Mokhtar Nosrati, Mandana Behbahani,
Volume 18, Issue 3 (6-2015)
Abstract
Background: Because of the reported high ability of virulence and medicinal resistance of HIV-1 virus during the last decades, many investigations have been performed concerning discovery and the introduction of anti-HIV-1 drugs. The results of numerous researches have shown that drugs and protease inhibitory compounds mainly containing plant derivatives specially terpenoids may control HIV-1 infection very effectively. The aim of this research is the bioinformatical study of HIV-1 protease inhibition by standard drugs and triterpenoides from plant and mushroom.
Materials and Methods: This is a descriptive-analytic study. In the present study , the structure of drugs, triterpene comounds, and HIV-1 protease enzyme was received from the databases such as Chem Spider, PubChem, Human Metabolome Database (HMDB), and Protein Data Bank (PDB). After that, molecular docking was performed by iGRMDOCK 2.1 software
Results: The results confirmed that the interactions of the triterpene compounds like the standard drugs were in three safeguarded and catalytic areas including central domain, flap and carboxylic terminal domain specially amino acids Asp25, Asp27, Ala28, Asp29 and Asp30 in active sites of HIV-1 protease. Also, The study of the interactions of these areas showed that there is a direct correlation between the strength of the interactions and IC50 values of these compounds.
Conclusion: Finally, with due attention to the high effectiveness and the proprietary function of triterpenoids, we can conclude that these compounds may be considered as effectire HIV-1 antiprotease drugs.
Mokhtar Nosrati, Mandana Behbahani,
Volume 18, Issue 4 (7-2015)
Abstract
Background: Medicinal plants are primery source of many drugs to cure different diseases. The genus Prangos, (Umbelliferae family) consists of several medicinal plants that their desirable dffects have been approved. The aim of this study is to investigate the effectiveness of methanol extract in different parts of prangos ferulacea and prangos acaualis on human lymphocytes proliferation and their mutagenicity in salmonella typhimurium TA98.
Materials and Methods: In this experimental study, the plants were collected from different areas of Kurdistan. Then, samples were air dried and powdered and methanol material of plants was extracted. The extracts were diluted to give concentrations of 10, 100, 500, 1000, 1500, 2000, 2500 &mug/ml. Finally, the effects of these extracts on human lymphocytes proliferation and their mutagenecity have been investigated by the MTT and Ames test.
Results: The results showed that different organs extract from both tested plants caused a significant increase in lymphocytes proliferation, specially in concentrations of 500 to 2500 &mug/ml. Of studied excrtacts, the highest and lowest effect on lymphocytes proliferation was obtained in presence of flower and seed, respectively. In total, the levels of proliferation resulted of prangos ferulacea as compared with prangos acaulis were higher. Also, the results of study showed no mutagenicity of studied plant exctracts with considered concentrations.
Conclusion: The findings revealed that both species of prangos can increase immune system function and were used as an safe medicinal plant to cure patients with immune deficiencies and microbial infections.
Saeed Taebi, Mokhtar Nosrati,
Volume 19, Issue 11 (2-2017)
Abstract
Background: In the recent years, introducing plant materials with the ability to destroy or control of biofilm formation of Streptococcus mutans is highly regarded.This study was planned to investigate the anti-bacterial activity and biofilm inhibition of Satureja Khuzestanica Jamzad against biofilm and single form of S.mutans.
Materials and Methods: The plant material collected, air dried and powdred. Then thier methanolic extract prepared by maceration method.The concentrated extract then diluted by sterile phosphate buffer.The anti-bacterial and biofilm inhibition activity of the extracts evaluated by dick diffiusion and micro titeration method respectively. Also, bioinformatic study of glucansucrase inhibition by dominant plant compounds of S.Khuzestanica Jamzad investigated by molecular docking method using Auto dock4 software.
Results: The results revalued that all tested extracts especially leaf ectract had significant antibacterial activity against single form of S.mutans.Results also showed that leaf and stem extract had most and least biofilm inhibition with 70 and 13 presend inhibition, respectively. The bioinformatics results also confirmed that studied phytochemicals especially gama-terpinen, carvacrol and beta-bisabolene can effectively inhibit the glucansucrase.
Conclusion: The results of present study showed that S.Khuzestanica Jamzad had significant antibacterial activity against single form of S.mutans and gama-terpinen, carvacrol and beta-bisabolene are most effective compounds in biofilm inhibition.
Mokhtar Nosrati, Zahra Shakeran, Zainab Shakeran,
Volume 20, Issue 5 (8-2017)
Abstract
Abstract
Background: Hepatitis B virus infection (HBV) is a significant global health problem and is a major cause of morbidity and mortality worldwide. Therefore, currently, introducing novel anti Hepatitis B drugs is taken into consideration. This study was planned to in silico screening novel Hepatitis B virus DNA polymerase inhibitors from two medicinal plants Terminalis chebula and Caesalpinia sappan.
Materials and Methods: This is a descriptive-analytic study. In the study, three-dimensional structure of the Hepatitis B virus DNA polymerase was predicted using homology modeling method. A set of phytochemicals from mentioned plants were retrieved from Pubchem database in SDF format. In silico screening was carried out using molecular docking between mentioned phytochemicals and modeled polymerase by iGemdock 2.1 software.
Results: Results of the study confirmed that all evaluated ligands have appropriate interactions to the polymerase with least toxicity and without genotoxicity potential. Results also showed that most interactions occur in reverse transcriptase domain which located in 354-694 area in the amino acid sequence of tested polymerase. Analysis of energy and amino acids involved in ligand-polymerase interaction revealed that Terchebin, Chebulinic Acid and Terflavin A have more effective interaction with the polymerase in compared to other ligands.
Conclusion: Based on the results it can be concluded that evaluated compounds could be good candidates for in vitro and in vivo research in order to develop novel anti- Hepatitis B drugs.
Hesameddin Modir, Aidin Shakeri, Alireza Ghafouri, Gholamhassann Chaichi Nosrati, Shiva Alikhani, Ayda Khammari,
Volume 28, Issue 2 (4-2025)
Abstract
Background: Some intracranial surgeries must be performed while the patient is awake. Awake craniotomy is an intracranial surgical procedure in which the patient is kept in a state of relative consciousness while the surgery is performed. The aim of this study was to present a case of intracranial surgery to remove a tumor in the right frontal cortex by awake craniotomy.
Case: In this report, anesthesia in craniotomy surgery with full wakefulness was investigated in a 22-year-old man. This patient, who was the first to perform a craniotomy with the fully awake method (from the beginning to the end of the operation) at Arak University of Medical Sciences and Valiasr Hospital in Arak, after preparing the patient and interviewing before the operation, Cranial nerve blocks related to the skin and head muscles (scalp block) were performed in the desired locations, and after providing mild sedation, surgical excision was given. The lesion was examined while awake and the surgery was performed while fully awake from the beginning to the end, and the patient's brain tumor was removed safely. A combination of local and regional anesthesia and light intravenous anesthesia was used in the patient. Two types of local anesthetics, lidocaine 1.5% and bupivacaine 2.5%, were used for anesthesia, as well as intravenous sedatives and anesthetics such as propofol, remifentanil, and dexmedetomidine were used to relieve the patients. Throughout the operation, the patient was awake and breathing spontaneously, and the function of the brain nerves during the operation could be evaluated. Finally, the patient's surgery was performed without any complications and in completely favorable conditions, and the patient was transferred to the intensive care unit in a good general condition, awake and fully alert.
Conclusion: Although maintaining analgesia and hemodynamic and ventilation stability in fully awake craniotomy conditions is considered a challenge, but due to the necessity of performing this procedure due to the need to check the function of cranial nerves during the operation and to prevent any neurological and functional damage, using By carefully performing the desired nerve block in the scalp and prescribing a small amount of sedative medicine(propofol,remifentanil,dexmedetomidine), we were able to create a condition where the operation can be successfully performed while fully awake. In this method, the possible complications of surgery are prevented and a satisfactory analgesia is achieved for the patient. In this recent experience, both surgical complications are prevented and the patient and surgeon are satisfied during the operation. Performing this technique was satisfactory for our patient.
