Showing 5 results for Parivar
Dr Javad Baharara, Dr Kazem Parivar, Dr Shahrbanou Oryan, Dr Alireza Ashraf,
Volume 9, Issue 2 (6-2006)
Abstract
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Introduction: The increasing use of EMF (electromagnetic field) generating apparatus (refrigerators, computers, TV, etc) caused an increasing interest in investigations of its adverse effects on human health. This study is done to investigate the effects of EFM on Balb/c mice.
Materials and Methods: This is an experimental study in which at first a circuit generating low frequency electromagnetic field (50 Hz, 15G) was designed. Then adult virgin female mice were placed in coil and exposed to 15 gauss electromagnetic field for 4 day and 6 hour per day. Then their blood was examined to assay the level of hormones (FSH, LH, estradiol, progesterone). Also ovary and uterus sections were studied with light & electronic microscope.
Results : Results showed that the weight and size of ovary was not significantly affected in females exposed to the low frequency electromagnetic field and their offspring. Our results also showed that the number of ovary follicles were significantly affected in exposed females (p<0.05). Also the study of micrographs showed hetrochromatinated oocytes and follicular cells and increasing polysomes, accumulation of mitochondria and cleft nucleus. Decreasing amount of FSH, LH and 50% decrease in couplation rate was also seen as compared with the control group.
Conclusion: Results of this study is indicator of EFM effects on gonads, structure and endocrine system and decreases fertility.
Saeedeh Zafar Balanejad, Kazem Parivar, Javad Baharara, Homa Mohseni Koochesfahani,
Volume 12, Issue 1 (5-2009)
Abstract
Background: Angiogenesis is a complex process that occurs in many physiologic and pathologic conditions such as invasion and metastasis of tumors. Therefore, it is the target of many clinical treatments. Rapamycin is one of the immune system inhibitor drugs that recently has been used for controlling different types of cancer. In this study, the effect of Rapamycin on angiogenesis in chicks' chorioalantoic membrane was investigated. Methods and Materials: In this experimental study, we used 42 Ross fertilized eggs that were divided into 3 random groups: control, sham-exposed (treated by Dimethyle sulfoxide-DMSO- ) and treated with Rapamycin. In 2th day, a window was opened on eggs in the sterile condition. Later, in 8th day, a gelatin sponge appeared on chorioalantoic membrane and was soaked with 5 μl Rapamycin in treatment group and 5 μl DMSO in the sham-exposed group. In 12th day, CAMs were examined and photographed by Research Photostereomicroscope in all cases. The numbers and lengths of vessels around the sponges were measured and compared with each other by T-Test (p<0.05). Results: The mean of number (42 ±7.26 ) and length (57.25±5.05 cm ) for vessels in the control group and mean of number (42.93±8.37 ) and length (55.66±10.44 cm) in sham-exposed group was'nt any significant differences. There was a significant decrease in mean number (29.36±5.28) and length (44.55±10.22) of vessels in Rapamycin with control group. Conclusion: It seems Rapamycin has an inhibitory effect on angiogenesis in chicks' chorioalantoic membrane. It decreases the number and length of vessels around treated area
Javad Baharara, Kazem Parivar, Mojgan Madadii,
Volume 12, Issue 3 (10-2009)
Abstract
Abstract Background: Vitamin A is an important messager molecule for differentiation setting, cells proliferation and morphogenesis. In this research, an effect of vitamin A on limb bud development of Balb/C mouse was determined. Materials and Methods: In this experimental study, 10 female pregnant mice were divided to control and experimental groups. Control mice were maintained in natural situation and experimental mice were received vitamin A 15000IU/kg intraperitoneal injection at gestational day 10.5. Control and experimental mice were dissected in day 15.5 of gestation and after a morphology study their embryos were prepared for histological studies with microscope and were stained by Hematoxylin & Eosin method. Results: Comparison of crown- rump length, fore limb width, length of zone 1 (finger and palm) and zone 2 (wrist) of fore limb and total length of hind limb in experimental embryos with control group didn’t have significant difference in means. But, mean of embryos weights and length of total fore limb and length zone 3 (arm and forearm) of experimental embryos fore limb were more then control (p<0.001). Also, in comparison mean of hind limb width of experimental to contol embryos, increase was observed (p<0.006). But number and size of chondrocyte in 4 zones of fore and hind limb in experimental group didn’t have significant difference to control group (p>0.05). Conclusion: Concentration of 15000IU/kg vitamin A has progressive effects on the fetuses’ weight and fore limb bud development of Balb/C mouse.
Javad Baharara, Kazem Parivar, Alireza Ashraf, Roya Rostami,
Volume 12, Issue 4 (2-2010)
Abstract
Background: Noticing the daily advancement of the role of electromagnetic field generators in human life, examination of the biologic impacts of these waves has come to be extensively appreciated by researchers. During pregnancy, vitamin A is extremely essential for the development of fetus and its lack, insufficiency or excess can result in embryonic malformations. This study investigated the synergetic effects of vitamin A and extremely low frequency electromagnetic fields on the development of embryo skin in Balb/C mice. Materials and Methods: This study was experimental in nature. Eighteen pregnant mice were divided into control, sham-exposed and experimental groups. The mice in the experimental group were given an intra-peritoneal injection of 15000 IU/kg vitamin A on the 10.5th day of gestation and were, then, exposed to a 50Hz electromagnetic field 4 hours a day from 10 through 12th day of gestation. The animals were dissected and investigated morphologically and histologically on 17.5th day of gestation. Results: The results showed an increase in the mean weight of fetuses in the experimental group in comparison to the sham- exposed group (p<0.05). Also, Crown-Rump of fetuses in the experimental group increased in comparison to those in the sham- exposed group (p<0.001). Epidermis thickness increased significantly in experimental group in comparison to sham- exposed (p<0.05), while the average number of basal, spinous cells and hair follicles decreased significantly in the experimental group compared to the sham- exposed (p<0.001). Conclusion: The findings indicate that vitamin A usage and synergetic exposure to low frequency electromagnetic fields (50Hz) profoundly affect the development and growth of skin epidermis in the fetus of Balb/C mouse.
Pooran Badkoobeh Hezaveh, Kazem Parivar, Seyed Mehdi Kalantar, Alireza Salabat, Seyed Davood Hosseini,
Volume 16, Issue 1 (4-2013)
Abstract
Background: Doxorubicin (DOX) is a widely used anticancer drug, but its use in clinical chemotherapy is limited due to its side effects, including testicular toxicity. The aim of this study was to investigate the protective effect of nano-zinc oxide (nZnO) on DOX-induced reproductive toxicity in male rats.
Materials and Methods: In this experimental study, adult male Wistar rats were randomly divided into four groups, including one control and three experimental groups. They received saline, DOX (6 mg/kg), nZnO (5 mg/kg), and nZnO followed by DOX (i.p), respectively. Treatment was performed for 3 days. After 28 days, post-administration histological changes and reproductive indices were studied.
Results: Administration of DOX induced a significant reduction in seminiferous tubules diameter and thickness of germinal epithelium. Also, fertility and fecundity indices, number of litters and epididymal sperm concentration, decreased, whereas degenerated Leydig cells and deformed Sertoli cells increased.
Conclusion: Coadministration of nZnO significantly improved DOX-induced changes. These findings show the protective role of nZnO in DOX-induced reproductive toxicity.
