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Background: Hyaluronidase A is an antigenic protein that is secreted by Streptococcus pyogenes. Nowadays, streptococcal infections are diagnosed by tracking down anti-hyaluronidase A antibodies. In this study, the attempt was made to generate recombinant hyaluronidase A in E. coli. Materials and Methods: In this experimental study, through designing specific primers and polymerase chain reaction (PCR), hyaluronidase A gene was amplified and after purification, it was sub-cloned in plasmid expression vector pET32a. Then pET32a-hylA was transferred to E. coli BL21-DE3-plySs. Protein generation induced by IPTG. The recombinant protein was purified by Ni-NTA kit and its concentration was assayed by Bradford method. Western-Blot analysis was run for verifying the recombinant hyaluronidase A. Results: The nucleotide sequencing of the gene amplified by PCR was the same as hyaluronidase A gene from Streptococcus pyogenes. Production of the recombinant hyaluronidase A via induction by pET32a-hylA plasmid was done through IPTG. The expressed protein was purified by affinity chromatography by Ni-NTA resin. The concentration of purified protein was 500µg/ml. analysis using a mouse anti-hyaluronidase A serum was reacted with the generated protein using Western-Blot analysis. Conclusion: Recombinant HylA protein can be generated in E.coli and the resulting protein maintains its antigenic properties desirably.

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Background: Hydatidosis is one of the dengerous zoonotic diseases that cause serious problems for human health, as well as major economic losses for livestock industry. Due to the nature of the parasite life cycle and also the structure of the cyst in human, the control of parasite in community is difficult and its treatment has faced with a major challenge. One of these challenges is inactivating the protoscolices for treatment purposes and preventing secondary cysts. Different chemicals have been used in the treatment of cyst that most of them had serious side effects for the patient. The aim of this study was investigating the scolicidal effects of some herbal extracts in vitro.

Materials and Methods: Liver hydatid cysts were collected from slaughterhouse the cysts fluid containing live protoscolex was aspirated aseptically and stored at 4°C until use. Three concentrations (25, 50 and 100 mg per ml) of each extract (ginger and artemisia) prepared and protoscoleces placed into incubator at 37^oC. The viability of the protoscoleces was determined by eosin staining method at the times 5, 10, 25, 40 and 60 minutes.

Results: The methanolic extract of ginger at the concentration of 100 mg/ml leads to kill all of protoscoleces at 40 minutes. While the artemisia extract in none of   investigated concentrations had not much effect on the protoscoleces.

Conclusion: The study of animal models and complementary tests showed that methanolic extract of ginger can be used as an effective protoscolex for it has high activity.