Journal of Arak University of Medical Sciences

New Protein Expression of P39 Compound on Vesla Abortus in Escherichia Coli Bacteria

Hamid Abtahi

Introduction: Brucellosis is one of the most important zoonotic diseases that causes miscarriage and infertility in animals and causes human fever. The use of the common SS9 strain of Brucella abortus has several side effects for livestock. Brucella P39 protein is one of the plasma peripheral space proteins that is considered as one of the important immunogenic indicators. With the production of the new protein combination of P39, more studies can be done on the ability of this protein to stimulate immune responses against Brucella. Therefore, in this research, the production and purification of this protein in Escherichia coli bacteria has been done as a new compound.
method: In this experimental study, using the polymerase chain reaction, the P39 gene was propagated by the bacterium Brucella abortus. After purifying the P39 gene, it was cloned into plasmid carriers pSK+ and pGEX4T1. Therefore, pSK+-P39 and pGEX4T1-P39 structures were prepared. To produce the recombinant protein P39, the plasmid structure pGEX4T1-P39 first entered the Escherichia coli bacterium BL21. The protein was then produced by IPTG by induction of pGEX4T1-P39 plasmid. The resulting protein was purified using the orderly purification protein glutathione S-transferase. The amount of purified protein was measured using the Brad Ford method.
Results: The nucleotide sequence of the gene propagated by the cloned PCR in the plasmid carrier pSK+ was exactly the same as the P39 gene of Brucella abortus. Production of P39 protein was performed by induction of pGEX4T1-P39 plasmid. The purified protein content was 200 micrograms per milliliter.
Conclusion: The production of the new protein P39 compound Brucella Abortus, which is unstable in the cytoplasm of the Escherichia coli bacterium, is possible using carriers with additive proteins such as pGEX4T1 in the host of Escherichia coli strain BL21.

Use of External Stabilizer in the Treatment of Radius End Fractures

Mohammad Reza Etemadifar

Introduction: Radius end fracture is one of the most common fractures of the upper limb, the treatment of which is controversial, and improper treatment can have significant side effects. In this study, we intend to examine the results of treatment with an external fixative method.
method: It was easy to study without control and sampling. The study was performed on 30 fractures (26 patients) for one year, based on radiographs of the fracture site of type 4 universal 5. Surgery with standard techniques was performed under general or regional anesthesia, and patients were followed up for up to one year after surgery and examined for shortness of breath, joint surface angle, range of motion, and pain.
Results: Twenty-four men and two women with a mean age of 5.28 years were examined. In all patients, clinical and radiographic effusion occurred. There were two cases of mild infection at the site of the pin, which were treated with medication. In one case, there was a slight sensation in the sensory branch of the radial nerve, which disappeared after a while, and in no case was there a tendon lesion. The mean deviation to the palm was 6.6.5.5 and the ulnar deviation was 4.5 to 12.5.
There were 4 cases of pain during strenuous activity, 2 cases of mild movement restriction and one case of severe movement restriction.
Conclusion: Compared to other studies, the results of this study are much better in many cases and it is recommended to use this treatment in the treatment of unstable fractures of the lower end of the radius and especially if it develops into the radiocarpal joint.

Tracking Hearing Damage Caused by Noise Using the Phone's Sound Beam Test

Arash Bayat

Introduction: The phone's sound beams are known to be a valid test that reflects the activity of foreign hair cells, and are therefore very useful for assessing cochlear changes, such as those that occur after exposure to noise.
method: In one clinical trial, 74 volunteers aged 18-25 were examined by simple random sampling. The results of audiometric tests of the participants' pure sounds and tympanometry were within the norm on the day of the experiment. In this study, the phonetic tests of the phone were spontaneous, transient, and distorted from both ears of the subject, which was considered as a basic level of evaluation for us. Then a person's ear was selected and in the intensity of SPL 100 decibels and for 5 minutes in his ear was given a turn. After 2 minutes after the end of the presentation, the previous evaluations were repeated in both ears and the range of responses in both ears was compared.
Results: In 34 participants, submucosal nostrils led to a decrease in amplitude or SOAE responses. One of the interesting findings in this study was the emergence of new peaks in SOAE responses in the opposite ear after the presentation of the sound. The proposed noise created a significant statistical decrease in the range of TEOAE response both in the affected ear and in the opposite ear. Comparing the mean amplitude of DPOAE responses before and after exposure to shift-induced noise in the ear was significant in the Hertz range of 1699-6299, but this comparison was not significant in the opposite ear. The mean time of DPOAE incubation before and after exposure to shingles did not show a statistically significant difference in both the affected ear and the opposite ear.
Conclusion: The findings show that the phone's sound beams are a good tool for tracking hearing damage caused by noise. TEOAEs are also more sensitive to tracking than other tests, and can even be used as a very fast screening tool to assess susceptibility to hearing damage caused by noise before entering high-end industrial environments. And considered sound.

Determining the Pattern of Physical Activity of Children in Markazi and Isfahan Provinces in 2002

Fatemeh Dareh

Introduction: Among the risk factors for coronary artery disease, less attention has been paid to physical activity, especially in children and adolescents. Various studies have shown that their level of physical activity is lower than the standard for maintaining lower health. In this study, the pattern of physical activity of children and adolescents in the central regions of Iran is examined.
Method: In this descriptive study, which is part of the first phase of the Isfahan Healthy Heart Project and was conducted in 2002, awareness of the attitude and performance of 4,000 urban and rural children and adolescents in preschool to high school in Isfahan (Isfahan, Najafabad) and Markazi (Arak city) and the parents of these students and 500 teachers and school officials have been examined about physical activity and the duration of watching TV through a questionnaire. Sampling was selected in the form of a random cluster and in proportion to the urban and rural population living in the two provinces. Data were analyzed with SPSS software and p<0.05 were considered significant.
Results: About 40 percent of middle and high school students in the two provinces practiced regular sports at school less than two hours a week. Regular morning exercise and school sports were more common in girls than boys (p<0.00001), but regular extracurricular exercise and sports team membership were more common in boys (P<0.001). More than 77 percent of parents were unaware of the importance of childhood exercise in their children's health. The average TV viewing time for children of different ages was 4 hours a day.
Conclusion: The level of physical activity of middle and high school students in the central regions of Iran is less than the desired level and the duration of watching TV is more than the recommended values.

Investigating the Relationship Between Psoriasis and the Presence of Candida Albicans in the Oral Cavity

Abbas Rezaei

Introduction: Some articles have suggested an increase in the prevalence of oral candidiasis in patients with psoriasis, as well as the potential for exacerbation or chronicity of psoriasis. Therefore, the prevalence of oral candidiasis in patients with psoriasis and its comparison with healthy individuals has been considered.
method: In this cross-sectional study, 43 people with psoriasis and 43 seemingly healthy individuals referred to dermatology clinics affiliated with Isfahan University of Medical Sciences were randomly selected and examined for the presence or absence of Candida albicans in the oral mucosa by direct lam and culture. Were placed.
Results: According to the results of this analytical study, 44.2% of patients with psoriasis with Candida albicans were infected with oral cavity, which was 25.6% for healthy individuals, and this difference was significant (p=0.05). There was also a significant relationship between the severity of psoriasis and the prevalence of oral Candida albicans (p=0.05).
Conclusion: The study found that candida yeast was more common in patients with psoriasis than in healthy people. According to the results of this study, it is recommended that all patients with psoriasis be examined for the presence of Candida albicans in the mouth and, if the test is positive, be treated with antifungal drugs.

Abdoljalal Marjani

Serological and Molecular Evidence of Hepatitis B in Patients With Chronic Hepatitis C

Zahra Honarkar

Introduction: Hidden hepatitis B is a condition in which the surface antigen of hepatitis B in the patient's serum is negative but the DNA of the hepatitis B virus is detected in the serum or liver tissue. In this study, the prevalence of latent hepatitis B in chronic hepatitis C patients and their biochemical and histological changes were investigated.
method: In this descriptive study, target sampling was performed so that 27 chronic hepatitis C patients whose HBsAg was negative and during 2001 and 2002 to two hepatitis centers of Tehran and Research Center for Gastrointestinal and Liver Diseases of Shahid University of Medical Sciences Beheshti came in and underwent liver sampling. On the hepatic paraffinic block of these patients, polymerase chain reaction tests were performed for the presence of HBVDNA, as well as immunohistochemical tests for the presence and detection of HBsAg and central hepatitis B antigen.
Results: Of the 27 PCR samples examined, patients reported positive HBVDNA in 5 cases (19%). In all of these patients, IHC tests were reported to be negative for HBsAg and HBcAg. Histological changes of cirrhosis and irreversible cirrhosis symptoms were seen only in the HBVDNA group.
Conclusion: The prevalence of latent hepatitis B is relatively high in patients with hepatitis C. In these patients, latent hepatitis B can exacerbate liver damage and accelerate the progression of cirrhosis.

Comparison of the Therapeutic Effect and Side Effects of Sodium Propofol and Thiopental in Patients Treated With Electroconvulsive Therapy

Bijan Yazdi

Introduction: Electric seizure therapy is one of the most important treatments in psychiatry that is used as the least effective treatment for mental disorders. Significant anesthetics commonly used in Iran for this purpose are sodium thiopental with a dose of 0.5-3.5 mg/kg and propofol with a dose of 2-3 mg/kg, each of which has the above effects. It focuses on different systems of the body and causes different autonomic responses in patients. Therefore, it is necessary to compare the therapeutic effects and side effects of these two drugs in patients undergoing treatment for seizures with electricity.
method: In this study, which is a cross-sectional clinical trial study, 40 patients in the field of electronics seizure therapy were examined twice, one time with propofol and the next with sodium thiopental to induce anesthesia. Pre-, post-operative, and postoperative information were collected and analyzed by a questionnaire for seizure duration, blood pressure, heart rate, and level of awareness.
Results: The duration of seizures in propofol was less than in thiopental, which was statistically significant (P<0.001). Blood fluid in the case of thiopental increased significantly compared to propofol (P<0.001). The thiopental receptor group also showed a higher increase in heart rate, but this difference was not significant.
Conclusion: The use of propofol in seismic therapy with electricity has fewer side effects than thiopental and its use is recommended.