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Introduction: The phone's sound beams are known to be a valid test that reflects the activity of foreign hair cells, and are therefore very useful for assessing cochlear changes, such as those that occur after exposure to noise.
method: In one clinical trial, 74 volunteers aged 18-25 were examined by simple random sampling. The results of audiometric tests of the participants' pure sounds and tympanometry were within the norm on the day of the experiment. In this study, the phonetic tests of the phone were spontaneous, transient, and distorted from both ears of the subject, which was considered as a basic level of evaluation for us. Then a person's ear was selected and in the intensity of SPL 100 decibels and for 5 minutes in his ear was given a turn. After 2 minutes after the end of the presentation, the previous evaluations were repeated in both ears and the range of responses in both ears was compared.
Results: In 34 participants, submucosal nostrils led to a decrease in amplitude or SOAE responses. One of the interesting findings in this study was the emergence of new peaks in SOAE responses in the opposite ear after the presentation of the sound. The proposed noise created a significant statistical decrease in the range of TEOAE response both in the affected ear and in the opposite ear. Comparing the mean amplitude of DPOAE responses before and after exposure to shift-induced noise in the ear was significant in the Hertz range of 1699-6299, but this comparison was not significant in the opposite ear. The mean time of DPOAE incubation before and after exposure to shingles did not show a statistically significant difference in both the affected ear and the opposite ear.
Conclusion: The findings show that the phone's sound beams are a good tool for tracking hearing damage caused by noise. TEOAEs are also more sensitive to tracking than other tests, and can even be used as a very fast screening tool to assess susceptibility to hearing damage caused by noise before entering high-end industrial environments. And considered sound.

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Introduction:  Oxydative  stress may  cause  preterm  labor.  Antioxidants  found  in  fruit  and  vegetable  prevent  the  oxidative  stress  reaction.  In  this  study  we  intend  to  answer  the  question: Is  any  correlation  between  preterm  delivery  and  use  of  fruit  and  vegetable  before  and  during  pregnancy?
Materials  and  Methods:  It  is  a  case  control  study  in  which  43  women  with  preterm  delivery  and  86  women  with  term  delivery  who  delivered  in  Arak  maternity  hospital  were  selected  randomly. (sample  size  was  calculated  with  α=5%, β=20%). The  two  groups  were  matched  for  socioeconomic  status, maternal  age , gravid  and  vitamin  supplement  consumption.  Data  was  collected  with  a  questionnaire  that  includes  demographic information, and  use  of  fruit  and  vegetable (proportion  in  week )  during  the  three  month before  and  second  trimester  in  pregnancy.  SPSS software  and  K-S, t (pooled), Mann-Whitney, Chi  square  and  logistic  regression  were  used  to  analyze  the  data.
Results: This  study  showed  that  the  mean  use  of  fruit  and  vegetable  consumption  calculated  during  the  three  month  time  before  pregnancy  was  20.64±2.48  in control  group  and  32.23±1.52  in  case  group  and  the  relationship  was  significant  (p<0.00001)  and  the mean.
Use  during  the  second  trimester  was  21.84±4.75  and  34.84±5.58  in  case  and  control  groups  respectively  that  was  significant  (p<0.00001). Accuracy, sensitivity  and  specificity  of  regression  model  were  90%, 87% and  91/9%  respectively.
Conclusion: This  study  showed  that  fruit  and  vegetable  consumption  is  related  to  the  decreased  incidence  of  preterm  labor. Based on  our  model, preterm  labor  can  be  prevented  and  predicted  with  enough  consumption  of  fruit  and  vegetables.
 

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Introduction: Hyperlipidemia is one of the main known risk factors for coronary artery disease. Regarding the undesirable consequences of hyperlipidemia and its long-term treatment, it is important to use drugs with lesser complications during the long period of treatment.
Materials and Methods: This study was a double-blind randomized clinical trail that carried out on 65 patients with hyperlipidemia. Patients were divided into two groups. Case groups took dill pearl, and control group took placebo pearl for one month. Serum lipids of both groups were measured before and one month after interference. Data was analysed with t-test and p<0.05 was considered significant.
Results: In this study , differences between mean level of cholesterol, triglyseride, HDL and LDL in case group before and after interference were statistically significant (P<0/05) but in control group only difference between HDL before and after interference was significant .
Conclusion: Regarding the result of the study dill pearl can be a useful drug for treatment of hyperlipidemia.

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Introduction: As tonsillectomy operations are done in vicinity of airways, two important purposes in anesthesia are decreasing bleeding and recovery time. Because of common use of Halothane and its reported side effects, we managed a study for comparing these two factors in two methods of anesthesia with or without Halothane. Materials and Methods: In a single blinded clinical trial, 140 (4-12 years old) children undergoing tonsillectomy, were randomly allocated in two equal groups. In the control group maintenance was done with Halothane-N2O 50% but in the case group without Halothane plus hyperventilation (Liverpool technique). Bleeding volume was estimated according to preoperative and 6 hour's postoperative hematocrit. Recovery time was recorded in minutes. Data was ahalyzed using Mann-Whitney U, T, Leven and K-S tests. Results: There was not any significant difference in sex, age, and weight and operation duration in the two groups. Mean recovery time in the case group was 7.87 minutes and in the control group 15.59 minutes, which showed a significant difference (p=0.00001). Mean bleeding volume in the case group was 44.22 ml and in the control group 58.52 ml, which also showed a significant difference (p = 0.005). Conclusion: According to our study it seems that anesthesia with Halothane causes more bleeding and prolonged recovery time in comparison to Liverpool technique.

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Introduction: Preeclampsia is one of the three major causes of death in pregnant women. Researcher believed that consuming proper nutrients can be a solution. In the last few years, the effect of type of fruits and vegetables on prevention of preeclampsia has been investigated, but there are still questions about the proper amount (unit) of fruits and vegetables. Materials and Methods: This case-control study was done on pregnant women referring to Thaleghani hospital in Arak. 35 pregnant women with preeclampsia were selected as case, and 70 healthy pregnant women as control group. The two groups were matched. Data were collected with a questionnaire consisting of two parts, first demographic information and second information about the amount of consumed fruits and vegetables. Each woman was questioned about food consumption 3 month before and during second trimester of her pregnancy. Portion of fruits and vegetables was calculated per week and then compared between the two groups. K-S, Leven, Mann-Whitney, and Chi2 tests were used for analyzing data. Results: In preeclampsia group, 3 month before pregnancy mean consumption of fruits and vegetables was 7.69±16 per week, and in healthy group was 12.43±1.87. There was a significant difference between the two groups (p=0/029). In the second trimester of pregnancy the mean was 11.03±1.6 and 13.95±0.98 in case and control group, respectively. Differences were significant (p=0.03). Incidence of preeclampsia was increased in group with less than 25% consumption of fruits and vegetables before pregnancy (less than 5unit per week) (P=0.05, OR:2.32, 95%CI:1/7, 7/24). This difference was also significant in the second trimester (P<0/03, OR:2.57, 95% CI:1/4, 54/29). Also in women who consume less than 8 units per week, the incidence of preeclampsia was increased. Conclusion: It was concluded that it seems there is relationship between consumption of fruits and vegetables with decreasing occurrence of preeclampsia. So researchers suggest that high risk groups of women be identified and educated to consume fruits and vegetables.

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Background: Pain following propofol injection was reported between 28- 90 %. Ketamine have lesser cardiovascular depression effect and also local anesthetic effect. In this study effect of ketamin were analyzed when it given before propofol injection. Methods and Materials: In a randomized double blind clinical trial selected 120 ASA I, II Ptients with 15-65 years old. They were candidated for elective surgery, in half of the patients 100 µg/kg ketamine in 2 ml volume and in another half, 2 ml distilled water were given before propofol injection. Severity of injection pain was evaluated according to 4 scale criteria (none, mild, moderate, or severe) fifteen seconds after injection of 25% of the calculated dose of propofol. Heart rate (HR) and noninvasive blood pressure were recorded before and after propofol injection, immediately and 3 minutes after intubation. The data were analysed by Mann Whitney U, Pooled t-Test and the Chi-squared test. Results: Pain was not sensed in 51.56% and 75% of control and case groups respectively. In cases group showed little decrease in systolic and diastolic pressures after anesthetic induction (p=0.012 and p=0.005). There were upper diastolic pressures after intubation (p=0.00) and 3 minutes after intubation (p=0.000). During intervention heart rate changes had not significant difference between two groups. Conclusion: Ketamine pre-treatment was an effective method in reducing pain and providing hemodynamic stability after propofol induction.

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Background: Brucellosis, a zoonotic disease with worldwide spread, is a common disease in Iran and is endemic in Markazi Province. Brucella osteomyelitis, on the other hand, is a rare disease. Here we reported a case of brucella osteomyelitis of the tibia following trauma. Case: The patient was a 22 year old man referring with chronic draining tibia ulcer. He had a history of trauma of the tibia, dating back to 3 years ago, for which local prosthesis had been prescribed. Later on, the prosthesis had been removed due to infection. Staining of the tissue samples indicated the presence of gram-negative coccobacill and brucella growth in the culture of excretions. Conclusion: This case was reported because of rare presentation of brucella and the educational implications of the patient with respect to the possibility of brucella development in chronic ulcers or prosthesis. Although uncommon, brucellosis should be regarded as a potential cause of osteomyelitis in patients with a record of physical contact with animals.

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Background: Trauma noise is one of the major physical pollutants in modern societies. Sound conditioning, on the other hand, is known as one of the mechanisms for protecting the hearing system. Materials and Methods: In this experimental study, 15 three month-old male white New Zealand rabbits were divided into three groups. The equipments used in this study included a sound generator, a loudspeaker, an exposure box, a sound level meter, and a distortion product otoacoustic emission (DPOAE) device. The rabbits were assigned to trauma noise exposure, conditioning noise exposure, and control groups. The conditioning and traumatic sound levels were respectively 80 and 105dB within the 500-3000 Hz frequency. Results: Conditioning noise at the level of 80 in combination with trauma noise enhanced the rabbits hearing system tolerance up to 13-17 dB so that in all frequencies there were significant differences between the trauma noise exposure group and the sound conditioning and trauma noise conditioning group (P<0.05). Conclusion: Sound conditioning can act as an effective factor in protecting the hearing system against trauma noise.

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Background: Laryngoscopes are one of the potential mediators of infection transmission due to their blades contact with oral mucous membranes. Using single-use plastic blades is a method of preventing infection transmission. The aim of this study is to compare the efficiency of single-use plastic and reusable metal laryngoscope blades in orotracheal intubation during the rapid- sequence induction of anesthesia Materials and Methods: In this clinical-trial, 310 patients, more than 10 years of age, who were candidates for elective surgery, were selected. After anesthesia induction, orotracheal intubation was done by either single-use plastic or reusable metal blades for patients. Duration of intubation and arterial oxygen saturation were recorded before and after intubation. Data analysis was done using SPSS software. Results: Orotracheal intubation was done successfully in all patients. Mean differences of intubation time from the standard upper limit were 1.42±8.19 and 13.1±4.22 seconds in the plastic and metal blades groups, respectively. Also, the mean of difference in oxygen saturation of the low 90% after intubation were 6.07±2.71 and 7.16±1.21 in plastic and metal blades groups, respectively. Both parameters indicated statistically significant differences. Conclusion: In rapid-sequence induction of anesthesia, by using single-use blades, both intubation time and arterial oxygen saturation drop will increase in comparison with metal blades. This will cause complications such as aspiration in the patients.

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Background: Dysmenorrhea is a common problem in women of reproductive age and despite the current treatments, it still remains a common problem in women’s health. This study was done to determine the effect of aromatherapy on pain severity in primary dysmenorrhea. Materials and Methods: This randomized clinical trial was done on 108 students with primary dysmenorrhea with pain score of 2 and 3 based on the multidimensional spoken criteria. After random allocation, the participants received massage with almond oil, massage alone, and massage with a mixture of lavender and peppermint essential oil a week before menstrual period until the presence of pain in two continuous cycles. During cycles zero, one, and two, the participants recorded the highest level of pain during the first, second, and third days according to the visual analogue scale. Data were analyzed using SPSS software. Results: There was a significant difference in the total of the most pain in days 1, 2, and 3 among the groups(p=0.038). A reduction was observed in massage and aromatherapy groups(p=0.014). There were significant differences in within group comparisons (p=0.002) for zero and first cycles(p=0.016) and first and second cycles(p=0.002). Conclusion: Aromatherapy reduces menstrual pain and considering the prevalence of dysmenorrheal, the low price, and harmless nature of this essence, its application in relieving menstrual cramping and pain is recommended.

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Background: Enterococcus is known as an important pathogen in Iran like all around the world. The increasing use of vancomycin makes vancomycin-resistant enterococci (VRE) an important nosocomial pathogen. Vancomycin in combination with an aminoglycoside can provide effective treatment for severe enterococcus infections, while resistance to vancomycin antibiotic is increasing in enterococci. In this study, the pattern of antibiotic resistance and prevalence of vancomycin resistance enterococci have been explored. Materials and Methods: In this experimental study, after isolating and identifying 150 strains of enterococci from clinical specimens, the antibiotic resistance pattern of these strains to erythromycin, teicoplanin, vancomycin, ciprofloxacin, tetracycline, gentamicin, co-trimoxazole and linezolid was examined. The MIC test by using micro dilution broth method was performed for the vancomycin resistance enterococci specimens with the vancomycin and teicoplanin antibiotics. Results: Antibiotic susceptibility test showed 14% and 5.3% of the samples were resistant to vancomycin and teicoplanin respectively. Resistant to erythromycin, co-trimoxazole, ciprofloxacin, tetracycline, linezolid and gentamicin were 64, 40, 38.6, 6.6, 0, 38.76 percent respectively. Fourteen samples had high resistance to vancomycin which MIC were ≥ 256 µg/ml. Conclusion: Based on the results of present study, there are vancomycin-resistant enterococci in Arak as well as other parts of the world. The percentage of vancomycin resistance enterococci is high in Arak and appropriate treatment of infections caused by enterococcus is essential

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Background: Diabetes mellitus is one of the most important health problems in the world. B-cells are susceptible to damage by free radicals. Noticing the mechanism of cupping, this study was carried out to evaluate the effect of cupping together with drug therapy on biochemical factors and oxidative stress. Materials and Methods: In this clinical trial, 5ml of intravenous blood was obtained from diabetic patients in beginning and late stages of this disease who referred to a diabetes center. In addition to receiving metformin and glibenclamide, the patients underwent cupping after one month and were analyzed one month later. Blood samples obtained one month before and after the treatment were compared in terms of diabetes and oxidative stress indicators. To evaluate lipid peroxidation, TBA method was used and FRAPS method was employed to measure total serum antioxidants and blood factors by Pars Azmon kit. Results: Hemoglobin A1C, fasting blood sugar, blood sugar 2 hours after fasting, triglycerides, cholesterol, and low-density lipoprotein as well as high-density lipoprotein showed significant increases. Aspartate transferase in diabetic patients significantly decreased after phlebotomy (p<0.05). Conclusion: Cupping improves blood factors in diabetic patients and it is recommended to be used as a complementary treatment in patients with diabetes type II.

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Background: Brucellosis is a debilitative disease that imposes heavy costs on the economy and society. Therefore, using the most accurate and efficient method to diagnose this disease is essential. In Iran, Brucella melitensis is the common causative agent for brucellosis and BP26 protein of this bacterium has a good level of antigenicity. Thus, the aim of this study is to produce Brucella melitensis recombinant BP26 protein with a PET28a expression vector.

Materials and Methods: In this applied-fundamental study, genomic DNA was isolated from bacterial culture through proteinase K (pK) and phenol/chlorophorm protocol. Then, two pairs of primers were designed based on the known sequence in the gene bank for amplification of Brucella melitensis bp26 gene and PCR reaction was set up and optimized. The PCR product was cloned first into PTZ57R/T vector and accessed on the PET28a vector and sequenced. The recombinant vector was transformed and expressed into E. coli BL21 (DE3). Then, the recombinant protein was purified with Ni-NTA column of chromatography against His tag.

Results: The size of PCR product was in accordance with the part of bp26 gene size in the gene bank. The bp26 gene without adding IPTG had little expression and 3 hours after adding IPTG with a 1 Mm concentration to culture media, extreme expression was observed.

Conclusion: The production of recombinant BP26 protein from isolated Brucella melitensis native to Markazi province was done. Noticing the importance of BP26 protein and its significance for future studies on providing brucellosis diagnosis kits, its production was made possible.

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Background: Genital tract infections are one of the main causes of frequent referrals to gynecological clinics. Vulvovaginal candidiasis is the second common infectious vaginitis. On the other hand, probiotics are microorganisms that can have beneficial effects on the host. Noticing the limited number of studies reporting new treatments for this disease, the present study was designed to compare the effects of the combination of fluconazole and oral protexin and fluconazole on the treatment of vulvovaginal candidiasis.

Materials and Methods: A double-blind clinical trial was conducted on 90 women who were referred to the 12-e-Bahman Clinic in 2011. The patients were randomly classified into "combination of fluconazole and oral protexin" or "fluconazole and placebo" groups. Data were analyzed by descriptive statistics and inferential statistics (t-test, Chi-square test, Fisher’s exact test, and McNemar’s test).

Results: The combinations, fluconazole-oral protexin and fluconazole-placebo, were equally effective in reduction of complaints and symptoms however, the effect of fluconazole-oral protexin on dysuria was more (p=0.02). Also, the fluconazole-oral protexin combination elicited a better therapeutic response (p=0.01). In addition, fluconazole-oral protexin combination treatment demonstrated a better recovery time (p=0.04).

Conclusion: This study demonstrated that complementary treatment with probiotic lactobacillus increased the efficacy of fluconazole in treatment of vulvovaginal candidiasis. Further research, however, is suggested.

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Background: Depression is one of the most common problems in postmenopausal women. Diosgenin present in fenugreek seeds present study the effects of menopause on depression in menopausal women took the city in 1390.

Materials and Methods: In a randomized controlled trial enrolled 60 women with complaints of depression caused by menopause. Beck Depression Inventory on a sample of postmenopausal women and the inclusion criteria were enrolled. The samples were divided randomly into two groups, Diosgenin (6 g Fenugreek seed daily containing 25 mg Diosgenin) and sorbitol (25 mg daily) for 8 weeks were placed before and after 8 weeks of intervention questionnaire, Beck completed SPSS software was used to test the statistical Paired test scores before and after treatment were compared.

Results: Mean depression score decreased after 8 weeks took Fenugreek containing Diosgenin and sorbitol as compared with before of intervention of this study and it was significant.The comparison did not indicate a significant difference between the two groups.

Conclusion: According to the finding of this study, administration of 6 g Fenugreek seed containing Diosgenin for 8 weeks decreased menopausal depression . The need for further studies with longer duration and higher doses before any recommendations can be felt.

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Background: According to the studies, Iran’s per capita antibiotic consumption is remarkable. Since the emergence of Antibiotic Resistant Organisms (AROs) is considered a global problem in the community and hospitals, this study aimed to investigate the pattern of antibiotic resistance in Gram-negative bacteria isolated from patients in an academic and a non-academic hospital in Arak, Iran.

Materials and Methods: This descriptive cross-sectional study included all specimens referred to the microbiology laboratory at Amir Al-Momenin Hospital (academic hospital) and Imam Khomeini Hospital (non-academic hospital) in a one-year period from June 2012 to June 2013. The susceptibility of the isolated Gram-negative bacteria against the important antibiotics used in clinical practice was determined using the disk diffusion method.

Results: A total of 846 specimens were collected from patients in the two hospitals where 520 (61.5%) were Gram-negative bacteria, of which 63.3% were E. coli. The prevalence of Gram-negative organisms in the academic and the non-academic hospital was measured 79.8% and 20.2%, respectively (p=0.097). It was also determined that there is a significant difference between the two hospitals in the prevalence of ESBL (Extended-Spectrum Beta-Lactamase) Gram-negative bacteria (p=0.003).

Conclusion: There was a significant difference between the two hospitals in the prevalence of ESBL Gram-negative bacteria. Therefore, conducting regional surveillance programs to examine the patterns of antibiotic resistance is recommended.

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Background: The pathogenesis of recurrent pregnancy loss includes complex interaction of several genetic and environmental factors. Changes in blood coagulation factors during pregnancy may play an important role in the occurrence of recurrent abortions (RA). Recently, inherited thrombophilia has been considered as a possible cause. Therefore, in this study we have investigated association of factor V (G1691A) and factor II (G20210A) polymorphisms in Iranian patients with recurrent abortions.

Materials and Methods: A total of 203 women participated in this study: 105 women with two or more consecutive unexplained miscarriage as cases and 98 women with at least two healthy children as control group. Total genomic DNA was isolated from Peripheral blood leukocytes. The presence or absence of mutation in the FV (G1691A) and FII (G20210A) polymorphisms were assessed by PCR-RFLP, using Mnl1 and HindIII digestion enzymes, respectively. Finally, the data were  analyzed using Chi-Square test.

Results: The results showed no statistical significant differences in the prevalence of FV (G1691A) and FII (G20210A) polymorphisms between patients and control group.

Conclusion: considering the results of this study, these polymorphisms Seem to have no role in etiology of recurrent pregnancy loss in the studied population.

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Background: There have been numerous reports of snake venoms being employed as analgesics in attempts to relieve severe pain associated with cancer, immune dysfunction and viral infections. This study investigates the antinociceptive effects of iranian cobra snake venom (Naja naja oxiana) in comparison with morphine and lidocain on laboratorial femal mice.

Materials and Methods: This study has been done on 48 NMRI female mice of 18-20 g in weight. Antinociceptive activeity of snake venom was evaluated by formalin test. In this test, the animals were divided into 6 groups (each group consisting of 8 mice): Sham, positive Control (receiving morphine at dose of 5 mg/kg, and receiving lidocain at dose of 20 mg/kg), and experimental groups receiving venom at doses of 1, 3 and 4.5 µg/mice. In all groups, the formalin test was recorded for 60 min after administration of venom and drugs in mice. Data were analyzed using one-way ANOVA and Tukey test.

Results: The results showed that the venom of Naja naja oxiana decreased nociception meaningfully in both acute and chronic phases. We also showed that this venom revealed even a better analgesic activity in comparison with morphine and lidocain.

Conclusion: This study showed that the antinociceptive effect of the venom was mediated through central nervous system and peripheral mechanisms. Although details of the mechanism remain unclear, and further studies should be considered to demonstrate its therapeutic effects.

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Abstract

Background: Probiotics are known as a factor for prevention of infectious diseases such as urinary tract infection(UTI). The present study aimed to investigate the positive effects of probiotics on urinery truct infection of cateterized intensive care unit patients.

Materials and Methods: In a triple blind clinical trial, 125 cateterized intensive care unit patients were randomly selected and divided into two groups, Treatment group (n = 63) was well matched with placebo group (n = 62) for age and sex and duration of admission .Case group received one lactocare capsule for 10 days and control group with a same method received placebo for 10 days. In both groups urine analysis and urine culture were measured with a same method at the first day and then twentieth day and finally data were analysed with t-test.

Results: The incidence of urinary tract infection between treatment and control group (respectively 17.4% and 22.5%) had no statistically significant difference (p=0.672). But, in terms of sex, incidence of urinary tract infections in women compared to men with the same condition was significantly different(p=0.002). In treatment group with long term cathetrization, urinary tract infections significantly were significantly different(p=0.041).

Conclusion: Administration of lactocare capsule has no statistically significant impact on the incidence of urinery truct infection of cateterized intensive care unit patients. In comparsion of cateterized men and women receiving probiotics, the reduction of urinary tract infection in women was more than in men. Probiotics had preventive effects on long term catheterization.

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Abstract

Background: Family is the most important component of community and family health leads to spiritual excellence and intellectual growth. One of the debates in current society is to enhance the bases of marital life. More than half of the sexual problems which cause family life and marital relationships abolishment are due to insufficient knowledge or erroneous beliefs about sexual relationships. The aim of this study is to investigate the effect of acceptance commitment therapy (ACT)-based premarital counseling on the couples’ sexual function.

Materials and Methods: The study population of this clinical trial consisted of all couples referring to premarital counseling centers of Isfahan in 2015. Sixty couples were selected and then randomly divided into two groups, an experimental group(30 couples) and a control group(30 couples). Then they completed a questionnaire of sexual function. The data were analyzed by SPSS 21 using the Mann-Whitney test.

Conclusion: The results of this study with the aforementioned condition showed the lack of significant difference in the couple’s sexual function between premarital counseling and the approach based on acceptance and commitment. So, it seems that performing a research with more samples and a target group having 1-5 marital experience would be necessary.

Results: The results showed no significant difference between the effect of premarital counseling acceptance and commitment therapy – based approach on the couples’ sexual fanction in comparison with a premarital conventional approach(p>0.02).