Maryam Azizpour, Davood Hosseini, Hossein Basiri, Neda Akbari, Mitra Nezamabadi, Saber Eskandari, Mohsen Sarikhani,
Volume 16, Issue 3 (6-2013)
Abstract
Background: Brucellosis is a debilitative disease that imposes heavy costs on the economy and society. Therefore, using the most accurate and efficient method to diagnose this disease is essential. In Iran, Brucella melitensis is the common causative agent for brucellosis and BP26 protein of this bacterium has a good level of antigenicity. Thus, the aim of this study is to produce Brucella melitensis recombinant BP26 protein with a PET28a expression vector.
Materials and Methods: In this applied-fundamental study, genomic DNA was isolated from bacterial culture through proteinase K (pK) and phenol/chlorophorm protocol. Then, two pairs of primers were designed based on the known sequence in the gene bank for amplification of Brucella melitensis bp26 gene and PCR reaction was set up and optimized. The PCR product was cloned first into PTZ57R/T vector and accessed on the PET28a vector and sequenced. The recombinant vector was transformed and expressed into E. coli BL21 (DE3). Then, the recombinant protein was purified with Ni-NTA column of chromatography against His tag.
Results: The size of PCR product was in accordance with the part of bp26 gene size in the gene bank. The bp26 gene without adding IPTG had little expression and 3 hours after adding IPTG with a 1 Mm concentration to culture media, extreme expression was observed.
Conclusion: The production of recombinant BP26 protein from isolated Brucella melitensis native to Markazi province was done. Noticing the importance of BP26 protein and its significance for future studies on providing brucellosis diagnosis kits, its production was made possible.
Sayed Hossein Mousavi, Ali Jafari, Hossein Basirian,
Volume 27, Issue 5 (12-2024)
Abstract
Introduction: The purpose of the "Health Communication" discussion plan, the most serious sparks of which were struck in the minds with the emergence of the COVID-19 pandemic in 2019, revealed the importance of paying attention to improving health communication more than ever. For this purpose, in the current study, the formulation of a conceptual model of health communication capacities in pandemic conditions was examined from the perspective of health system experts.
Methods: This research was conducted using a qualitative approach and the foundation's data theory method. Sampling was done with a purposeful snowball method, which included 20 communication and media professors and health professionals. Data collection was done through semi-structured interviews and document reviews based on the systematic approach of Strauss and Corbin (open, central, and selective coding), which includes causal conditions, central idea, context, mediating conditions (intervenor), strategies, and consequences of analysis. Validation was done through the review of participants and experts in the health system.
Results: The findings of the research in the grounded theory section show that the dimensions of the paradigm model include the central phenomenon: health communication (effective and informative communication), causal conditions: health communication harms (infodemic), the context of harms (widespread and popular media, lack of authority of the health unit, mass information, politicization), strategies (holding officials accountable, reliable sources and producing practical content, anxiety management, avoiding exaggeration of people's participation, teamwork and combination of experts, using the experiences of other countries) and consequences including (attracting trust and forming beneficial relationships) took place.
Conclusions: According to the dimensions achieved in presenting the model for improving health communication capacities in pandemic conditions in the country, it can be said that on the one hand, "transparency and immediacy of information" and the other hand, "empathy and trust between people and officials" in such More than one situation should be considered.
