with allergic rhinitis. Recent studies have shown that 68% of cases with perennial rhinitis and 48% with seasonal rhinitis have impaired sleep.
Materials and Methods: In this case-report study, 33 children with allergic rhinitis aging 6-18 years entered the study. Thirty five children without allergic rhinitis entered the study as control group. A questionnaire containing demographic data and types of sleep disorders filled for every child.
Results: Except respiratory disorder, there was no other significant difference between groups in any sleep disorder. Sixteen children (48.5%) in case group has respiratory disorder while none of control children were involved (P=0.0).
Conclusion: Nasal congestion is the main factor involved in sleep impairment in children with allergic rhinitis. Therefore, it seems that it is the first symptom to be treated.
Kavoos Shahsavari Nia, Farzad Rahmani, Hanieh Ebrahimi Bakhtavar, Ali Akbar Taher Aghdam, Elyar Sadeghi Hokmabadi, Armita Massood,
Volume 17, Issue 1 (4-2014)
Abstract
Background: Sudden visual loss is one of the emergent problems that it may be a sign of a life-threatening disease. It should be quickly evaluated and identified the treatable causes.
Case report: A young man without any history of certain disease was referred to the emergency department with complaints of sudden blindness. He was presented with fever, coriza symptoms and progressive blurring of vision that ultimately led to his visual loss. After carefully evaluation, finally suspected diagnosis of acute fulminant multiple sclerosis was made.
Discussion: Multiple sclerosis is a chronic inflammatory disease that is associated with acute attacks of neurological dysfunction. Some of these attacks are an emergent situation and need to early detection and treatment. There is a variant of Multiple sclerosis that called Acute Fulminant Multiple sclerosis which can cause sudden visual loss.
Vida Sadeghifard, Ahmad Ebrahimi, Mohamamd Reza Aghasadeghi, Seyed Mehdi Sadat,
Volume 17, Issue 2 (5-2014)
Abstract
Background: Schizophrenia is a widespread neurodegenerative disorder, which affects approximately 1% of the world population. It is a multifactorial and a highly heritable disease to which genetic factors contribute up to approximately 80%. Nowadays, multitude of genes have been discovered that relate to this disorder mostly by affecting the performance and levels of neurotransmitters in neural systems. Since PAI-1 is a considerable gene in the performance of neural systems, the present study dealt with the relationship between -675 4G/5G polymorphism in PAI-1 gene and schizophrenia among Iranian patients.
Materials and Methods: This case-control study was carried out on 106 blood samples collected from individuals suffering from schizophrenia and 122 healthy controls. DNA was extracted from the samples and the frequency of the polymorphisms was analyzed using ARMS-PCR method. Finally, the products were detected on 2% agarose gel electrophoresis.
Results: The analysis of the data for -675 4G/5G polymorphism showed that 17.9% of the patients and 1.6% of the controls were mutant homozygous and 65.1% of the patients and 45.9% of controls were heterozygous. Also, 17% of the patients and 52.5% of the controls were normal homozygous.
Conclusion: There was a significant relationship between PAI-1 4G/5G polymorphism and the incidence of schizophrenia. To the best of our knowledge, this is the first study in Iran that assesses the frequency of the polymorphism among Iranian patients. However, further studies with more samples are necessary.
Sedigheh Momenzadeh, Abdorrahim Sadeghi, Nasimeh Vatan Doust, Rsoul Salehi,
Volume 17, Issue 3 (6-2014)
Abstract
Background: Gene therapy is a recent promising treatment that effective gene transfer is considered as its most important step. Furthermore noninvasive method of transfer will be important to, when gene therapy is supposed to be applied. Administration of drugs in oral rout is more appreciated by patients. Loading and release rates are very important in targeting and effectiveness of transfer in all different methods that have been used for oral drug transfer. Here, we have studied packing of gene particles into two different enteric coats and compared these two coats in loading of entrapped materials and there release rate in vitro.
Materials and Methods: First, DNA was mixed with chitosan by coacervation technique and resulted polyplexes were coated using solvent evaporation technique. FTIR and two different pHs, less and more but near eudragit pKa, were used to evaluate formation of particles and their behavior.
Results: Formed particles have similar stability in low pH and their differences are trivial. Eudragit L100 release rate is really slower than L100-55 and gradual. Eudragit L100 shows better ability in loading rate.
Conclusion: According to two formed particles' behavior, eudragit L100-55 might be used in oral gene transfer targeting of initial part of small intestine and eudragit L100 might be used for wider surface of small intestine, from the initial to the end part, and colon.
Morteza Sadeghi, Zohreh Hojati, Kamran Ghaedi,
Volume 17, Issue 8 (11-2014)
Abstract
Background: Vascular endothelial growth factor (vegf) is one of the most important regulator of angiogenesis, there are some reports about the relation of VEGF over expression and progression of tumor in several cancers. The aim of this study is assay of four VEGF isoforms expression in breast cancer tumor samples.
Materials and Methods: 25 breast cancer tumor samples and 25 health samples were used in this study, mRNA was extracted from each sample and then cDNA was made. The expression of four isoforms VEGF121, VEGF165, VEGF183 and VEGF189 was measured by real time reverse transcription PCR (RT-PCR) and gel electrophoresis.
Results: Among the four isoforms, VEGF165 and VEGF 121 had maximum and VEGF 183 and VEGF 189 had minimum expression level in all samples. The total expression level of VEGF had a significant increase in tumor samples in comparison with the control samples (4/6, p<0.01).
Conclusion: There is a significant relation between the VEGF over expression and breast cancer tumor formation, which it can be used as a prognosis marker of breast cancer in future.
Zahra Hasanvand, Farideh Jalali Mashayekhi, Abdorrahim Sadeghi, Mohammad Reza Rezvanfar, Mohammad Taghi Goodarzi,
Volume 17, Issue 9 (12-2014)
Abstract
Background: Gestational diabetes mellitus (GDM) is defined as glucose intolerance with first dignosis during pregnancy. There is some evidence indicating that chemerin play a role in the development of GDM. In this study, for the first time, a possible association of rs17173608 polymorphism in the chemerin gene with the risk of GDM in Arak population was investigated.
Materials and Methods: In this case-control study, 120 GDM and 150 pregnant women with normal glucose tolerance were selected. GDM was confirmed by oral glucose tolerance according to the new IADPSG criteria. Genomic DNA was extracted from EDTA treated whole blood. The polymorphism of chemerin gene was determined using tetra-amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR).
Results: The genotype frequencies of TT, GT and GG at rs17173608 were respectively 81.7%, 17.5% and 0.8% in the GDM group and 73.3%, 25.3% and 1.3% in the control group. There were no statistical differences in genotype frequencies between case group and the control group. Also, allele frequencies in the GDM group (T 90.4% , G 9.6%), did not differ significantly from the control group ( T 96% ,G 14%). No association was found between genotype frequencies and FBS, 1h, 2 h and BMI.
Conclusion: The present study show that the rs17173608 polymorphism in the chemerin gene is not associated with the development of glucose intolerance and GDM in the studied population.
Kaveh Sadeghi, Shahla Shahsavandi, Mohammad Majid Ebrahimi, Homayoon Mahravani, Hadi Fazel,
Volume 17, Issue 11 (2-2015)
Abstract
Background: The outbreaks of new antigenic variants of influenza viruses in human populations have increased necessity the improvement of controlling programs. Influenza vaccines are formulated with adjuvant to enhance and direct the host immune responses. Currently, much effort is devoted to designing molecular adjutants. Hemokinin-1 (HK-1) activates T and B cells for proliferation, survival, differentiation into plasma cells, and antibody production. In this study, the effect of HK-1 as a molecular adjuvant for inducing humoral immune response against influenza virus was investigated.
Materials and Methods: The HK-1 coding sequence was cloned into pcDNA3.1 vector and used as adjuvant. Groups of mice were immunized with an inactivated influenza vaccine formulated with HK-1. The sera of vaccinated mice were collected prior to priming and boosting injections and at defined weeks, and analyzed with serological assays.
Results: The results showed that HK-1 was able to increase antibody titer against virus vaccine. The mice immunized with the adjuvanted vaccine produced higher antibody titers against influenza comparing to vaccine alone immunized group. Number of boosting had no effect on the enhancing of antibody titer.
Conclusion: These data revealed that HK-1 as a molecular adjuvant induces stronger humoral and memory responses against influenza immunization.
Somayyeh Saadatmand, Ahmad Hamta, Abdorrahim Sadeghi, Fathollah Mohaghghegh,
Volume 17, Issue 12 (3-2015)
Abstract
Background: Estrogen hormone regulates cell proliferation in breast tissue physiologically. Evidences show that changes in estrogen signaling pathways, including the receptor alpha (ER&alpha), happen during breast cancer progression. ER&alpha is expressed in most breast tumors and its association with the development of low-grade tumors has been demonstrated. Single nucleotide polymorphisms (SNPs) in genes may differ in susceptibility to cancer and result in different respond to treatment in different populations. The present study aimed investigated the association between single nucleotide polymorphisms (rs2234693: C/T) in gene ESR&alpha in patients with breast cancer.
Materials and Methods: In this case-control study 150 women with breast cancer and 142 healthy women without a family history of breast cancer were enrolled. DNA was extracted from blood samples. After primer design, technique of PCR-RFLP was used and samples were genotyped by acrylamide gel electrophoresis. Statistical analyzes were performed using SPSS version 20 and chi square test and Final findings were specified.
Results: TT and CT genotypes for ra2234693: C/T site compared with the CC had 5.5 and 1.5-fold increased risk respectively. Statistically significant differences were found between cases and controls for fibrocystic disease and age at menarche.
Conclusion: We not found an association between C/T polymorphism and breast cancer. But CC and TT genotypes of this polymorphism in estrogen receptor alpha gene related with breast cancer that are consistent with the findings of some other researchers.
Maryam Panahi, Syamak Rakei Isfahani, Mahmoud Amini, Bahman Sadeghi Sadeh,
Volume 18, Issue 9 (12-2015)
Abstract
Background: Despite advances in surgical techniques and risk management practices in recent years, wound dehiscence following abdominal laparotomy has still high prevalence. The purpose of this study is to evaluate the effect of retention sutures on wound dehiscence of emergent abdominal laparotomy.
Materials and Methods: In this randomized clinical trial study, 160 patients scheduled for emergency laparotomy for peritonitis or trauma were enrolled. Half of the patients randomly underwent retention suture and the other half underwent routine sutures. Finally, the amount of surgical wound dehiscence and complications were assessed.
Results: Three patients (3.7%) in retention group and 11 patients (13.7%) in the control group developed wound dehiscence which there was a significant difference between them (p=0.025). It was determined by multivariate logistic regression that wound dehiscence is correlated with hemodynamic status, type of trauma and injury to the abdominal organs, and sex. The incidence of wound dehiscence, early hemodynamic instability, penetrating abdominal trauma and damage to the intestines were higher in males.
Conclusion: In general, it seems that the preventive use of retention sutures in patients with multiple risk factors for postoperative wound dehiscence can be useful.
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Ahmad Hamta, Maryam Yousefi, Masood Fazeli Mosleh Abadi, Afsaneh Talaei, Abdorrahim Sadeghi,
Volume 18, Issue 9 (12-2015)
Abstract
Background: Thyroid nodules are common. 4-7% of adults have a palpable nodule and up to 50-70% of nodules are detected in high-resolution sonography. Thyroid nodules in women are 4 times greater than men and the rate of thyroid cancer in women is 3:1 compared to men, and is the sixth most common cancer in women. Epidemiological findings and experimental evidences show that sex hormones, especially estrogen, may have effect on this gland and its neoplasm. The aim of this study was to investigate the association between rs1256049 polymorphism in the estrogen receptor beta gene with thyroid nodular disease.
Materials and Methods: In this case-control study, 146 Patients with nodular thyroid and 151 health individuals were referred to Amiralmomenin hospital of Arak were recruited in study. Diagnosis is based on by ultrasonography and was confirmed by an endocrinologist. Genomic DNA was extracted from EDTA treated whole blood .The genotypes were determined using tetra-amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) and analyzed by statistical methods.
Results: The frequency of CC, TC and TT genotypes in case group 136(93.2%), 10(6.8%) and 0(0%) and in the control group 139(92.1%), 12(7.9%) and 0(0%) were obtained respectively. No statistically significant association (p=0.72) was observed between nodular thyroid disease and rs1256049 polymorphism.
Conclusion: Our findings showed no significant association between rs1256049 polymorphism and nodular thyroid disease. For best deduction, it is recommended that this study be done in other populations.
