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Background: Noticing the practical significance of stem cells, this study was conducted to culture and screen bone marrow mesenchymal stem cells derived from Raf and Hilline chicken strains and investigate the effect of age and race on the morphology and differentiation of the generated cells. Materials and Methods: In this fundamental study, bone marrow cells from 3 to 25 day-old Raf and Hiline chicken strains were cultured in low glucose DMEM, 10% BFS. Then third passage bone marrow cells of the two strains were compared in terms of morphology, differentiation to bone, cartilage, and adiposity. Data were analyzed through SPSS software. Results: In culturing Raf chicken derived bone marrow cells, in contrast to Hiline chicken strain, colonization took place and they almost had a better fibroblastic morphology. The results indicated higher yields of differentiation to bone, cartilage, and adipose tissues in Raf chicken derived bone marrow cells than Hiline chicken. These differences were statistically significant. Also, 15 days was the most suitable age for screening the mesenchymal stem cells of chicken. Conclusion: Screening and proliferation of mesenchymal stem cells from 15-day old Raf chicken bone marrow cells are good resources for differentiation and purification of chicken bone marrow mesenchymal stem cells.

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Background: Wound healing is a complex process that is impaired in diabetic patients due to several factors. So far, the positive effects of mesenchymal stem cells secretions in wound healing process have been reported. In this study, we investigated the effect of human mesenchymal stem cells Conditioned media on expression of effective factors involved in wound healing.

Materials and Methods: 27 rats were divided into 5 groups: no wound control, normal control, diabetic control, diabetic placebo and diabetic experimental. Diabetes was induced by Alloxan. A wound was created on the back of the rats. Then, the conditioned medium was prepared from mesenchymal stem cells. Diabetic experimental rats received 200 microliter of conditioned medium intravenously. The wounds were sampled and expression of KGF and TGF-&beta1 genes was examined by RT-PCR on days four and seven after wounding.

Results: In the diabetic experimental group, expression of KGF gene at fourth and seventh days had been non-significantly increased in comparison to diabetic control group. While, expression of TGF-&beta1 gene in diabetic experimental group compared to diabetic control group had been significantly (p<0.05) increased on fourth day, and non-significantly increased on seventh day.

Conclusion: It seems that using the conditioned medium derived from human mesenchymal stem cells positively affects the expression of trophic and inflammatory factors involved in diabetic skin wound healing.