Showing 6 results for Koohi
Abbas Tabatabaii, Gholamreza Mihajeri, Mozafar Hashemi, Mohammad Reza Mohajeri, Sheida Savadkoohi,
Volume 10, Issue 4 (12-2007)
Abstract
Introduction: Due to the absence of frozen section in the majority of hospitals, malignant breast masses are being operated in two admissions first for biopsy and second for mastectomy. With simple and rapid technique of touch imprint (intraoperative cytology) both operations can be done in the same time. In this study the diagnostic accuracy of intraoperative cytology (touch imprint) in malignancy of breast masses is evaluated. Materials and Methods: During 2003 to 2006, reports of touch imprints of 70 patients with breast masses were compared to reports of their permanent section. Based on gathered data, positive and negative predictive value, sensitivity and specificity, and positive and negative likelihood ratio of the test were determined. Results: In 70 patients, 39 cases had malignant and 31 cases had benign masses. In 39 patients with malignant masses, 36 patients had malignant and 3 patients had benign cytologic reports. In 31 patients with benign masses 2 patients had malignant reports in cytology. Therefore sensitivity, specificity and accuracy of touch imprint in diagnosis of malignant breast masses were 92.3%, 93.5% and 92.9% respectively. Conclusion: Because of high specificity of touch imprint, it seems that this test can be done in centers that do not have frozen section for intraoperative diagnosis of malignant breast masses.
Azra Kenarkoohi, Hoorieh Soleimanjahi, Shahab Falahi, Hossein Riahi Madvar, S Zahra Meshkat,
Volume 13, Issue 4 (1-2011)
Abstract
Background: Based on the severity and prognostic condition of respective cancers caused by them, papilloma viruses are classified into high, medium, and low risk groups using E6 and E7 viral proteins. Nowadays, different methods of modeling in clinical medicine are used for diagnosis of diseases and evaluation of their molecular characteristics. Among the new methods of modeling, fuzzy systems are of particular importance in various fields of science. The aim of this study was to use a new intelligent Adaptive Nero Fuzzy Inference System (ANFIS) for predicting human papilloma virus oncogenicity based on a number of biochemical properties of E7 protein. Materials and Methods: In this study, using ANFIS model, a new model was developed for predicting oncogenicity of papilloma virus isolated from patients. The process of training and testing was performed using a set of available published filed data and several statistical and graphical criteria. Accordingly, through provision of needed biochemical and biophysical data on E7 gens from the existing data, this model was developed. The results of this model were, then, validated by the authentic published data. Results: Based on the results, the developed model is capable of predicting papilloma virus oncogenicity efficiently. R2 and RMSE values in training stage were 0.99 and 101.18, respectively. In the testing stage, however, they stood at 0.94 and 173.8, respectively. Conclusion: Based on the findings, the use of ANFIS model significantly improves the accuracy of estimating virus oncogenicity phenomenon. The methodology presented in this study is a new approach in estimating viral oncogenicity and can successfully be combined with other mathematical models for model updating in real conditions.
Azra Kenarkoohi, Mehrdad Ravanshad, Manoochehr Rasouli, Zohreh Sharifi, Shahab Falahi,
Volume 14, Issue 1 (3-2011)
Abstract
Background: TTV is the first human circoviridae that was isolated from Japanese patients with unknown hepatitis in 1997. Since then, several studies have been done on different aspects of TTV pathogenesis. The aim of this study is to determine the prevalence of TTV in patients with chronic hepatitis using two different primer sets. Materials and Methods: In this descriptive study, blood samples from 240 patients with chronic hepatitis C at Professor Alborzi Clinical Microbiology Research Center were assessed in terms of the presence of TTV DNA in plasma through the nested polymerase chain reaction using two primer sets. Results: Of the 240 patients, TTV-DNA was detected in 220 (92%) patients with chronic hepatitis C using 5΄-UTR primer and in 12 (5%) patients using N22 primer. According to the demographic data, there was not a significant difference between male female patients in prevalence of TTV infection. Conclusion: The prevalence of TTV DNA in plasma samples from patients with chronic HCV by using 5΄-UTR primer was high and it was congruent with studies done in other countries however, N22 primer showed a lower prevalence of viral DNA in the samples. Overall, there was not a significant correlation between sex and the presence of viral DNA in patients. Controversial or high prevalence of this virus in HCV infected people necessitate further studies for determining the relationship between HCV and TTV infection.
Shahab Falahi, Mehrdad Ravashad, Azra Kenarkoohi,
Volume 14, Issue 4 (9-2011)
Abstract
Background: Polymerase chain reaction is the most common technique in the field of molecular biology that use for amplification of a specific nucleic acid sequence. Degenerate primers have ability to amplify related but distinct sequences. The aim of current study was to use, two sets of degenerate primers in combination with Hemi Nested PCR for detection V3-Loop sequence of envelope gene from wide spectrum of Human Immunodeficiency Virus (HIV) subtypes.
Material and Methods: In this experimental study we designed and optimized, a degenerate primer pair in combination with Hemi Nested PCR, to detect HIV-1 V3 loop from Envelop gene that has wide variations among genotypes. The developed assay was used to check, 40 HIV infected, 10 negative controls as well as 5 samples from each HCV, HBV, HGV, and TTV were analyzed using developed method.
Results: after optimization, 35 out of 40 positive controls were positive using our test. None of the negative human and viral control samples showed specific band. Also, in positive samples, non-specific bands were not detected.
Conclusion: In this study moreover than standard PCR, we used two degenerate primers that could detect specific region of genome. In fact, first round of PCR product act as a template for second round inner primers and can produce smaller sequence with high sensitivity due to degeneracy. Based on the current investigation, the developed assay had advantages including product confirmation and hence more sensitivity.
Fatemeh Koohi, Mostafa Enayatrad, Hamid Salehiniya,
Volume 18, Issue 3 (6-2015)
Abstract
Background: Cancer in the elderly is rising increasingly. According to the population structure in Iran, it is necessary to conduct a study to examine the epidemiology and trends in cancer incidence in the elderly in Iran. So, the aim of this study was to investigate the epidemiology and trends in cancer incidence in Iranian elderly from 2003 to 2009.
Materials and Methods: This cross-sectional study was conducted by analyzing existing data obtained from the National Cancer Registry System in Iran. In this stucly, all records of elderly cancer cases in Iran during 2003 to 2009 were investigated. The age- specific incidence rates of reported cases were estimated by using direct standardization method and the world standard population. We used Cochrane Armitage Test for linear trend by Winpepi software to study the trends in cancer incidence.
Results: stuolying statistics of Cancer Registry center shows that the incidence of cancer increases with age and most cases of cancer occur commonly in elderly people (aged over 60), so that, the highest number of cancer cases diagnosed in people aged 80-84, but it decreases after the age of 85. The most common cancer among the elders, in this period (2003-2009), was the skin cancer. Also, according to the statistics, cancers are more prevalent among older men than older women.
Conclusion: with due attention to the results, the trend of incidence of cancer and its various types in the elders is increasing with age. The implementation of screening programs and necessary trainings in the field of early detection and the avoidance of exposure to risk factors to reduce the risk of cancer in Iranian elderly can be useful.
Naghmeh Shokoohi Nejad, Mohammad Reza Bayat, Firoozeh Zanganeh Motlagh,
Volume 25, Issue 1 (April & May- 2022)
Abstract
Background and Aim Fibromyalgia syndrome is a chronic disease that causes widespread musculoskeletal pain. It primarily affects women and negatively impacts many aspects of a person’s psychological and social life. This study aimed to compare the effectiveness of cognitive therapy based on mindfulness and compassion therapy on automatic negative thoughts, psychological symptoms, pain intensity, and quality of life in patients with fibromyalgia syndrome.
Methods & Materials This research is a quasi-experimental study with a pre-test-post-test design with two experimental and one control group. The statistical study population consisted of all individuals with fibromyalgia syndrome referred to Mehregan Pain Clinic in Mirdamad (District 3), Seyed Khandan Clinic (District 4), and Taleghani Hospital Pain Clinic (District 1) in Tehran City, Iran. Of them, 30 patients were randomly selected and divided into three groups of experimental 1, experimental 2, and control; each group will include 10 people. The instruments used in this study included the World Health Organization quality of life questionnaire (WHOQOL BREF), pain intensity questionnaire (PIS), psychological symptoms questionnaire (Anxiety, Stress and Depression [DASS]), and automatic negative thoughts (QNS).
Ethical Considerations This study was approved by the Research Ethics Committee of Arak Branch, Islamic Azad University (Code: IR.IAU.ARAK.REC.1400.005).
Results The analysis of covariance showed the effectiveness of both cognitive therapies based on mindfulness and compassion therapy on four variables of pain intensity, psychological symptoms, automatic negative thoughts, and quality of life (P<0.05). Comparing the two intervention methods showed that both therapies had almost the same effect on pain intensity, psychological symptoms, and automatic negative thoughts (P<0.05) but the effectiveness of mindfulness intervention on the quality of life was significantly higher than compassion intervention (P<0.05).
Conclusion The results indicate that cognitive therapy based on mindfulness and compassion therapy are suitable for people with fibromyalgia under appropriate treatment.
