Introduction: One of the methods of cancer treatment is use of chemotherapic drugs. Breast cancer is a common desease that chemotherapic drugs have not treated it yet. In this research, Cell proliferation inhibition in MCF-7 cells, a human breast carcinoma cell line , after treatment with Cisplatin, an anticancer drug is studied.
Material and Method: At first, after cell counting with trypan blue stain, equal cells were added to flasks and flasks were divided in to two control and experimental groups. In experimental group, 1 micro mole cisplatin exposure in 1 hour, where control group with normal saline solution. after that, flasks were incubated for 48 hours. After passing this time, attached cells of two control and experimental groups, were stained with trypan blue for cell counting and with toloiden blue for light microscopy individually. Obtained results were divided into two section, cell counting and light microscopy. In cell counting. Obtained results were evaluated with Mann-Withney Statistical test.
Results: results showed that number of attached cells in experimental group was reduced. In light microscopy, morphological features of attached MCF-7 cells in two groups were similar.
Conclusion: Through that, attached MCF-7 cells are proliferating so we can say that without altering in Morphological features, Cisplatin causes inhibition in cell proliferation of MCF-7 cells.
