Hadi Ansarihadipour, Hasan Ziafatikafi,
Volume 14, Issue 6 (February-March 2012)
Abstract
Background: Biochemical studies have shown that iron produces reactive oxygen species via Haber-Weiss and Fenton reactions. The goal of this study is to examine the role of iron in oxidation of human hemoglobin and its structural changes in erythrocytes.
Materials and Methods: In this experimental study, blood samples from healthy subjects were incubated aerobically with the iron containing metal catalyzed oxidation (MCO) system in the presence of 0.036, 0.7, 0.14, 0.28, 0.57, 1.14, 2.28, 4.55, 9.09, and 18.18 micromole of iron. Structural changes in Hb were followed by spectrophotometric analysis from 300 to 650 nm. In addition, carbonyl assay was performed for estimation of protein oxidation in globin chains.
Results: Based on the results, oxy-Hb decreased up to 68% in iron-treated erythrocytes. Decrease in the absorbance ratio (A577, A542 wavelength) indicated the conversion of oxy-Hb to met-Hb. Also, met-Hb concentration was 4.7 fold of hemichrome. After 24 hours of incubation, oxyHb concentration decreased up to 50% and metHb concentration increased up to 85%. Moreover, increase in iron concentration resulted in significant carbonyl formation in hemoglobin.
Conclusion: These findings indicate that Hb oxidation instead of its oxygenation leads to anemia and hypoxia. The findings of this study may be directly applicable to oxidation states during hemolytic diseases and iron treatment.
Farzin Bagheri Sheykhangafshe, Abbas Abolghasemi, Seyyed Mousa Kafi Masouleh,
Volume 24, Issue 2 (June & July 2021)
Abstract
Background and Aim: The concept of resilience has been recently extensively considered concerning sports studies; accordingly, in most qualitative studies, it has been used to explore the attitudes of coaches, athletes, and sports psychologists. In this regard, the present study aimed to determine resilience in athletic students based on dark triad personality and psychological wellbeing.
Methods & Materials: This was a descriptive and correlational study. The population of the study consisted of all male students of Guilan University of Physical Education in the first semester of the academic year 2019-2020. A sample of 200 individuals was selected by convenience sampling method; then, the required data were collected using Sheard et al.’s (2009) Resilience Scale for Adults, Jonsson and Webster’s Dark Triad Personality Questionnaire, and Ryff’s 18-item Psychological Wellbeing Scale (1989). The obtained data were analyzed by SPSS using Pearson correlation coefficient and multiple regression analysis.
Ethical Considerations: This study was approved by Research Ethics Committee of Guilan University of Medical Sciences (Code: IR.GUMS.REC.1398.121).
Results: The present research results suggested a positive and significant correlation between the resilience of the examined athletic students and narcissism (r=0.495, P<0.001), self-acceptance (r=0.598, P<0.001), purposefulness in life (r=0.556, P<0.001), personal growth (r=0.648, P<0.001), the mastery of the environment (r=0.645, P<0.001), positive correlation with others (r=0.569, P<0.001), and independence (r=0.381, P<0.001). Moreover, there was a significant negative correlation between antisocial behavior (r=-0.557, P<0.001) and Machiavelli’s aspect (r=-0.604, P<0.001), and the explored students’ resilience. Additionally, multiple regression analysis data revealed that dark triad personality and psychological wellbeing predict 62% of resilience in the study subjects.
Conclusion: The current investigation results signified a significant correlation between dark triad personality and psychological wellbeing, and resilience in athletic students. Accordingly, educators and teachers need to pay more attention to the role of psychological wellbeing and different dimensions of athletes’ personalities for promoting the health and performance of students.
Neda Kafi, Amene Barjaste Yazdi, Rambod Khajei, Mohammadreza Hoseinabadi,
Volume 26, Issue 2 (June & July 2023)
Abstract
Introduction: The purpose of this research was to investigate the effect of a period of resistance training and melatonin consumption on sex hormone levels, pain intensity, and sleep quality in girls with primary dysmenorrhea.
Methods: For this purpose, 60 girls with moderate primary dysmenorrhea disorder (score 4-7) were randomly placed in 4 groups: resistance training + melatonin, resistance training + placebo, melatonin supplement group, and control group. Pain intensity, and sleep quality were assessed by the McGill questionnaire, and the Pittsburgh questionnaire respectively. 10 mg of melatonin was taken daily in two 5 mg capsules and the placebo group received the same amount of carbohydrates in the same capsule. Weight training, three days a week for eight weeks. This circuit exercise training was in 9 stations and with a maximum of 10-12 repetitions at 30-65% of a maximum repetition in each station. Each set and repetition was separated by 2-3 minutes and 90 seconds of rest, respectively. The blood sample was taken in the morning, fasting and 5 cc from the brachial vein from the left hand while sitting in the sitting position, and was taken by special kits. Data were analyzed by ANOVA with repeated measures and Bonferroni post hoc test at level P<0.05. All experimental procedures were approved by the Ethics committee of the Sport Sciences Research Institute of Iran (Code: ID IR.IAU.NEYSHABUR.REC.1401.008), Clinical Trial (Code: ID IRCT20230703058653N1) from the Iran Clinical Registration Center and were conducted under the Declaration of Helsinki.
Results: A significant increase in Estrogen (P<0.001) and progesterone (P<0.001) levels in the exercise + supplement group compared to all groups, and in the exercise + placebo group and the supplement group compared to the placebo group was reported. Also, The decrease in Pain intensity (P<0.001) and sleep quality (A decrease in the sleep score means an increase in the sleep quality in the output of the questionnaire) (P<0.001) in the supplement group compared to all groups and in the exercise + placebo group (P<0.05) and the exercise + supplement group compared to the placebo group showed a significant difference.
Conclusions: It seems that synergy of exercise and melatonin has affected the sex hormones level. Also, the melatonin probably helped to improve the sleep quality and pain intensity of the subjects through the adjustment of sex hormones following dysmenorrhea. Also, exercise probably has an effect on the pain intensity and sleep quality through the release of beta-endorphins and the effects of exercise on the menstrual cycle, although the finding are ambiguous.
