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Background: In order to selected indigenous potential probiotic bacteria, we surveyed antagonistic activities of 22 strains of acid and bile tolerant Lactobacillus, isolated from traditional dairy products by biochemical and molecular methods. Methods and Materials: In a fundamental practical study assessment of antimicrobial activity of this strain with neutrallized and Dual layer two methods against bacterial pathogene such as E-coli, L.monocytogenes, S.auteus and Y.entercolitica was done. These strain were identified with two methods for determining of biochemical and sequence of 16Sr DNA. Results: Dual layer method based on the growth of zone diameter were estabilished in three groups of strains inhibitors, semi inhibitors and non inhibitors. Neutralize method around well acidic extract containing strains C5i4, Y144, K213, C4i2, C612 and neutral extract C5i4 zone blight strains was observed. Based on the results, sequence area 16Sr DNA of four strains inclulde C4i2, C1d2, Y2c4, D3b1 indicator bacteria that revealed the highest percentage of inhibitor effect of bacterial indicators, were duplicate and sequency. So four strains L.Bacilus Pentosus, L.Bacillus Bervis and L. Bacillus Paraplantarum, were indentifed respectivey. Conclusion: It seems that indigenous lactobacillus from Iranian dairy products have potential as probiotics. So use of them as bio preservative prevent food bacterial contamination.

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Background: Leukemia is a malignant and progressive disease. Over-expression of inhibitors of apoptosis proteins (IAPs), such as survivin and its anti-apoptotic variants, including sur-ΔEx3, is the main cause of resistance to apoptotic effects of chemotherapy drugs. In the present study, the effects of CBX on apoptosis and expression level of survivin and sur-ΔEx3 and K562 cells (experimental model of chronic myeloid leukemia) were investigated. Materials and Methods: In this experimental study, human K562 cells were cultured and exposed to CBX. Trypan blue exclusion test was used to evaluate growth inhibitory and viability effects of the drug. Fluorescent microscopy (acridine orange/ ethidium bromide double staining) and DNA electrophoresis were applied to the study of apoptosis. The expression level of survivin and sur-ΔEx3 was studied by semiquantative RT- PCR. Results: The results showed that after the 48 h treatment of K562 cells with 150 µM CBX, significant growth inhibitory and apoptotic effects (up to 50%) were induced. In addition, after 2-4 h of treatment with CBX (150 µM), down-regulation of survivin and sur-∆Ex3 were observed. However, the expression level of survivin and sur-ΔEx3 increased to the level of control cells with longer treatment times (6-12 h). Conclusion: Noticing the apoptotic and down-regulatory effects of CBX on survivin and sur-∆Ex3 expression, this drug can be used as a potential candidate for further studies on CML treatment, especially for inhibition of drug resistance in leukemia cells.

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Background: To date, several drugs have been proposed for the treatment of acute promyelocytic leukemia (APL) however, none of them has resulted in complete remission. Therefore, many efforts are in progress to find new drugs with the capability of inducing apoptosis. Recently, anti-carcinogenic effects have been reported for a drug named carbenoxolone (CBX) on several cell lines. In the present study, the effects of CBX on NB4 cell line, as an experimental model of APL, were examined. Materials and Methods: In this trial, NB4 cell line was cultured and treated with different concentrations of CBX (50-250µM) in various time intervals (12-48 hours). Trypan blue exclusion test was used to evaluate growth inhibitory and viability effects of the drug on NB4 cell line. Fluorescent microscopy (acridine orange/ethidium bromide double-staining) and agarose gel electrophoresis DNA were used to study apoptosis. Results: CBX induced growth inhibition of NB4 cells so that growth inhibition rates of NB4 cells, after the 48 hour of treatment with 50, 100, 150, 200, and 250 µM CBX were 32.65, 47.52, 60.73, 68.91, and 74.33%, respectively. Furthermore, the results of DNA fragmentation and fluorescent microscopy assays indicated that apoptosis is a major mode of cell death after treatment of NB4 cells with above concentrations of CBX. Conclusion: Noticing the growth inhibitory and apoptotic effects of CBX on human promyelocytic leukemia NB4 cells, it can be considered as a potential candidate for further studies on APL treatment.