Hadi Ansarihadipour, Maryamsadat Alhoseini, Soheila Rostami , Narges Farahani, Mahya Hashemi ,
Volume 15, Issue 2 (June-July 2012)
Abstract
Background: The aim of this study is to assess antioxidative and pro-oxidative efficacy of ascorbate on serum albumin during iron-induced oxidative stress. Materials and Methods: In this experimental study, albumin was placed in the oxidative system containing iron ions and different concentrations of ascorbate. To monitor albumin degradation, sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed according to Laemmli procedure. Oxidative modification of albumin was demonstrated using a method for determination of carbonyl groups by 2,4-dinitrophenylhydrazine. Results: By applying the carbonyl assay, ascorbate showed a dual effect: initial pro-oxidative effect on albumin changed to an antioxidant one in a dose-dependent manner. Our findings showed prooxidant effects for ascorbate in low concentrations (0-100 µM) and antioxidant effects in higher concentrations (100-300 µM). Also, electrophoretic pattern of plasma proteins showed significant protein aggregations in the range of 35 to 45 kDa of MW and protein degradations in the range of 115 to 180 kDa. Conclusion: Ascorbate can produce reactive oxygen species and can also inhibit the production of these oxidants in the presence of iron ions as well. These findings may be directly applicable to oxidative states during the administration of ascorbate and may be important in preventing oxidative modifications of proteins in blood circulation and other biological fluids.
Elham Mohammadi, Nazanin Amini, Safoora Omidvar, Azam Moslemi, Maede Sadat Alhoseini, Faeze Vabak,
Volume 28, Issue 4 (10-2025)
Abstract
Introduction: Cataract is one of the common causes of disability in the elderly. Candidates for cataract surgery, like any other type of surgery, experience a lot of anxiety, and this anxiety has an adverse effect on their psychological, physiological and post-operative recovery. This study was conducted with the aim of investigating the effect of pre-operative education on the level of anxiety of cataract surgery candidates.
Methods: In this educational experiment, 80 patients who were candidates for cataract surgery were divided into two experimental and control groups using available sampling method. The patients in the test group received the educational intervention and the patients in the control group received the usual ward care. The anxiety levels of the two groups were determined using the Spielberger questionnaire during hospitalization, after surgery, and upon entering the ward. These levels were then compared between the two groups. The data were analyzed using descriptive statistics, Fisher's exact test, Levene's test, Chi-square test, and T-test.
Results: The results did not show a statistically significant difference between the test and control groups before the educational intervention in terms of overt anxiety (p = 0.54) and anxiety (p = 0.20), but after the implementation of the educational intervention, the results showed a statistically significant difference between the two test and control groups in terms of the mean of overt anxiety (p = 0.00) and hidden anxiety (p = 0.002).
Conclusions: The implementation of pre-operative educational intervention leads to the reduction of post-operative patients' anxiety, hence it is suggested that training be performed as a low-cost and effective method in pre-operative care.
