Showing 9 results for Saadat
Mandana Yadollahi, Mostafa Saadat, Shapor Omidvari, Iraj Saadat,
Volume 4, Issue 4 (Winter 2001)
Abstract
Introduction: Glutatathion s-transferases (GST) are enzyme encoded by a multigene family and have important roles in detoxification of some strong carcinogens. Human GSTs are categorized into four groups. Namely π، μ، α، θ GSTM! Is a member of GST m previous studies revealed that absence of GSTM1 protein associates with increased risk of development of several malignancies.
Material and Method: In the current study, the relationship between GSTM1 genetic polymorphism and the susceptibility for being involved by gastric carcinoma was explored. Forthy patients with gastric carcinoma and 80 normal individuals (control group) were studied. GSTM1 genetic polymorphism between exons 5 and 6 was explored using a PCR technique. For each patient a questionair including gender, age, cigarette smoking, habit, and history of neoplasia in first-degree relatives was completed.
Results: The relative frequencies of null genotype in the control group and gastric cancer were 31.25 and 60% respectively. There was a statistically significant (x2=9.21; p<0.05) assessment between GSTM! Null genotype and development of gastric carcinoma.
Conclusion: Not mentioning the GSTM1 genotype, cigarette smoking and a positive family history had significant correlations with development of stomach malignancy.
Leyla Amjad, Ahmad Amjad, Fathalah Fallahian, Sara Saadatmand,
Volume 11, Issue 2 (6-2008)
Abstract
Introduction: Pollen grains are male gametophytes of flowering plants that with self interference in fertilization have an important role in plant fertilization, increasing fertilization and improving quality of products. Pollen grains are of important allergenic plants and 80-90% of allergens have plant origin. Achillea plant has medical usage and grows in different regions of the country. This research is done in order to acquire scientific information pertaining to pollen grains allergenicity in their development stages and comparing mature and immature pollen grains allergenicity. Materials and Methods: In this experimental study Achillea plant pollen grains in different developmental stages were collected around Isfahan city and samples were studied using light and electronic microscopy (SEM). Pollen extracts were prepared by incubating pollen grains in phosphate buffered saline, PH: 7.4. The allergenicity experiment was done on male Guinea pigs (Hartley strain, 350-500g weight, 4-6 week-old, Pausteur institute of Iran) and electrophoresis of proteins was done on 12% SDS- polyacrylamide gel. Data were analyzed using one way ANOVA and Duncan test. Results: Images of light and electronic microscopy showed pollens from ellipse-spherical type, with two colpate and echinate exine. The skin tests in Guinea pigs treated with pollen extracts indicated wheal with diameter larger than control group. In clinical tests, the numbers of eosinophils, neutrophils and IgE were increased in animals treated with pollen extract comparing control group. In SDS- PAGE protein profiles, 6 richly colored protein bands were seen in mature pollens in 14.4 to 66 KD and 5 slightly colored protein bands in immature pollens in 14.4 to 45 KD. Conclusion: This research shows changes of immature pollens` ellipse morphology to spherical form in mature pollens, partial increasing in accumulation and height of exine surface echins, changes in quality and quantity of immature and mature pollen grains and difference in their allergenic severity.
Mohammadbagher Salehi, Mojtaba Saadati, Babak Barati, Mahdi Saberi, Gholamreza Olaad, Aliasghar Rahimi,
Volume 14, Issue 6 (February-March 2012)
Abstract
Background: The major aim of this study was synthesis and assay of antimicrobial activity of peptide D28 and its new analogues derivatives as dimeric peptides.
Materials and Methods: Three antimicrobial peptides known as D28, Di-D28-Lys,Di-Cys-D28 including 20, 41, 42 residues were synthesized respectively. For peptide synthesis, solid phase peptide synthesis method using blocked amino acids with flourenyl methoxy carbonyl group and for peptide purification HPLC were used. Peptides compositions were confirmed by amino acid analysis and SDS-PAGE electrophoresis. Antimicrobial tests against Staphylococcus aureus and Pseudomonas aeruginosa were performed as disk and well diffusion on plate and by adding to liquid broth culture (Broth macrodilution) in different concentrations.
Results: Three required peptides (D28, Di-D28-Lys, Di-Cys-D28) successfully were synthesized. All three peptides were effective against S. aureus, but Di-Cys-D28 on the contrary to two other ones, showed no antimicrobial activity against P. aeruginosa. The inhibitory activity of Di-D28-Lys against P. aeruginosa was more than that of D28 peptide.
Conclusion: Improvement of antimicrobial peptides activity through dimerization depends on the methods of dimerization and the strain of bacterium. Di-D28-Lys peptide in comparison with D28 and Di-Cys-D28 showed wide range and more antimicrobial activity. Therefore, Di-D28-Lys peptide could be a suitable antibiotic candidate for future studies.
Sayed Mostafa Hosseini, Mojtaba Saadati, Mohammad Bagher Yakhchali, Bahar Nayeri Fasaei , Hoora Ahmadydanesh, Morteza Mirzaei, Kamal Baghery, Mokhtar Zare,
Volume 15, Issue 2 (June-July 2012)
Abstract
Background: Live attenuated Shigella vaccines have shown promise in inducing protective immune responses in human clinical trials. The aim of this study was to design and construct pDS132::∆icsA as a suicide plasmid for targeted deletion of a region of icsA gene in Shigella. Materials and Methods: In this experimental study, species and serotypes of Shigella isolated from diarrhea samples of children at Firozabadi and Milad Hospitals of Tehran were confirmed by using serological and PCR tests. Identification primers of icsA gene were designed and then cloned to the pGEM-5zf vector and sequenced. According to icsA restriction enzyme map, 1751 bp of icsA gene was deleted by HincП restriction enzyme and the ∆icsA was constructed successfully. The pGEM∆icsA vector was digested by use of SphI and SalI enzymes and was then cloned to a suicide vector (pSD132). Precision of the process was confirmed by phenotype and genotype tests. Results: The Shigella dysenteriae type 1 strain was verified by serological tests and PCR. Sequence of the icsA gene in the native strain was identical to the strains submitted in the gene-bank database. Since the pDS132::∆icsA contains 1484 bp derived from icsA gene, it can be used to disrupt icsA gene as a specific suicide vector. Conclusion: Application of suicide systems facilitated mutant construction in more specific and effective methods in comparison with the other primary techniques such as serial passage.
Hossien Honari, Mahdi Ghofrani Ivari, Mojtaba Saadati, Mohammad Ebrahim Minaei,
Volume 16, Issue 12 (3-2014)
Abstract
Background: Shigella dysenteriae one of the main causes of diarrhea in humans, but there is no vaccine against it. IpaD protein is one of the most important virulence factors in pathogenic shigella. The cloning N-terminal ipaD genes with ctB genes that have a role in adjuvant and carrier as recombinant vaccine can caused enhance the mucosal immune response.
Materials and Methods: Designing primers for genes ctB and ipaD were carried out based on the construction of gene cassettes, respectively. PCR reactions were performed to amplify the fragments and amplified fragments were cloned into pGEM-Teasy vector. Both the vector cut by restriction enzymes HindIII and XhoI and ipaD gene to gene ctB finally were Fusion. The ctB-ipaD gene cassette and expression vector pET28a(+) cuted by SalI and HindIII restriction enzymes. The cloning ctB-ipaD cassette was performed in the expression vector and expression of gene cassettes.
Results: In this study, the N-terminal ipaD as vaccine candidate antigen was genetically linked to the C terminal of ctB which has a carrier and adjuvant role. Fusing ctB-ipaD in the expression vector pET28a(+) is confirmed by sequencing, PCR and digested with restriction enzymes. The recombinant proteins produced is confirmed by SDS-PAGE and Western blot.
Conclusion: According to previous and similar studies, product cassette ctB-ipaD and expression its was expected. Is hoped to protein expression of this gene cassette and the production of antibodies could be achieved the candidate vaccine against Shigella.
Somayyeh Saadatmand, Ahmad Hamta, Abdorrahim Sadeghi, Fathollah Mohaghghegh,
Volume 17, Issue 12 (3-2015)
Abstract
Background: Estrogen hormone regulates cell proliferation in breast tissue physiologically. Evidences show that changes in estrogen signaling pathways, including the receptor alpha (ER&alpha), happen during breast cancer progression. ER&alpha is expressed in most breast tumors and its association with the development of low-grade tumors has been demonstrated. Single nucleotide polymorphisms (SNPs) in genes may differ in susceptibility to cancer and result in different respond to treatment in different populations. The present study aimed investigated the association between single nucleotide polymorphisms (rs2234693: C/T) in gene ESR&alpha in patients with breast cancer.
Materials and Methods: In this case-control study 150 women with breast cancer and 142 healthy women without a family history of breast cancer were enrolled. DNA was extracted from blood samples. After primer design, technique of PCR-RFLP was used and samples were genotyped by acrylamide gel electrophoresis. Statistical analyzes were performed using SPSS version 20 and chi square test and Final findings were specified.
Results: TT and CT genotypes for ra2234693: C/T site compared with the CC had 5.5 and 1.5-fold increased risk respectively. Statistically significant differences were found between cases and controls for fibrocystic disease and age at menarche.
Conclusion: We not found an association between C/T polymorphism and breast cancer. But CC and TT genotypes of this polymorphism in estrogen receptor alpha gene related with breast cancer that are consistent with the findings of some other researchers.
Mohammad Reza Hashemzadeh, Mojtaba Saadati, Mohamadreza Baghaban Eslaminejad, Reza Aflatoonian, Mokhtar Zarea,
Volume 18, Issue 3 (6-2015)
Abstract
Background: Shigella is the causative agent of human shigellosis and its lipopolysaccharide is detected by TLR4. TLR4 belongs to Toll-like receptors family and many immunological pathways are triggered when these receptors are stimulated. Many researches showed increasing in TLR4 expression in mesenchymal stem cells through lipopolysaccharide treatment. The main goal of this study is detecting the optimum lipopolysaccharide between shigella strains through stimulation of immune system for vaccine studies.
Materials and Methods: In this experimental study human bone marrow derived mesenchymal stem cells were treated with three distinct concentrations (0.1, 0.01, and 0.001) of shigella (S. flexneri, S. dysenteriae, S. sonnei) extract containing lipopolysaccharide. Then TLR4 expression in mRNA level was investigated by RT-PCR and Q-PCR. The cells treated with phosphate buffered saline have been considered as a control group.
Results: Expression of TLR4 was shown in all of case groups except treatment with concentration 0.001 of extracts from sonnei and dysenteriae and also control group. The variations in the expression of TLR4 was dose-dependent in all of case groups. The maximum expression of TLR4 related to treatment with extract from shigella flexneri strain and the minimum expression related to treatment with shigella sonnei extract. The use of lipopolysaccharide from E. coli as a positive control indicated that lipopolysaccharide in shigella extracts is responsible for the increased expression of TLR4.
Conclusion: The TLR4 expression level was increasesed by S. flexneri extract, so it could be recommended for increasing vaccine efficiency.
Mehrdad Nasrollahzadeh Sabet, Mohammad Foad Heidari, Mohammad Khanalipour, Saadat Allah Ghaffari, Milad Jafari Ashiani, Sajjad Biglari, Emran Esmaeilzadeh,
Volume 23, Issue 5 (December & January - Special Issue on COVID-19 2020)
Abstract
Background and Aim: Since late 2019, with the emergence of a new type of coronavirus that causes a new respiratory disease called COVID-19, there have been many concerns about the spread of this disease and how to deal with it. Due to the ability of the virus to be transmitted rapidly, diagnosing the infected individuals in the early stages for isolating them is critical. This study aims to evaluate the reliability of Computed Tomography (CT) scan in diagnosing COVID-19.
Methods & Materials: Participants were 212 patients admitted to hospital with confirmed diagnosis of COVID-19. Demographic information, medical history, symptoms, and the chest CT scan results were collected and analyzed. Finally, the power of CT scans in the diagnosis of this disease was compared with the Real-Time Polymerase Chain Reaction (RT-PCR) molecular test.
Ethical Considerations: This study received ethical approval from the ethics committee of AJA University of Medical Sciences (Code: IR.AJAUMS.REC.1399.091).
Results: The sensitivity of CT scan in the diagnosis of COVID-19 was relatively high, but its false-positive results were also high.
Conclusion: CT scan is a relatively sensitive method for diagnosing COVID-19, but caution should be made due to its high false-positive results which can lead to increased financial burden on the health system.
Abolfazl Kalantari, Hamid Rajabi, Pezhman Motamedi, Leila Poursaadat, Abbas Saremi,
Volume 25, Issue 6 (February & March 2023)
Abstract
Introduction: Increasing skeletal muscle contraction ability via increasing its neural stimulations, is one of the methods can be effective in sport performance improvement. The aim of the present study was to evaluate the effect of supramaximal isometric conditioning contractions on muscle neural excitation and performance indices during bench press exercise.
Methods: Current research is a semi experimental study performed on 8 athlete men. Age range of them was 19 – 23. Doing resistance trainings was a part of their exercise program at least 2 months before study onset. Bench press with Barbell was used in the protocol of these study. Electromyography was used in order to assay the neural excitation of main muscles which are activated during bench press. In addition, 1-RM test was done so as to assess the performance of these muscles. The protocol of this study was approved by the ethics committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1400.357). All subjects participated in this study voluntarily and they had no illness or injury at the start of the research.
Results: Neural excitation and performance of the muscles increased significantly during strength bench press, following conditioning contractions. This research was reviewed in Islamic Azad University - Arak Unit and approved with the ethics code IR.IAU.ARAK.REC.1401.096. Informed consent was obtained from the participants and they were assured that their information would be confidential
Conclusions: Doing supramaximal isometric conditioning contractions prior to doing strength bench press with barbell, increased neural excitation and performance indices of main muscles activated in this exercise.
