Tahere Azimi, Malihe Bagheri, Mahdi Pariyan, Behzad Khansarinejad, Ashraf Zamani, Mahdieh Mondanizadeh,
Volume 23, Issue 3 (August & September 2020)
Abstract
Background and Aim: Cervical Cancer (CC) is the third most common malignancy in the women, the main cause of which is human papillomavirus (HPV). Both E6 and E7 oncogenes of the virus play an important role in its tumorigenesis. Today, methods available for screening CC are not capable of detecting the disease at an early stage. Therefore, it is important to identify new biomarkers for early detection of this cancer. For this purpose, in the present study, miRNAs targeting the two oncogenes E6 and E7 of human papillomavirus (types 16 and 18) were studied in CC by bioinformatics.
Methods & Materials: First, using the NCBI database, the E6 and E7 gene sequences were obtained for both human papillomavirus types 16 and 18. Then, using the miRBase and RNA22 bioinformatics databases, the most appropriate targeting miRNAs for these genes were selected.
Ethical Considerations: This study was approved by Ethics Committee of Arak University of Medical Sciences.
Results: Based on the P obtained from bioinformatics databases, miRNA including miR-92a-5p (P=7.51e-2), miR-195-3p (P=2.24e-1), miR-34a-5p (P=2.73e-1) and miR-155-5p (P=4.95e-2) were introduced for the two genes E6 and E7.
Conclusion: Results from bioinformatics studies revealed that of the four miRNAs identified, miR-155-5p and miR-92a-5p are probably the targeting miRNAs specific for the E6 and E7 genes, respectively. Therefore, it seems that these miRNAs can be a suitable candidate for in vitro studies in CC patients.
Pariyan Azad Derakht, Mohammad Fathi, Dr Raziyeh Rezaei, Raziyeh Rezaei,
Volume 27, Issue 5 (12-2024)
Abstract
Introduction: Physical activity positively influences various physiological factors, laying the groundwork for beneficial changes. Myostatin and follistatin are among these factors, whose levels are influenced by gender and physical activity. The present study was conducted with the aim of investigating the effect of traditional resistance training (RT) and high functional training (HIFT) on the serum levels of myostatin and follistatin in young women.
Methods: In this semi-experimental study, 30 young women were selected and divided into three groups: intense functional training, traditional resistance training, and control. Blood was drawn 24 hours before and 48 hours after the end of the interventions to measure the serum levels of myostatin and follistatin. The exercise protocols were performed for eight weeks, 3 days a week and 60 minutes a day. Ultimately, the collected data were analyzed using the ANOVA statistical method, with a significance level set at P < 0.05.
Results: The results showed that follistatin level was significantly higher in the RT and HIFT groups than in the control group (P = 0.001). Also, the amount of follistatin in the HIFT group was considerably higher than the RT group (P = 0.01). The results showed that the level of myostatin in the RT and HIFT groups was significantly lower than the control group (P = 0.001). Also, the amount of this factor in the HIFT group was substantially lower than in the RT group (P = 0.01).
Conclusions: In general, the findings of the present study show that performing intense resistance and functional exercises causes a favorable change in the expression of physiological factors related to muscle growth in women. It seems that the effect of intense functional exercises is greater than resistance exercises.
