Abdolrahim Sadeghi , Hosein Mozdarani ,
Volume 2, Issue 7 (Summer 1999)
Abstract
This research is performed to assess clastogenic capacity of cimetidine(CT) against clastogenic effect induced by benzene in terms of micronuclei. For this purpose micronuclei assay method was used. In each experimental group m 5 Balb /C mices was used. 24 hours after i. p .injections slides was prepared from bone marrow and frequency of micronuclei in polychromatic erythrocytes (PCEs) and normochromatic erythrocytes (NCEs) was obtained. Results indicated that CT at therapeutic dose (20mg/kg. b. w) can be reduce genetical damages (micronuclei) due to benzene (1010mg/Kb. B. w) with a dose- related manner (p<0.01, = -0.82). Therefore , our results indicated that CT has anticlastogenic capacity. According to above results and relatively long term use of CT, we suggest using it in the cases in which someone exposed to clastogenic agents such as benzene, especially when someone also suffer from diseases such as peptic ulcer.
Andia Seyedi Moghaddam, Mahdieh Salimi, Najmeh Ranji, Hossein Mozdarani,
Volume 25, Issue 1 (April & May- 2022)
Abstract
Background and Aim MicroRNAs (miRNAs) are a class of small non-coding RNAs (17-25 nucleotides) that have been studied in many diseases. miRNAs studies in different cancers have shown that miRNAs may be considered oncogene or tumor suppressor. So far, many studies have shown that miR-17-5p and miR-93-5p are important regulatory molecules in some biological processes, such as cell proliferation, associated with cancer formation. This study aimed to investigate and compare the tissue and plasma expression levels of miR-17-5p and miR-93-5p in patients with ductal carcinoma breast cancer with the normal control group.
Methods & Materials The total RNA (including miRNA) was extracted from breast and plasma tissue samples of cancerous and normal samples. The RNA concentration and purity were confirmed using optical absorbance measurements. cDNA was synthesized, and the expression levels of miR-17-5p and miR-93-5p were assessed semi-quantitatively by SYBR Green-based real-time RT-PCR assay in plasma and breast tissues of ductal carcinoma breast cancer compared with the control normal samples with SNORD47 as internal normalizer. Data were statistically evaluated using GraphPad Prism 8.0.2.
Ethical Considerations This study was approved by the Ethics Committee of the institute (IRAN 52d/4922, 6.10.2016). All study individuals signed a consent form to use their clinical samples and personal data under the physician’s supervision.
Results The expression level of miR-17-5p showed significantly higher expression in tissues and plasma of the cancer group compared with the control group (P<0.0001). It was also significantly associated with tumor stage and lymph node, and ER (estrogen receptor) and PR (progesterone receptor) status (P<0.0001). While decreased expression of miR-93-5p in plasma and tumor tissues was shown to be significantly associated with tumor stage and lymph node involvement (P<0.0001).
Conclusion The data revealed that high expression of miR-17-5p and low expression of miR-93-5p in both plasma and breast tumor might be associated with poor prognosis in breast cancer. However, miR-17-5p, due to the greater change in expression and ease of plasma detection, may serve as a possible non-invasive biomarker for breast cancer’s poor prognosis. Further follow-up studies are required to confirm this finding.
