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Introduction:The membranes used in haemodialysis may be manufactured from cellulose, modified cellulose or synthetic polymers. Such membranes, when in contact with blood will activate the complement system, which entails changes in leukocyte and platelet counts. Polysulfone is a synthetic membrane of high biocompatibility standards, whereas haemophane membranes are modified cellulose-based membranes. The biocompatible profiles of these membranes, has been studied by clinical reactions (i.e. hypotension, nausea, pruritis, …) during dialysis sessions. Both kinds of these membranes are used in Iran. The number and severity of these reactions define the degree of dialysis biocompatibility. Materials and Methods: In a clinical trial study which was carried out in Imam Khomeini hospital in Tehran, 100 hemodialysis patients were enrolled to this investigation. Their clinical reactions were compared during 3 sessions of hemodialysis with polysulfone and 3 sessions of hemodialysis with hemophan membrane. Each patient was the control of him/herself. Data was analyzed using Chi square test. Results:Mean age of patients was 48.85±17.56 years and 39% of them were female. The most common complications were hypotension and muscle cramps (each 21.5%). Hypotension was higher in sessions of polysulfon versus hemophane (18% versus 25%) but was not significantly different. Also other complications such as muscle cramp, nausea, vomiting, dyspnea, headache, chills and fever had no significant difference. Seizure did not occur in any patient. Conclusion: According to our findings the membrane’s type has no role in acute clinical complications during hemodialysis and in most patients, membranes can be used according to their availability. It seems their biocompatibility has no considerable clinical difference.

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Introduction: Children with reflux who fail prophylaxis management or reflux which do not resolve spontaneously are candidates for sugery. Delay in surgery increases the development of new renal scars. Finding a modal factor for prediction of reflux resolution likelihood can decrease complications of treatments. Regarding that sacral bone anomalies have a direct influence on the final function of urinary and bowel systems, this study is done to investigate the relationship between sacral ratio and reflux. Materials and Methods: This is a case-control study. The case group included 76 children less than 9 years old refered to Vali-e-Asr and Amir Kabir hospital clinics with urinary tract infection and according to voiding cystouretrograply (VCUG) results, children with III, IV and V grade vesicoureteral reflux (VUR) entered the study. The control group included 76 children with previous urinary tract infection and normal VCUG. Sacral ratio in the two groups was measured and was compared by student t, Chi square and Mann Whitney tests. Results: The case group included 24 males and 52 females with average age of 2.7 years old and the control group included 23 males and 53 females with the average age of 3 years. The percent of sacral ratio abnormality were 40% and 23.7% in the case and control groups respectively, which was significantly different (p=0.001). The difference between the mean sacral ratio in case group with the value of 80% and the control group with the value of 84% was not different. The ratio distribution pattern in the two groups was not different. Conclusion: The percent of sacral ratio abnormality in children with vesicoureteral reflux were more than children without reflux.

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Background: Hospital readmission rate as a global health problem has increased over the last few decades. Congestive heart failure (CHF) is one of the most common causes of hospital readmission. Little is known about barriers of readmission rate control. The aim of this study was used for data exploring the barriers of readmission rate control. Methods and Materials: A qualitative study was designed with grounded theory approach. Data were gathered with interview. This study was started with proposly sampling and continues with theoretical sampling during 6 months. A convenience sample of 42 people was recruited from two public educational hospitals in Tehran during 6 month. The data were analyzed using constant comparative analysis. Results: Data analysis demonstrated lake of sensitivity on barriers of readmission incorrect patient’s health believes and expectations insufficient patient education, drug and diet adherence, and incorrect life style lack of active medical system , distrust on physicians and lack of communication psychological issues and patient - family challenges, were as the barriers of readmission control. Conclusion: This research had shown that the barriers of readmission control recognized by applying Qualitative research method with source triangulation better than the biomedical approach with single source. On the other hand, lack of sensitivity of partcipants on barriers of readmission control is a new finding. So, for controlling the barriers of readmission despite of exploring the barriers, making sensitive of involved people to readmission, is also proposed

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Background: Candida species are the most common yeasts that reside in oral cavity in 30 - 50% of people, and are capable to produce opportunistic infections within the oral cavity. This study was done to determine the effect of cigarette smoking on the type and degree of Candida oral carriage. Materials and Methods: In this case-control study, oral Candida flora in 34 smoker and 66 non-smoker men were compared. All of the samples were collected by swab and examined by direct microscopy and culture on CHOROM agar Candida and corn meal agar media. Isolated colonies were identified by carbohydrate assimilation method using RapID Yeast PLUS System. Results: Out of the 34 smoker individuals, 27(79.4%) had Candida colonization in their oral cavity while 30(45.5%) of the 66 non-smoker individuals had developed this kind of colonization. A significant difference was observed between the two groups (p<0.05). Additionally, there was a significant difference in the degree of colonisation and the type of candida species which were isolated from the oral cavity of the smoker and nonsmoker groups. Conclusion: Cigarette smoking can act as an important underlying factor in oral candidiasis.

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Background: Recently, reports have been made of the effects of boric acid (BA) on cancer prevention and inhibition of cancer cell proliferation. This study was designed to investigate the effects of this compound on K562 cell line as a model of chronic myeloid leukemia (CML). Materials and Methods: In this experimental trial, K562 cell line was cultured in the presence of 0.75 to 12 mmol concentrations of boric acid for 24, 48, 72, and 96 hour intervals. Anti-proliferative and cytotoxic effects of BA were measured by trypan blue exclusion test and MTT assay, respectively. Flow-cytometery was utilized for evaluating the effects of BA on cell cycle. Wright-giemsa staining was used for determining the effects of BA, and latex phagocytic assay was used for evaluating the phagocytic potential of the differentiated cells. Results: BA induced growth inhibition of K562 cells in a dose and time dependent manner after 96 hours of treatment with 12 mmol BA, cell proliferation of K562 cells was inhibited to about 83% (p<0.001). In addition, BA induced G1 cell cycle arrest in a way that for instance, after 6 days of treatment with 9 mmol BA, 98% of cell populations were at G1 level. Wright-giemsa staining and latex phagocytic assay results confirmed that K562 cells differentiated toward monocyte-macrophage lineage. Conclusion: Noticing the anti-proliferative and differentiating effects of BA, and no evidence of its adverse effects, this compound can be used as alone or in combination with other drugs in CML differentiation therapy.

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Dermatoglyphic patterns are formed during the first and the second fetal development trimesters and they remain unchanged afterwards. At the end of the fourth month of fetal development, fingertips are formed along with brain and other derived ectodermia developments. In the 19th century, the mystery of human fingertips was discovered, and it was realized that fingertips consist of tiny network lines in the epiderms of the skin, and are exclusive to every individual, even to twins. In this study, through library research, by reviewing quantitative and qualitative dermatoglyphic patterns, the miracle of fingertips was considered based on Quran and Islamic commandments. Much research has been done comparing individual dermatoglyphic patterns of hands among various races, and the quantitative and qualitative relationship between dermatoglyphic patterns and diseases such as schizophrenia, nervous disorders, Down syndrome, diabetes I, Alzheimer, multiple sclerosis, congenital spinal abnormalities, and psoriasis disease has been shown. Accordingly, finger prints are used for identifying individuals, detecting criminals, and studying disease of genetic background. They can also be used as clues in determining the etiology of diseases. Fingertip sign is unique for every individual. Quran, in response to those who deny the resurrection after decomposition of the body and bones (Saffat, 37: 16), declares that Allah almighty, is not only capable of recreating them, but also is able to make human fingertips again (75: 3-4). The study shows the importance of human’s fingertips as the miracle of human creation, and reveals why Quran emphasizes on them as one indication of resurrection.

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As the world is moving at the fastest possible speed toward industrialization, great technical developments, and becoming a place devoid of difficulties and troubles, the issue of illnesses and mental problems still persists as an unpleasant matter which is quite noticeable. This study was done through reviewing the available Quranic books and resources. In such matters, Quran and Hadith are rich in both content and foundation and play a vital role in decreasing and alleviating mental pressures and enhancing and establishing mental health in the society. In this regard, Islam (Quran and Hadith) enjoys and offers recommendations and solutions for fortifying the individual’s mental and spiritual basis in the society in a way that it eliminates the peoples’ economic and mental pressures and strengthens or establishes their economic mental hygiene. In this regard, the solutions and recommendations of Quran and Hadith can be applied to the treatment of the majority of economic mental diseases and their consequences.

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Background: Some plant extracts, including species of Santolina have antibacterial effects and they can be used as antimicrobial agents in treatment of infections. Hence, the aim of this study was to evaluate the compounds of essential oil and the anti-microbial properties of its essential oil and extract. Materials and Methods: In this experimental study, yarrow plant in late spring was collected from Sistan region in 2008. The compounds of the essential oil were analyzed by GC/MS. In this study, the minimum inhibitory concentration (MIC) and diameter of inhibition zone of growth for the standard strains of Staphylococcus aureus, E.coli, P.aeruginosa, and Candida.albicans were determined through disk diffusion and agar-well diffusion methods and dilution in the liquid medium, respectively. Results: Camphor was the major compound of the essential oil. The standard strains of Staphylococcus aureus presented the greatest sensitivity to the stem extract and leaf extract in MIC> 0.573 and MBC> 1.146, respectively and to the flower extract in MBC> 1.663 and MIC> 0.831, respectively. In addition, it presented an intermediate sensitivity to standard strains E.coli with MBC> 2.293 and MIC> 1.146, respectively to the stem and leaf extract and MBC> 6.650 and MIC> 3.325 respectively to the flower extract. However, the standard strains of Candida albicans and P.aeruginosa did not show a significant sensitivity to the extracts. Also, the essential oil of this plant in comparison with the extracts did not have any significant antimicrobial effects. Conclusion: The plant extracts, especially stem and leaf possess anti-bacterial effects. But further investigations are needed for determining its exact mechanism

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Background: Differentiation of M. tuberculosis complex organisms were assigned to one of three genotypic groups based on the combinations of polymorphisms at katG codon 463 and gyrA codon 95. Early identification of strains belonging to any particular group is very important. This study was planned to identify major genetic groups of clinically isolated Mycobacterium tuberculosis. Materials and Methods: In this cross sectional study 33 sputum samples were collected from tuberculosis patients of the Markazi province. DNA purification from isolated samples was performed by Chelex 100. Identification of isolates was confirmed by detection of katG gene and the mutation in KatG463 by using PCR method and RFLP respectively. Finally 620-bp of katG gene and 194-bp of gyrA gene purified from PCR product were sequenced. Results: Amplification of 620-bp fragment of katG gene was a good way to confirm the detection of bacteria as a molecular approach. Results of sequencing codon GyrA95 in combination by results of PCR-RFLP determined type of the major genetic group (MGG). Therefore it showed that among the 33 Mycobacterium tuberculosis isolates 12 samples were MGG 1, 15 Samples were MGG2 and 6 samples were MGG 3. Results revealed that MGG 2 was dominant form of M. tuberculosis strains of Markazi province by frequency of 45.5%. Conclusion: Based on the results of this study MGG2 occurrence was more frequent among clinical strains in Markazi province that its accordance with susceptibility of these strains to conventional antibiotics is notable. In this study, three applicable benefits from the test as: MGG typing, molecular detection of M. tuberculosis and bacterial resistance to Isoniazid were proven.

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Background: Deaths due to cancer have been increasing in many countries in recent years. Probiotics are live microorganisms that have beneficial effects on consumer’s health. The aim of this study was to investigate antimutagenic and anticancer effects of Lactobacilli strains isolated from Tarkhineh in order to select potential probiotic strains. Materials and Methods: In this experimental study, 25 strains of Lactobacillus that had previously been isolated from Tarkhineh were used. Antimutagenicity test was performed based on proposed method of Ames. The results of the test were individuated after comparison between samples (suspensions of Lactobacilli) and positive (sodium azide) and negative (distilled water) controls. SPSS version 16 was used for statistical analysis. Results: Antimutagenic activity was observed only in four strains of Lactobacilli. The inhibitory percentage in the four strains of Lactobacilli, in its highest level was equal to 60.38 and in its lowest percentage equal to 39.37%. Also, the inhibitory percentage of mutation among the four strains in the presence of microsome were significantly different (P<0.05) than the absence of microsome. Adding the microsome of rat liver (S9) proved the anticancer effects of four Lactobacilli strains. Conclusion: Of the 25 strains of Lactobacilli, 4 strains which had the highest antimutagenicity effects were chosen as the potential probiotic strains.

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Background: Occult hepatitis C virus (HCV) infection is defined as the presence of HCV-RNA in liver or peripheral blood mononuclear cells (PBMCs) in the absence of detectable hepatitis C antibody (anti-HCV) or HCV-RNA in the serum. Although occult HCV infection is less aggressive than classic chronic HCV infection, nevertheless it is important in management of hemodialysis (HD) patients. Occult HCV infection has been described in two different clinical settings: in patients with normal liver enzymes or in patients with abnormal values of liver enzymes. The aim of this study was to detect the occult HCV infection in hemodialysis patients with elevated liver enzymes.

Materials and Methods: This cross sectional study was performed in hemodialysis patients referring to 3 dialysis units in Tehran. In 30 anti-HCV negative HD patients with elevated liver enzymes, presence of HCV-RNA in plasma samples was tested by Reverse Transcriptase-Nested Polymerase Chain Reaction (RT-nested PCR). In cases with negative anti-HCV and plasma HCV-RNA, HCV-RNA was checked in PBMC specimens by RT-nested PCR.

Results: A total of 30 HD patients with mean age 54.4± 14.1years and mean dialysis duration 81.2 ±64.4 months were enrolled in the study. All HD subjects were negative for HCV-RNA in plasma and occult HCV infection with RT-nested PCR method.

Conclusion: Occult HCV infection was rare in HD patients with elevated levels of liver enzymes.

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Background: Cotonary artery illneses are the common heart illness that there is treatment methods now, many patients need the coronary artery bypass graft. Anxiety is a common phenomenon after all surgical operation. Anxiety in coronary artery bypass graft causes to increase metabolic rate, decrease immunity of body, and increases activity of heart coronary and gastrointestinal. This study was perfound to assess the effects of metatarsus foot reflexology on state anxiety levels after coronary artery bypass graft in Imam Ali Hospital Kermanshah.

Methods and Materials: This clinical trial included 88 patients that they were divided in to the intervention and control group based on the randomly. The intervention group received a 10 minute right foot massage one day on third and fourth day after coronary artery bypass graft and control group received no intervention, only at the time mentioned, score anxiety was measured with standard questionnaire spielberger.

Results: Patients state anxiety significanty decreased in the intervention group in the third day comparison with the control group, but no difference were not observed in the control group.

Conclusion: The finding of the study showed foot reflexology to be effective and simple that complication and damages are low that is used in reducing patients anxiety in treatment-health centers.

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Background: Pediatricians frequently confront with elevated body temperature in children and subsequent anxious parents. They practice several approaches in management of fever. A recently addressed issue is administration of alternating doses of acetaminophen and Ibuprofen. This method is relatively common , despite lack of sufficient evidence in this field.

Materials and Methods: This study is a randomized clinical trial. A total of 240 children, aged 6 months to 12 years suffering from gastroenteritis was recruited in this research. Children were classified in 3 groups. First group received Acetaminophen, second group received Ibuprofen and third group were treated with acetaminophen – Ibuprofen every other day alternately.

Results: 240 child were taken apart in this research . 112 ones were female (46.66%) and 128 were male (53.33%). The mean time of lowering fever in the first group was 2.07 days. This figure in the second and third groups were 1.82 and 1.87 respectively. Average doses in the first, second and third groups were (in order) 9, 8.17 and 7.13 doses. Medication in the first group minimum effectiveness rather than two other groups.

Conclusion: The alternating regimen of Acetaminophen – Ibuprofen is more effective than monotherapy in reduction of fever in infants and children between 6 months to 12 years.

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Background: Considering the increased activity of hypothalamic orexinergic neurons due to morphine administration, and its extensive projections to the hippocampus, it is probable that morphine effect on CA1 neuronal function is mediated by orexinergic system. So the effect of hippocampal orexin-1 receptors (OX1R) blockade on CA1 baseline synaptic response and short term synaptic plasticity was investigated.

Materials and Methods: In this experimental study, animals received morphine 10 mg/kg/12h/(SC) for 10 days. SB-334867-A, OX1R antagonist (0.5&mug/0.5 &mul), was microinjected intrahippcampally for OX1R inhibition before each morphine injection. Baseline synaptic response and short term synaptic plasticity were evaluated by field potential recording. fEPSP was recorded from CA1 following Schaffer collaterals stimulation. After Input/Output construction, short term synaptic plasticity was induced by paired pulse stimulations.

Results: Chronic use of morphine did not affect the baseline synaptic response (p>0.05). SB- 334867-A microinjection in CA1 did not have any effect on baseline synaptic response in morphine dependent rats. Morphine increased paired pulse index (PPI) at 80 ms inter pulse interval (IPI, p<0.05). SB-334867-A pretreatment did not affect this morphine induced PPI change.

Conclusion: The results suggest that orexin-1 receptors (OX1R) do not mediate the effect of morphine on baseline synaptic response and short term synaptic plasticity in CA1 area of the hippocampus.

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Background: Some children have abrupt onset of severe urinary frequency, voiding as often as every 10-15 min during the day, without dysuria, UTI, daytime incontinence, or nocturia. The most common age for these symptoms to occur is 4-6 yr, after the child is toilet trained, and the vast majority are boys. This condition is termed the daytime frequency syndrome of childhood or Pollakiuria. The condition is functional no anatomic problem is detected. The symptoms occur often just before a child starts kindergarten or if the child is having emotional family stress-related problems. OCD is a chronically disabling illness characterized by repetitive, ritualistic behaviors over which the patient has little or no control. OCD has a lifetime prevalence of 1-3% worldwide, and as many as 80% of all cases have their onset in childhood and adolescence. The purpose of this study was to investigate the relationship between OCD and Pollakiuria.

Materials and Methods:In this case-control study, we evaluated (152) children aged 6-18 years old who were visited in the pediatric clinics of Amir-Kabir Hospital, Arak, Iran. The control group considered of (76) healthy children and the case group included (76) age and sex matched children with Pollakiuria. Then, the children’s behavioral status was evaluated using the children’s Yale-Brown Obsessive-Compulsive Scale (C-YBOCS). The C-YBOCS is helpful in identifying children with OCD. The data was analyzed using descriptive and analytical statistics in SPSS-16.

Results: OCD was detected in (5) case (6.6%) with Pollakiuria and (8) controls (10.5%). The difference in OCD was not significant (p-value 0.282) between the two groups.

Conclusion: OCD isn’t more common in Pollakiuria versus non-Pollakiuria children. It is recommended to conduct a study with higher sample volume in order to detect the relationship between OCD and Pollakiuria.

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Background: This study aimed to examine the antibacterial effect of Polyamidoamine-G4 dendrimer on Escherichia coli, Enterobacter cloacae, Bacillus subtilis and Staphylococcus aureus.

Materials and Methods: In this experimental study, the antibacterial effects of Polyamidoamine-G4 dendrimer were studied by disc diffusion and micro-dilution method. Different concentrations of Polyamidoamine-G4 inoculated onto blank disks and were placed in Mueller-Hinton agar media. Zone of inhibition was investigated by bacterial inoculation according to the McFarland standard 0.5. Minimum inhibitory concentration and minimum bactericidal concentration of Polyamidoamine-G4 dendrimer were determined by micro-dilution method in nutrient broth culture.

Results: Zone of inhibition in concentration 500 &mug/ml of Polyamidoamine-G4 dendrimers for Escherichia coli, Enterobacter cloacae, Bacillus subtilis and Staphylococcus aureus were 14, 0, 35 and 29mm, respectively. Concerning the Zone of inhibition in gram negative bacteria with gram positive ones was p<0.05 and had significant difference. The minimum inhibitory concentration of Polyamidoamine-G4 dendrimer for Escherichia coli, Bacillus subtilis and Staphylococcus aureus were 1250, 2.5, and 1 &mug/ml, respectively. The minimum bactericidal concentration of Polyamidoamine-G4 dendrimer belonged to Escherichia coli, Bacillus subtilis and Staphylococcus aureus were 2500, 5 and 5 &mug/ml, respectively. Polyamidoamine-G4 dendrimer had not bacteriostatic and bactericidal effects on Enterobacter cloacae.

Conclusion: According to the results, Polyamidoamine-G4 dendrimer can eliminate Escherichia coli, Staphylococcus aureus and Bacillus subtilis effectively. It is suggested in the rest of this study that the probable toxicity of nanostructured compounds examined in drinking water and, economic studies is done for synthesis and their applications in case of prevention of using.

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Background: Male fertility depends on the proper function of a complex system of organs which plays an important role in spermatogenesis. In this study the effects of sulpiride-administration were assessed by means of sperm parameters and in vitro fertilization potential.

Materials and Methods: In this experimental study thirty adult male mice were divided into 3 groups as test, control-sham and control. The test group were injected with 40 mg/kg sulpiride solution daily for 45 days IP. Sham mice were injected by solvent only. After 45 days, all mice were dispatched by cervical dislocation consequence of unconsciousness. Cauda epididymis were used to collect sperm cells and assess their motility, viability and DNA integrity. The rate of in vitro fertilization and embryonic development were also examined.

Results: In comparison with sham and control groups, sperm motility and viability rate showed a significant reduction in the sulpiride-administered animals. Rate of DNA damage increased which gives rise to a remarkable reduction of fertilization rate, zygote division, blastocysts number, and significant increase of arrested embryos in sulpiride treated mice (p<0.05).

Conclusion: Data suggest that following sulpiride-induced hyperprolactinemia, induction of spermatogenesis dysfunction, causes low sperm quality that accompanies a significant lower fertility potential and embryonic development in comparison with the sham and control groups.

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Background: One of the side effects of chemotherapy drugs is oxidative stress that can damage the sperm and decrease fertility potential. Antioxidant agents in Imedeen like Lycophence GS and Biomarine complex play important role in preventing the direct and indirect effects of free radicals. So, in this study, the inhibitory effects of Imedeen on the damage caused by cyclophosphamide were investigated.

Materials and Methods: In this experimental study, 60 mature male mice were divided into six groups. The control group received physiological serum, the second group received CP with 12mg/kg/day dosage, the third group received Imedeen with 111µg/kg/day dosage, the fourth group received Imedeen with 222 µg/kg/day dosage, the fifth group received CP and Imedeen with one dosage and the last group received CP and Imedeen with double dosage. Sampling and studies on sperm quality were performed after 35 days.

Results: The results obtained from the caudal epididymal sperm analysis revealed that treated with CP caused significant decrease in sperm count, motility, and viability, while abnormal sperms increased as compared to control gruop. These changes were associated with significant increase in DNA damage and chromatin abnormality in the caudal epididymal spermatozoa as evidenced by Acridine Orange and Aniline Blue staining respectively. Notably administration of Imedeen caused a considerable recovery in above-mentioned parameters.

Conclusion: The results suggest that Imedeen as an antioxidant could diminish the side effects of cyclophosphamide in the reproductive system of male mice.