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Background: Chronic myeloid leukemia (CML) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. The research evaluates the cytotoxic effect of hydro-alcoholic extract of M.polegium before flowering aerial organs on K562 cell line as a model of chronic myeloid leukemia.

Materials and Methods: In this experimental trial, Leaves and stems of M. pulegium before flowering collected from Afoos city and extraction using maceration method. K562 cells were cultured and treated with concentrations of extract (12.5-100 &mug/ml) and different times (24,48,72 hour). Cytotoxicity of M. pulegium before flowering extract against K562 leukemia cells was estimated by the MTT test method. The absorbance was measured using an ELISA plate reader at 540 nm. Survey on data accomplished with the use of SPSS15 software and one-way ANOVA test analysis and Tukey test and p<0.001 was considered significant.

Results: Hydro-alcoholic extract of Mentha pulegium before flowering showed the highest cytotoxic effect (IC50=50 &mug/ml) and 72 hour after treatment on K562 cell line .in other words, hydro-alcoholic extract of Mentha pulegium before flowering extracts a dose and time dependent cytotoxic effect on K562 cell line.

Conclusion: Considering the cytotoxic effect of hydro-alcoholic extract of M.polegium before flowering aerial organs on K562 cells, the plant can be considered as a potential candidate for further studies on CML treatment.

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Background: Leukemiais a malignant and progressive disease of the Hematopoietic tissues of the body. The pistacia atlantica tree base, the geographic in large areas of the Mediterranean and the Middle East is growing. K562Cell class is considered as laboratory model of chronic phase of human CML. We compared the growth inhibitory effects SUZIN as a chemical compared with pistacia atlantica as a combined Zn plant antioxidant capacity in reducing cancer has been studied.

Materials and Methods: Pistachio nut was collected from around Kerman, then they were dried in room temperature and extraction was performed for 48 hours by maceration method. K562 cell class was incubated in medium RPMI-1640 fortified with 10%(v/v) FBS and 50% Streptomycin-Penicillin. Cytotoxic effect of hydro-ethanolic extract of pistacia atlantica against cancer cells K562 was evaluated in three interval by MTT method. Light absorbance by Eliza device was measured in wave length 540 nm. Statistical analysis was performed using SPSS15 software and ANOVA test.

Results: Pistacia atlantica in 24h and in concentration 100&mug/ml andSuzin in48h and in 12.5 &mug/ml,72 h and in 50 &mug/ml induced growth inhibition half of the cells were K562. Results obtained from changes in cell morphology influenced by hydro-ethanolic extract of pistacia atlantica and SUZIN suggest abnormal transformation of cells that probably represents apoptosis and necrosis.

Conclusion: Time and concentration against cytotoxic effect of Pistacia atlantica have the combined effect. Whileiron supplementation, alone time is due. Special concentration of pistacia atlantica having high antioxidant capacity with the Suzincan be considered as a potential target for inhibiting K562 cells in treatment of blood cancer.

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Background and Aim: One of the new technologies in this century is nanotechnology. Nanotechnology is a vast and promising research platform that has opened up a wide range of opportunities in various fields including pharmacy, medicine, electronics and agriculture. One of the applied nanoparticles in the field of nanobiotechnology is silver nanoparticles. One of the most important features of these nanoparticles is the creation of programmed cell death (Apoptosis). This property has created its antiseptic properties against bacteria, fungi, viruses and nematodes. Nanoparticles have better performance against microorganisms due to their high surface-to-volume ratio and higher contact surface. Meanwhile, silver nanoparticles have shown unparalleled antimicrobial activity against a wide range of microorganisms and have recently attracted the attention of many researchers.
Methods & Materials: In this study, a review of all databases, including ISI Web of Science, Scopus, ISC, PubMed, Google Scholar Learners, Noor, related articles were examined.
Ethical Considerations Ethical principles have been observed in writing the article.
Results: The antimicrobial effect of silver nanoparticles depends on the concentration, shape and diameter of the nanoparticles as well as the time of effect and the type of microorganism. The molecular mechanism of these nanoparticles has been through oxidative stress. The mechanism of inhibitory action of silver ions on microorganisms is the loss of DNA replication ability, inactivation of the expression of ribosomal subunit proteins and other bacterial cell proteins and enzymes necessary for ATP production. The effect of silver ions is primarily on the function of membrane-bound enzymes such as key enzymes in the respiratory chain. Thus, similar cellular mechanisms can cause cell death effects in prokaryotes, fungi, and eukaryotes.
Conclusion: The results showed that variables such as type of microorganism, contact time, concentration, shape and diameter of silver nanoparticles had a significant effect on inhibiting microbial growth.