Laleh Soleimanizadeh, Teymor Agamolaei ,
Volume 5, Issue 2 (Summer 2002)
Abstract
Introduction: The purpose of this research is to examine depression rate in nursing, midwifery and health students and its relation with individual perception about being religious in Hormozgan University of Medical Sciences and Health Services.
Materials and Methods: In this study 299 students (105 male and 194 female) were examined with non-random and easy sampling method. They examine with Beck Depression Test and Alport Religious Guidance.
Results: On the basis of Beck Depression Test 55.5% were without depression, 32.1% mild depression, 11% moderate depression and 1.3% sever and very sever depression. Also with use of two dimension Alport Religious Guidance, 8.7% of subjects had external and 91.3% internal religion. On the basis of other classification of Alport Religious Guidance 70.9% of subjects had high, 21.4% moderate and 7.7% low religion. By the means of x2 Test it shows the rate of depression between girls and boys have not significant difference (p>0.05). In order to access relationship between the rate of depression and religious viewpoint, Pearson Correlation Test was used that results show that it is reverse correlation between them. So that with increased religious viewpoint the rate of depression was decreased (r=-0.6, p<0.05)
Conclusion: In low religion subjects, the rate of depression was more than religion subjects.
Hamid Abtahi, Mohammadjavad Ghannadzadeh, Ali Hatef Salmanian, Ehsan Ghaznavi Rad, Masoud Karimi, Neda Molaei,
Volume 11, Issue 3 (9-2008)
Abstract
Background: In molecular diagnosis of microbial agent, purification of chromosome is very important step. In this study, after cell destruction, DNA replication was done by increasing the denaturation time, without DNA purification.
Methods and Materials: In this experimental study eight different dilution of E.coli (8/100, 4/100, 2/100, 1/100, 1/200, 1/400, 1/800 and 1/1600) solution were madce in D.W, Bacteria were separated by filtration. Polymerase chain reaction method was used to propagate 162 rRNA gene by design primers without DNA Purification. In order to confirme sensitivity of PCR, contamination of 15 different sources of Arak well water wafer was compared by MPN method. For confirmed sensitivity of PCR, 15 sources of water in Arak were examined and compared with MPN method.
Results: Present of bacteria in diution sou tion were confirmed by culture. Polymerale Chain reaction (PCR) data were shown this method is able to recognize bacteria in above dilutions after filtration. This study showed high sensitivity of PCR method in compare to MPN method.
Conclusion: Results were shown without stages of extraction of DNA, PCR were done without losing chromosome. Therefore false negative results were decrease and avoided from difficult phases.
Hadi Ansari Hadipour, Ali Molaei Nezamabadi ,
Volume 17, Issue 3 (6-2014)
Abstract
Background: Our Previous studics hane been shown that iron ions produce oxygen free radical reaction products in the erythrocytes. The present study is evaluating the oxidative effects of aluminum in erythrocytes and plasma proteins and decline of antioxidant power of plasma in operating room personnel.
Materials and Methods: Blood obtained from operating room personnel. Erythrocytes and plasma were incubated aerobically in aluminum containing metal catalyzed oxidation (MCO) system. Total antioxidant capacity of plasma was estimated by FRAP assay (ferric reducing ability of plasma), plasma protein oxidation by carbonyl assay and structural changes in Hb were followed by spectrophotometric analysis and assessment of oxy-Hb, met-Hb and hemichrome.
Results: There was a significant relationship between optical density of Hb in 340 and 420 nm and between oxy-Hb and optical density of Hb in 420 nm(p<0.05) On the other hand, 68 to 56% decrease in total antioxidant power of plasma in control group and operating room personnel, results oxidation in plasma protein and carbonyl concentration was increased three fold.
Conclusion: These findings may be helpful in evaluating the antioxidant status and structural changes in Hb and plasma proteins in people who are exposed to aluminum especially operating room personnel.
