Journal of Arak University of Medical Sciences

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Effect of Eight Weeks of Endurance Training on Brain Lactate Uptake of Diabetic Rats During Hypoglycemia State

Malihe Aveseh, Rohollah Nikooie, Vahid Sheibani ,
Volume 17, Issue 5 (8-2014)

Abstract

Background: Considering to the frequency of occurrence of hypoglycemia in diabetes, alternative substrates for glucose play an important role in maintaining brain metabolism. The aim of the present study was to investigate the long-term effects of endurance training on brain lactate uptake during hypoglycemia in type 1 diabetic rats.

Materials and Methods: In this experimental study, seventy-two male Wistar rats were equally divided into four groups: Control, Trained, Diabetic control and Diabetic trained. Diabetes was induced by intraperitoneally injection of streptozotocin. After 8 weeks of endurance training, Hypoglycemia was induced by an intraperitoneally injection of insulin. One-half hours after hypoglycemia, lactate (4 mmol/kg) was injected intraperitoneally into animals. Animals were killed at intervals of 10, 20, and 30 min after injection, plasma and brain concentration of lactate and glucose were measured.

Results: A significant difference was found for plasma lactate concentration at intervals 20 (p<0.05) and 30 (p<0.05) between healthy groups and also between diabetic groups (p<0.05). Thirty minutes after injection, significant increase in brain lactate concentration was found between control and trained (p<0.05) and control diabetic and diabetic trained (p<0.05). Brain/plasma ratio of lactate had a similar pattern with brain lactate concentration. Increase in brain lactate concentration had no effect on decreased level of brain glucose concentration.

Conclusion: Endurance training can increase the brain lactate uptake of diabetic rats during hypoglycemia.

Exercise-induced Oxidative Shift of Lactate Dehydrogenase Isoforms in Cerebrospinal Fluid of Male Wistar Rats

Nasrin Shikhhossieni, Rohollah Nikooie, Mohsen Aminaie,
Volume 21, Issue 1 (4-2018)

Abstract

Abstract
Background: The aim of the present study was to investigate the effect of endurance training on the content of lactate dehydrogenase isoforms (LDHA and LDHB) and isozymes in cerebrospinal fluid of male wistar rats.
Materials and Methods: Seventeen animals (5 weeks old) were randomly divided into two groups according their body weight: control (C; n = 9) and trained (T; n = 8). The animals from the T group were underwent a training program for 12 weeks (started at 20 m/min for 30 min and finished at 26 m/min for 60 min at the last week). Cerebrospinal fluid (CSF) samples were collected from cisterna Magna 72 hours after the last exercise session. LDH Isoforms and isozymes were measured by ELISA and Electrophoresis techniques, and the comparisons between groups were evaluated by student t-test.
Results: Both LDHA and LDHB isoforms were found in the CSF of the C group; LDH1 and LDH5 had the highest and lowest content, respectively. Following the twelve-week training protocol, LDHB (p<0.01), LDH1 (p<0.01), and LDH2 concentrations (p<0.01) were significantly increased in the T group compared with those from the C group and LDH3 concentrations (p<0.01) and LDHA/LDHB ratio (p<0.01) were significantly decreased. Endurance training had no effect on LDHA and LDH4.
Conclusion: Endurance training is associated with oxidative shift of lactate dehydrogenase isoforms and isozymes in cerebrospinal fluid which could be considered as a prelude to aerobic metabolism of lactate in the brain.

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