Showing 3 results for Dalimi
Reza Ghasemi-Khah , Abdolhossein Dalimi-Asl , Bijhan Hashemi-Malayeri ,
Volume 6, Issue 2 (Summer 2003)
Abstract
Introduction: Considering the importance of hydatid cyst and its pathophysiological effects on the human body, we attempt to propose a method for killing of hydatid cyst protoscoleces in vitro condition using low voltage direct electrical current.
Materials and Methods: After collecting hydatid cyst from infected organs of slaughtered animals, their protoscoleces were cultured in four separated media of hydatid consisting of uid, RPMI, normal saline and Tris buffer. The protoscoleces with the same media were transferred to an electrolysis device, and then various sets of electric current density were applied. For measuring the survival rate of protoscolcces, the same cells movement as well as Eosin staining were used as standard techniques.
Results: Our findings indicated that the survival rate of protoscoleces in hydatid fluid was dependent on the electric current density and the time of the applied current. In this regard, the highest survival rate of 86.3% was observed when electric current density of 42.96 mA/cm2 was applied for one minute; while the survival rate of 0% was observed when an electric current density of 63.5 mA/cm2 was applied for one minute. In the RPMI, normal saline and Tris buffer media, similar results were obtained.
Conclusion: Low voltage of direct current can be used in destroying of protoscoleces during surgery on different body organs without any injury to host tissue and any relapse of the infection.
Mehdi Mosayebi, Ehsan Ghaznavi, Abdolhossein Dalimi, Mohamad Moazeni, Ghasem Mosayebi, Mahmoudreza Khazaii,
Volume 9, Issue 3 (9-2006)
Abstract
Introduction: There is difference between susceptibility or resistance to infectious diseases such as cystic and alveolar Eclinococcosis in human and animals, that is due to the difference between individual host factors and immunologic responses. This study is done to investigate the resistance and susceptibility markers (HLA) in Hydatid patients and healthy persons. Materials and Methods: This analythical (case-control) study is carried out on 60 patients with confirmed cystic echinococcosis and 30 healthy individuals living in Arak. Blood samples were gathered and tested by microlymphocytotoxicity method. At first diagnostic kits with specific antiserusms for each antigen (28 antigens) were provided and then lymphocytes were separated. After dye and stabling with formalin and based on cells morphology, results were seen by invert microscope. Data was analyzed using Odds Ratio, Relative Risk, Preventive Fraction, Aetiologic Fraction and Chi square test. Results: Results showed that HLA-A1 was significantly higher in patients (p<0.05), and people having this antigen are more susceptible for the infection. In spite, HLA-A10 was higher is healthy individuals (p<0.05) and have a preventive effect in disease involvement. Other investigated antigens had no signigicant difference in the two groups. For more accurate results molecular investigation is needed. Conclusion: In individuals having HLA-A1 there is more chance for cyst growth in confronting hydatosis and this individuals are more susceptible to the disease. But in individuals having HLA- A10 there is less chance for cyst growth in confronting hydatosis and this antigen have a preventive effect against hydatid cyst.
Tooran Nayeri Chegeni, Fatemeh Ghaffarifar, Fariba Khoshzaban, Abdolhosein Dalimi Asl,
Volume 20, Issue 12 (3-2018)
Abstract
Abstract
Background: Acanthamoeba is an opportunistic protozoan pathogen that is known to infect the cornea to produce eye keratitis and the central nervous system to produce lethal granulomatous encephalitis. The overall aim of the present study was to determine the anti-amoebic potential of natural compound Peganum harmala against the trophozoites and cysts of Acanthamoeba in vitro.
Materials and Methods: In this experimental study, a clinical isolate of Acanthamoeba was cultured and genotyped. The ethanolic extract of Peganum harmala was prepared. The trophozoites and cysts were collected by washing in page's saline. Various concentrations (1.25, 2.5, 5, and 10 mg/ml) of the ethanolic extract and polyhexanide 0.02% drop as positive control were tested at three different times (24, 48 and 72 h) on trophozoites and cysts of Acanthamoeba in vitro. The viability of trophozoites or cysts was tested by eozin method, MTT, and flowcytometry analysis.
Results: The results revealed that alcoholic extract had remarkable inhibitory effect on the proliferation of Acanthamoeba cysts as compared to non-treated control, and the inhibition was time and dose dependent. In the presence of 10 mg/ml ethanolic extract in medium culture after 72 h, no viable trophozoites were determined and 21.10% cysts of Acanthamoeba were viable. Percentage of trophozoites and cysts viability after adding polyhexanide 0.02% drop in medium culture after 72 hours was 0% and 23.71%, respectively.
Conclusion: Ethanolic extracts of Peganum harmala could be considered a new natural compound against the Acanthamoeba trophozoites and cysts. Further works are required to evaluate the exact effect of this extract on Acanthamoeba agents in animal models.
