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Background: Otoacoustic emissions (OAEs) are faint sounds generated within the inner ear that can be regarded as a sensitive tool for reflecting inner ear status. There are various factors that may influence OAE recording. One such factor that has not been extensively examined to date is body position. The aim of this study was to examine the effect of body position on the OAE responses of adults with normal hearing. Materials and Methods: In an analytic cross-sectional study, 26 normal hearing adults (18-25 years old) were evaluated. Transient OAEs (TEOAEs) were recorded in five different positions: supine, sitting, side-lying, 30°-Up, and 30°-down to evaluate their inner ear function. Data were analyzed using SPSS software 17. Results: Mean TEOAE amplitude values were significantly higher in the side-lying position compared to other positions (P=0.01). The most significant TEOAE amplitude differences were observed at the high frequencies (3000 Hz and 4000 Hz) in side-lying position orientation compared to low and medium frequencies. Conclusion: The results from the present study revealed that body position has a remarkable effect on inner ear function, and this effect is more obvious at high frequencies.