Ahmadi, Moosavi, Hosseinpour Feizi,
Volume 13, Issue 3 (9-2010)
Abstract
Background: Recently, reports have been made of the effects of boric acid (BA) on cancer prevention and inhibition of cancer cell proliferation. This study was designed to investigate the effects of this compound on K562 cell line as a model of chronic myeloid leukemia (CML). Materials and Methods: In this experimental trial, K562 cell line was cultured in the presence of 0.75 to 12 mmol concentrations of boric acid for 24, 48, 72, and 96 hour intervals. Anti-proliferative and cytotoxic effects of BA were measured by trypan blue exclusion test and MTT assay, respectively. Flow-cytometery was utilized for evaluating the effects of BA on cell cycle. Wright-giemsa staining was used for determining the effects of BA, and latex phagocytic assay was used for evaluating the phagocytic potential of the differentiated cells. Results: BA induced growth inhibition of K562 cells in a dose and time dependent manner after 96 hours of treatment with 12 mmol BA, cell proliferation of K562 cells was inhibited to about 83% (p<0.001). In addition, BA induced G1 cell cycle arrest in a way that for instance, after 6 days of treatment with 9 mmol BA, 98% of cell populations were at G1 level. Wright-giemsa staining and latex phagocytic assay results confirmed that K562 cells differentiated toward monocyte-macrophage lineage. Conclusion: Noticing the anti-proliferative and differentiating effects of BA, and no evidence of its adverse effects, this compound can be used as alone or in combination with other drugs in CML differentiation therapy.
Dr Zahra Miraki, Dr Masomeh Rostamzadeh, Mr Aras Feizi, Dr Azad Shokri, Dr Mohammad Aziz Rasouli,
Volume 26, Issue 5 (December & January 2023)
Abstract
Introduction: The dentist's level of knowledge and abilities is one of the determining factors in the correct selection and application of dental cements. This study aimed to investigate the level of knowledge, attitude, and practice of general dentists and specialists about the application of different dental cements.
Methods: This descriptive-analytical study was cross-sectional one which conducted on 150 specialist and general dentists in governmental and private centers in Kurdistan province (2019) who performed fixed prosthetic treatments, was done through census and available sampling. The data collection tool was a questionnaire. To analyze data, T-test and ANOVA tests were used in SPSS-22 software. A significance level was considered less than 0.05. Ethical considerations: This study was conducted after the approval of the protocol in the research ethics committee and receiving the ethical code number IR.MUK.REC.1398.302 and according to the Declaration of Helsinki.
Results: The overall average score of dentists' knowledge was 6.5, which was close to and higher than the average scores and had a significant relationship with the grade and place of employment (P<0.05). The overall average score of attitude was 3.4, which was almost equal to the average scores. There was a significant relationship between grade and gender with attitude score (P<0.05). The overall average score of the dentists' practice was 5, which was higher than the average scores, and the practice score had a significant relationship with gender, place of employment, and grade (P<0.05).
Conclusions: The average score of knowledge, attitude, and practice of dentists was average, and in general, the scores of specialists were higher than general dentists. Women had a higher attitude and practice score. People working in the private sector had a higher knowledge score. To increase the knowledge, attitude, and practice of general dentists, there is a need to hold training courses.
