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Introduction: Life expectancy is one of the summary measures of population health, whose values and trend of changes over time show the main outcome of health system performance. Estimation of life expectancy is performed with direct or model-based methods. In this study direct estimation of life expectancy for Iran’s population in year 2003 is performed. Materials and Methods: In an ecologic study, for calculation of abridged period life table for 23 out of 28 provinces of Iran in year 2003, numbers of registered deaths for age and sex specific groups in these 23 provinces by death registration system of Ministry of Health (MOH) were used. Undernumeration of over-four year's deaths was estimated and corrected with Brass Growth-Balance method. For mortality rates of under-one and 1-4 years, similar rates from Demographic and Health Survey (DHS) 2000 with live births denominators were substituted. For total number of population of 23 provinces, information from MOH was used. Distribution of population by age and sex was taken similar to that from DHS 2003 results, and also taken as result of graduation of whole country population from 1375 census. Population of these 23 provinces was equal to 73% of total population of country in year 2003. Life expectancy was estimated by age and sex with the obtained population and death numbers. It was assumed that distributions of population and death in 23 provinces were similar to those for the whole 28 provinces of Iran in year 2003 and hence, the estimated life expectancy for 23 provinces is equal to that for all 28 provinces in 2003. Sensitivity of the results to assumptions was analyzed. Confidence intervals were calculated with Monte Carlo method. Results: Life expectancy at birth was estimated as 71.56 years for total population (95% CI: 71.52 – 71.62), 70.09 years for males (70.02 – 70.16), and 73.17 (73.10 – 73.24) for females of Iran in year 2003. Sensitivity of results to assumptions was less than 0.5%. Conclusion: Values of life expectancy estimates for year 2003 with direct method are higher than those based on statistical modeling approaches performed by Statistical Center of Iran and by different United Nations agencies, due to difference in estimation methods for age and sex specific mortality rates.

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Background: Urinary incontinence is a very common problem affecting the quality of life of many people, especially women. Hence, this study was done to investigate the results obtained from intravaginal slingplasty (IVS) method in treating stress urinary incontinence (SUI) in patients.

Materials and Methods: This retrospective study was carried out as census on female patients suffering from SUI who had been treated using IVS method in Najmieh Subespeciality Hospital, Tehran, during 2004-2009 years. Data were extracted through interviews and from patients’ medical records. Descriptive statistics (mean and percent) and inferential statistics (independent t-test and its exact version, Bootstrap, Chi-square test, and Fisher’s exat test) were utilized and data were analyzed using SPSS software.

Results: A total of 357 women with mean age of 45.18±8.78 years participated in this study. Recovery rate of emergency and stress-related SUI by IVS method were 80.1% and 98%, respectively. In addition, urgency SUI had statistically significant correlations with diabetes, blood pressure, age, total number of deliveries, and normal vaginal delivery (NVD). Also, stress-related SUI had significant correlations with post-void residue (PVR), total number of delivery, NVD, and cesarean section.

Conclusion: Generally, it can be concluded that treatment response of IVS in this study is comparable and even better than other existing and customary modes of treatment

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Background: Solid waste is one of the environmental pollution. Therefore the aim of this study was to survey of knowledge, attitude and practice of student and teachers about weast management for developing methods of educational in Arak city.

Materials and Methods: This is a cross sectional and analytical study that was carried out on 1739 student and 149 teacher in school of Arak city in 2012. sampling with cluster and data collection with questionnaire including knowledge, attitude, practice and appropriate resource of education for waste management. Finally data analyzed with statistical tests.

Results: The mean knowledge and attitude student was 4.1±1.9 (out of 8) and was 20.63±5.9 (out of 35) respectively. Based on most educational methods for weast management viewpoint of student book 441 student (29% ) but impact of internet very low interest 487 (32%) of student. Moreover the most educational methods based on level of education significant difference was observed (p=0.001).

Conclusion: Due to a level of knowledge and attitude of student and accsses to educational resourse and huge of weaste in school educational program planned via book, teacher and peer education for student recommended.

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Background: Lamiaceae family has about 200 genuses and 4000 species. The majority of species of the family have essence that was used for nutritional, cosmetic and pharmaceutical industry. The aim of this study was to investigate the chemical composition of essential oil from aerial parts of Bollota platyloma  and antibacterial activity of it.

Materials and Methods: In this experimental study, study Ballota platyloma, belonging to lamiaceae family and entomic of iran, was collected from Versk region in Mazandaran provinc. Essential oil from aerial parts was obtained by hydro distillation method by using Clevenger apparatus. GC/MS analysis of essential oil was carried out to identify major volatile constituents. Extraction was prepared by maceration method. Antibacterial activity of methanolic extracts against Pseudomonas aeruginosa, Bacillus subtilis, Stapylococcus aureus, Escherichia coli was investigated by disc diffusion method and minimal inhibitory concentration(MIC) method.

Results: Results showed that GC/MS analysis of the essential oil confirms the presence of 24 constituents in Ballota platyloma. The main constituents were Hexadecanoic Acid (40.03%), Germacrene D (26.6%) and Beta Caryophylene (4.76%). The results indicated that methanolic extract of Ballota platyloma possessed antibacterial activity. Among the aforementioned bacteria, the highest antibacterial activity was seen against S. aureus and the lowest activity against P. aeruginosa.

Conclusion: The findings of the present study showed that the major component of oil essential was germacrene-D and methanolic extract from the aerial parts of Ballota platyloma Rech. f. possess anti-bacterial effects. Thus, in order to find the underlying mechanism of this activity, further research should be carried out.

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Background: Hypervariability of hepatitis C virus (HCV) proteins is an important obstacle to design an efficient vaccine for the infection. To construct a protective vaccine against HCV, a DNA vaccine containing conserved epitopes of the virus was designed. To enhance the induced immune responses, adjuvant activity of N-terminal domain of gp96 (NT(gp96)) was used.

Materials and Methods: A multi-epitope (PT) DNA vaccine encoding four HCV immunodominant cytotoxic T lymphocyte epitopes (HLA-A2 and H2-D^d) from Core, E2, NS3 and NS5B antigens in addition to a T-helper CD4+ epitope from NS3 protein and a B-cell epitope from E2 protein was designed and constructed. Then, NT(gp96) was fused to the PT DNA (PT-NT(gp96)). The stimulated cellular and humoral immune responses of PT and PT-NT(gp96) were evaluated in mice model.

Results: According to multicolor flow cytometry assay, the frequency of CD8+ T-cells producing IFNγ and TNFα in the splenocytes of immunized mice with PT-NT(gp96) (6.8%, 4%) was significantly higher than those of immunized with PT (0.9% , 0.8%), respectively. The same results have obtained in hepatic lymphocytes of the vaccinated mice. The level of IgG, IgG1 and IgG2a in the mice vaccinated with PT-NT (gp96) was significantly higher than the value obtained from the mice immunized with PT.

Conclusion: The results showed that PT DNA vaccine induces immune responses in mice model. Fusion of NT (gp96) to PT DNA vaccine causes to enhance cellular and humoral immune responses against HCV compared to sole PT vaccine.

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Background: The failure time of permanent tooth is of the form of interval censored since the exact time of tooth decay is not available and it is only known that tooth decay occurs between two consecutive visits. There are a few techniques available in the literature for the problem of goodness-of-fit for interval censored data. In this paper, we propose a new goodness-of-fit testing procedure for interval censored data and employ this for the failure time of the first permanent molar tooth (sixth tooth) data.

Materials and Methods: Two methods of goodness-of-fit for interval censored data that are based on randomly generated data from each interval and averaging over the test statistics or over the p-values are employed for the failure time of the first permanent molar tooth data.

Results:  The mean of the failure time of the first permanent molar tooth is found to be at 95 months. The p-values of the two goodness-of-fit testing procedures for the Weibull, log-normal and gamma models are calculated.

Conclusion: By comparing the p-values, the log-normal model is considered as the best model to describe the failure time of the first permanent molar tooth data.

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Background & Aim: This study aimed at comparing efficacy of cognitive behavioral therapy, Zolpidem 10 mg, and waiting list group on illness perception and sleep efficiency in individuals with chronic insomnia disorder. 
Materials & Methods: Participants included 74 (female = 43) individuals with chronic insomnia disorder who were recruited from 2018 December to 2020 February by purposive sampling (inclusive & exclusive criteria). Then, patients randomly allocated to one of the three conditions, including cognitive behavioral therapy (N=25), pharmacotherapy (Zolpidem 10 mg.; N=29), and the waiting list (N=20). All patients were assessed three times at pretreatment, post-treatment, and 3-month follow-up by the Persian version of Brief Illness Perception Questionnaire and Sleep Efficiency Index. The data were analyzed by mixed analysis of variance with Bonferroni post-hoc test and repeated measure analysis of variance. 
Ethical Considerations: All stages of the research have been carried after taking supervising and approving of Kharazmi University's ethics in research committee. 
Findings: The patients who received cognitive behavioral therapy compared to patients in waiting list group, obtained significantly lower scores in illness perception and sleep efficiency during post-treatment and 3-month follow-up. Efficacy of pharmacotherapy only observed during post-treatment but there were no significant differences between pharmacotherapy and waiting list patients during 3-month follow-up.
Conclusion: Cognitive behavioral therapy for insomnia reduced significantly illness perceptions and sleep efficiency during 3 months. All the treatment gains remain stable even 3 months later treatment ends. In addition, not receiving any treatment in waiting list and gradually discontinued the treatment in pharmacotherapy group leads to decreasing of sleep efficiency and increasing of illness perception.