Showing 5 results for Nejati
Meysam Ganjibakhsh, Masomeh Asadi, Vahid Nejati, Nowroz Delirezh, Farah Farokhi,
Volume 15, Issue 2 (June-July 2012)
Abstract
Background: Since researchers were able to produce dendritic cells (DCs) from peripheral blood monocytes, many scientists have been in search of discovering the best way of producing dendritic cells and optimizing the DCs maturation processes in vitro to treat some diseases. The aim of this study was to investigate the maturation of DCs for tumor immunotherapy. Materials and Methods: In this experimental study, DCs were produced in two stages. In the first stage, monocyte cells were converted to immature DCs by GM-CSF and IL-4. In the second stage, immature DCs were made mature in the presence of human umbilical vein endothelial cells and PHA -activated T lymphocytes conditioned media and maturation factors. Results: The produced DCs with appropriate phenotype, phagocytosis ability, and proliferation of T lymphocytes stimulation traits could secrete high levels of cytokines. Conclusion: Endothelial cells and T lymphocytes conditioned media can produce Th1 and DC1 in vitro. Therefore, DCs produced through this method are suitable for immunotherapy treatment applications and cancer treatment through treatment cells.
Parisa Nejati, Armita Ghahremaninia, Ali Mzaherinezhad,
Volume 18, Issue 5 (8-2015)
Abstract
Background: Subacromial impingement is one of the most common complaints of shoulder. Treatments include avoiding of painful activities, oral anti-pain drugs, physical therapy modalities, corticosteroid injection and exercise therapy. Some studies have shown that platelet- rich plasma(PRP) is effective on tendinitis and tearing of tendons, ligaments and muscles, but evidence that has proved PRP as a conservative treatment in shoulder pathologies is very limited. This study aims to investigate the effect of PRP injection on relieving pain and improving daily function of patients with shoulder impingement syndrome.
Materials and Methods: In this clinical trial study, patients older than 40 with pain more than three months were included. If they had three of four positive diagnostic clinical tests of shoulder impingement that were confirmed by shoulder MRI, could be injected PRP twice. The time between injections was 1 month. Pain was measured by visual analog scale (VAS) and function was measured by two questionnaires named disabilities of the arm, shoulder and hand (DASH) and western Ontario rotator cuff (WORC) index. Range of motion (ROM) of shoulder was measured in five directions by goniometry . All of these parameters were evaluated before intervention and in 1, 3, 6 months later.
Results: with due attention to a six-month folloe-up, PRR injection was effective in pain reduction and improvement of patient's function (p<0.05). Shoulder Rom increased in all directions except external rotation and the power of shoulder muscles was evidently improved statistically in flexion, abduction and internal toration.
Conclusion: PRP injection could effectively reduce pain and improve daily activities in patients with shoulder impingement syndrome.
Zohreh Karimi Taheri, Mohammad Hosein Aarabi, Ali Nazari Alam, Majid Nejati, Mohammad Shayestehpour, Hamid Reza Gilasi, Afshin Salehi, Mohammad Esmaeil Shahaboddin,
Volume 24, Issue 1 (April & May 2021)
Abstract
Background and Aim: Despite the anti-cancer and antimicrobial properties of licorice extract and lavender essential oil, some factors, such as low bioavailability and biodegradable, limit their therapeutic use. Using nanoparticles is a method to overcome these restrictions. This study aimed to investigate the anti-proliferative effects of nanoemulsion containing licorice extract and lavender essential oil on cancer cells; we also evaluated its antimicrobial properties in vitro.
Methods & Materials: In this experimental study, nanoemulsions, containing licorice extract and lavender essential oil were developed by the spontaneous emulsion method. The anti-proliferative effect of nanoemulsion was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetric method on two cell lines HepG2 and SK-MEL-3. To measure the antimicrobial effect of 4 standard strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Minimum Inhibitory Concentration (MIC) method was used.
Ethical Considerations: This study was approved by the Ethics Committee of Kashan University of Medical Sciences (Code: IR.KAUMS.MEDNT.REC.1396.106).
Results: The results of MTT test on HepG2 cells indicated that the concentrations of 630, 1250, and 2500 μg/mL nanoemulsions caused toxicity to the cell and led to the death of >50% of the cells (IC50=401μg/mL; P<0.05). Evaluating SK-MEL3 cells revealed that except for 75 μg of nanoemulsion, other concentrations induced death in >50% of the cells (IC50 = 82 μg/mL; P<0.05). In addition, nanoemulsions, with antimicrobial properties, were studied in 4 strains of bacteria; the highest antimicrobial properties were observed in Staphylococcus epidermidis.
Conclusion: Nanoemulsion containing licorice extract and lavender essential oil presents antimicrobial and antiproliferative effects on the two cell lines studied. The current study results indicated that the nano emulsification of lavender essential oil and licorice extract can enhance their biological impact; thus, they can be used as a drug formulation.
Hamid Reza Joneidi Jafari, Parisa Nejati, Ahmad Nazari, Azar Moezy,
Volume 24, Issue 6 (February & March 2022)
Abstract
Background: Rotator cuff injuries are one of the most common causes of shoulder pain.
There are different therapeutic approaches in this disease. The evidence of the positive effect of exercise therapy in this pathology is inconclusive. This study aimed to investigate the effect of a complete exercise protocol on improvement of the patients with rotator cuff injuries.
Methods: The patients with shoulder pain received the 12-week exercise protocol in 4 phases if they had confirmed rotator cuff injuries in clinical examination and shoulder MRI.
Pain was measured by visual analog scale (VAS) and function was measured by two questionnaires; disabilities of the arm, shoulder and hand (DASH) and western Ontario rotator cuff index (WORC). Range of shoulder joint motion (ROM) was measured in five directions by goniometry. All of the outcomes were evaluated before intervention and in 6, 12, 24 weeks after starting the intervention.
Ethic: This study was registered in Iranian Registry of Clinical Trials (IRCT20100718004409N13). A written consent was taken from All participants before the study.
Results: In this study Twenty-four patients with rotator cuff tendinopathy and tear were analyzed finally in terms of effectiveness of exercise therapy. The average of their age was 51.7 years old. 14 patients (53.8%) with Supraspinatus tendinopathy, 6 patients (23%) with Supraspinatus tear, 3 patients (12.5%) with Supraspinatus plus biceps tendinopathy, and one patient (4.16%) with Subscapularis plus Biceps tendinopathy were assessed. The pain score (Visual Analog Scale) decreased at all fallow-ups specially after 24 weeks (3±0.1) versus before intervention (8±0.1) (P value < 0.001). Shoulder range of motions by goniometer and also the patients function based on WORC and DASH Questionnaires were improved after 24 weeks versus before intervention that was significant statistically and clinically (P value < 0.001).
Conclusion: A 12- week exercise protocol including strengthening, stretching, PNF and dynamic stabilizer exercises was effective on pain reduction and improvement of function and shoulder joint range of motion improvement in the patients with shoulder rotator cuff tendinopathy and partial tear. The improvement of the variables lasted 6 months.
Mir Saeed Attarchi, Fatemeh Nejatifar, Hamid Mohammadi Kojidi, Zahra Atrkar Roushan, Niloofar Faraji, Maryam Joshan, Fatemeh Rahattalab, Roholah Amini,
Volume 25, Issue 2 (June & July 2022)
Abstract
Background and Aim The high use of pesticides has increased the concern about its possible harm to individual and environment health. Chronic exposure to pesticides has serious effects on different body organs even before the onset of clinical symptoms. In this study, we aim to evaluate clinical and biochemical characteristics of male workers in a pesticide production factory in Guilan province, Iran.
Methods & Materials This cross-sectional study was conducted on 99 male workers exposed to pesticide and 107 people without exposure who were employed in a pesticide production factory in Guilan and selected using a convenience sampling method in 2020. Their demographical data as well as clinical characteristics such as respiratory symptoms (cough, shortness of breath, etc.), neurological symptoms (fatigue, tremors, cramps, muscle weakness, etc.), psychological symptoms (sleep disorders, anxiety, etc.) and skin symptoms (redness, itching, etc.) were collected from the occupational health records of the workers. Finally, the biochemical parameters were compared between the two groups. Statistical analysis was performed in SPSS software, version 16.
Ethical Considerations This study was approved by the Research Ethics committee of Guilan University of Medical Science, Rasht, Iran (Code: IR.GUMS.REC.1399.632). Informed consent was obtained from all participants in this study.
Results The frequency of symptoms such as headache, itchy skin, cough, and sleep disorders was higher in the exposed group (P<0.05). Based on the biochemical findings, blood urea level, creatinine level, alkaline phosphatase, and fasting blood sugar level were significantly higher in the exposed group (P<0.05). Thrombocytosis was observed in 9.09% of exposed workers, which was higher in those with more than 11 years of work experience.
Conclusion Chronic exposure to pesticide can cause thrombosis, changes in biochemical parameters, and clinical symptoms. It is recommended that biological monitoring should be conducted in exposed workers at a shorter interval.
