Background: Saccule is part of the vestibular organ that is responsible for balancing the linear acceleration saccular can be evaluated performance by cervical vestibular evoked myogenic potentials (cVEMPs). This study aimed to determine the relationship between time delay and amplitude of the waves of cVEMPs with auditory training in deaf children of primary school age group of Hamadan in autumn 2014.

Materials and Methods: In this comparative cross-sectional study, the case group were children with profound hearing loss received six months of regular auditory training (n=20). The control group of children with profound hearing loss had no history of auditory training (n=20). Both of groups were evaluated by cVEMPs test.

Results:  T-test between the two groups showed no significant difference in the time delays of waves p13 and n23 (p = 0.15, f = 18.58 and p = 0.20, f = 23.32, respectively). However, the difference of the peak-to-peak amplitude of waves p13-n23 was significant (p = 0.00, f = 10.13).

Conclusion: Voice vocalization and more experience of hearing can strengthen the saccular performance.

Background: Circulating microRNAs are promising biomarkers in diagnosis and assessment of cancerous patients. Quantitative Real-time PCR assay is a sensitive test for evaluating the levels of miRNAs expression. Nevertheless, there is no concurrence on selecting appropriate reference genes for qPCR analysis of miRNAs in circulation. Therefore, the current study aimed to select a suitable reference gene for normalizing the RT-qPCR assay results in plasma samples of patients with gastric cancer.

Materials and Methods: Based on previously published studies, three molecules SNORD47, U6 RNA, and miR-103 were selected as the candidate reference genes. After RNA extraction from plasma samples of 40 patients with gastric cancer and 40 healthy individuals, expression levels of these molecules were evaluated using Real-time PCR method.

Results: The results showed that the developed assays are able to diagnose their specified targets by a suitable linear range. By comparing patients and control groups, although the expression levels of miR-103 molecule were not equal between the two groups (p= 0.017), SNORD47 and U6 RNAs had similar expression levels. However, the variations of SNORD47 expression were lower that U6 RNA.

Conclusion: Based on the results of the current study, the SNORD47 molecule has a stable expression levels in plasma samples of patients with gastric cancer and normal individuals and can be used as an appropriate reference gene for normalizing the quantitative data of qPCR assay.