Showing 5 results for Baazm
Maryam Baazm, Mohammad Reza Darabi, Saeed Babaie, Reza Talebi,
Volume 16, Issue 10 (1-2014)
Abstract
Background: One of the side effects of chemotherapy agents is gonadal suppression leading to infertility. The present study examined the feasibility of using a gonadotropin releasing hormone agonist (GnRH-A) in the prevention of busulfan induced azoospermia in prepubertal mice.
Materials and Methods: In this case and control research, Leuprolide (3.8 mg/kg) was given to prepubertal male mice 2 weeks after or prior to busulfan (40 mg/kg) administration. The animals received one or two doses of leuprolide after busulfan administration with 4 weeks apart. Six weeks after the last leuprolide treatment, the sperm parameters were analyzed icluded sperm count and motility according to the World Health Organization criteria.
Results: At the time of scarifies, testicular weight were increased in animals receiving leuprolide. In animals receiving leuprolide pretreatment, the percentage of progressive and immotile sperm was significantly (p&le0.05) increased and decreased respectively. But the percentage of non progressive sperm did not change. The sperm motility in busulfan treated animals with post treatment didn't change. The sperm count also increased in pretreatment animals but it was not significant (p&ge0.05).
Conclusion: The present study demonstrates that leuprolide pretreatment resulted in improvement in sperm motility in prepubertal male mice.
Fatemeh Samimi, Maryam Baazm, Ebrahim Eftekhar, Farideh Jalali Mashayekh,
Volume 22, Issue 4 (9-2019)
Abstract
Background and Aim Oxidative stress is the main factor in the development and progression of diabetes and its related complications. There is growing evidence that antioxidants supplementation can improve oxidative stress induced in diabetes. The present investigation was conducted to study the effects of Coenzyme Q10 (CoQ10) on the Oxidative Stress Index (OSI) in diabetic rats.
Methods and Materials A total of 30 male rats were divided into five groups: saline, sesame oil (as a vehicle), CoQ10-treated (10 mg/kg/day), diabetic (induced with streptozotocin: 55 mg/kg), and CoQ10-treated diabetic (10 mg/kg/d). Then, we measured the Malondialdehyde (MDA), Total Oxidant Status (TOS), and Total Antioxidant Capacity (TAC) levels in the rats’ liver homogenate. Additionally, the OSI was calculated.
Ethical Considerations The Research Ethics Committee of Arak University of Medical Sciences approved this study (Code: IR.ARAKMU.REC.1397.119).
Results results showed a significant decrease in the level of liver MDA (P=0.022), TOS (P=0.03), and OSI (P=0.028) in the CoQ10-treated diabetic group compared to the diabetic rats. No significant change was observed in the total thiol group (P=0.25) and TAC (P=0.77) level in diabetic rats’ livers treated with CoQ10 compared to diabetic rats.
Mohammad Reza Soleyman, Mostafa Khalili, Alireza Soleyman Meiguni, Maryam Baazm,
Volume 22, Issue 5 (11-2019)
Abstract
Background and Aim recombinant DNA technique is a powerful and appropriate method for the production of protein biopolymers with specificity in amino acid sequence and spatial chemistry. Elastin-Like Polypeptide (ELP) is a biocompatible, biodegradable and non-immunological biopolymer used in various biotechnology studies. The ELP tag is a cheap, fast and non-chromatographic technique for purifying target proteins. In this study, pET expression vector was designed for the combination of ELP gene sequences and target recombinant protein in order to produce recombinant fusion protein with the ELP tag.
Methods & Materials MOD gene was transformed to E. coli-BL21 (DE3) cells after designing and synthesis among the XbaI and XhoI restriction sites in the pET-32a (+) vector of the clone. Then, colonies were isolated based on plasmid size and examined by cutting using restriction enzymes. The final recombinant colonies was verified using polymerase chain reaction method and DNA sequencing.
Ethical Considerations The Research Ethics Committee of Arak University of Medical Sciences approved all ethical considerations ofworking on laboratory animals (Code: 92-146-11).
Results Replacing the MOD sequence in the pET-32a vector (+) eliminated the components expressing the fusion tags (Thioredoxin, Histidine, and S-tag), the identification site of protease enzyme (tobacco etch virus), and multiple cloning site. In addition, it added specific restriction enzyme identification sequences of ELP gene and target gene. As a result, in the optimized pET-MODvector, 466 nucleotides reduced in size and the secondary structure was improved.
Conclusion Considering the improvement of spatial structure and reduction of pET-MOD vector size, as well as the possibility of the fusion of recombinant protein with the ELP tag, it is possible to use this vector for ELPyation of the target protein.
Atefeh Khaki, Maryam Baazm, Mohamad Bayat,
Volume 27, Issue 5 (12-2024)
Abstract
Introduction: Glyphosate is the most popular broad-spectrum herbicide globally due to the growing demand for glyphosate-resistant crops. Glyphosate exhibits harmful properties, including cytotoxicity, neurotoxicity, and reproductive toxicity. This study aimed to investigate the detrimental effects of glyphosate on ovarian histopathology in mice and the in vitro maturation (IVM) of oocytes following superovulation.
Methods: In this study, thirty-two female NMRI mice were randomly divided into the following groups: control, glyphosate, superovulation, and superovulation-glyphosate. Animals received glyphosate (0.5%) continuously through drinking water for three weeks. HMG and HCG were used to induce superovulation. Oocytes were collected from the ampulla, and the quantity and quality of oocytes were analyzed. Then, in vitro maturation (IVM) of oocytes was performed. At the end of the study, ovarian histopathology was analyzed.
Results: Compared to the control group, the glyphosate-treated group exhibited a significant decrease in secondary and Graafian follicles while demonstrating a concomitant increase in atretic follicles (P < 0.05). Additionally, the superovulation-glyphosate group showed fewer germinal vesicle breakdown (GVBD) and MII oocytes than the superovulation group. In the superovulation-glyphosate group, there was a notable reduction in GVBD and MII oocytes following in vitro maturation (IVM).
Conclusions: Glyphosate has the potential to damage ovarian tissue and adversely affect IVM and oogenesis.
Marziyeh Pooladi, Soheila Madadi, Maryam Baazm, Azam Moslemi, Ehsan Golchini, Yusef Abbasi,
Volume 28, Issue 3 (8-2025)
Abstract
Introduction: The sinuosus canal and its branching accessory canals have crucial importance across different races for minimizing neurovascular problems in dental implantation.
Methods: This study was performed as a retrospective analysis, employing Cone beam computed tomography(CBCT) images of 174 patients who attended a private oral and maxillofacial radiology center in Arak from 2018 to 2020. The CBCT images were examined with Romexis software. Quantitative variables were reported as mean ± standard deviation, whereas qualitative variables were presented as frequency percentages. The data were analyzed using the independent T-test. The correlation between quantitative data was examined using Pearsons correlation coefficient and logistic regression analysis. One-way ANOVA was performed to analyze variables including age groups and the location of accessory canals.
Results: 65.5% of the participants had a canalis sinuosus, demonstrating a considerable prevalence of canalis sinuosus in the studied population. Age and gender did not significantly correlate with the prevalence of accessory and sinuosus canals (P > 0.05). However, a significant difference was observed in the mean diameter of the canalis sinuosus between males and females (P = 0.01). The most common location for the termination of accessory canals on the left side in females was posterior to the lateral incisor (48.3%), whereas in males, it was posterior to the central incisor (45.8%). On the right side, the most common place in females (45.5%) and males (36.4%) was also posterior to the central incisor.
Conclusions: The prevalence of sinuosus and accessory canals in different populations and races is more closely related to sample size and race and requires investigation across different races to reduce neurovascular complications.
