Volume 28, Issue 1 (3-2025)
J Arak Uni Med Sci 2025, 28(1): 68-77 |
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Khalvati S, Foroutan T, Momeni-Moghaddam M, Hajjar T. Morphometric and Histological Study of the Kidney of Offspring of Diabetic Mothers Treated with Insulin. J Arak Uni Med Sci 2025; 28 (1) :68-77
URL: http://jams.arakmu.ac.ir/article-1-7785-en.html
URL: http://jams.arakmu.ac.ir/article-1-7785-en.html
1- Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
2- Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran ,tataforoutan@gmail.com
3- Department of Biology, Hakim Sabzevari University, Sabzevar, Iran
2- Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran ,
3- Department of Biology, Hakim Sabzevari University, Sabzevar, Iran
Abstract: (237 Views)
Introduction: This study aimed to investigate the effects of insulin drug therapy on the kidney structure of offspring of insulin-treated diabetic mice.
Methods: After pregnancy, female Sprague-Dawley rats were divided into control, sham, diabetics induced by streptozotocin, and diabetics receiving insulin groups. Diabetic rat offspring were sacrificed on the 10th day after natural childbirth, and the left kidneys were studied for morphometric and histological studies.
Results: The weight of children of diabetic mothers showed a significant decrease compared to children of healthy mothers (P < 0.001). The weight of the offspring of diabetic mothers receiving insulin did not show a substantial change compared to the offspring of diabetic mothers. The weight of the kidneys of the offspring of diabetic mothers showed a significant decrease compared to the control group. However, the weight of the kidneys of the offspring of diabetic mothers receiving insulin did not increase compared to those of diabetic mothers. The results of the kidney sections stained with hematoxylin-eosin and trichrome Masson showed that the offspring of diabetic mothers treated with insulin did not show a significant improvement compared to the control group.
Conclusions: Although the use of insulin is one of the momentous therapeutic ways to control blood glucose in diabetics, it cannot significantly lead to the normal health of the kidneys of the offspring of diabetic mothers under insulin treatment.
Methods: After pregnancy, female Sprague-Dawley rats were divided into control, sham, diabetics induced by streptozotocin, and diabetics receiving insulin groups. Diabetic rat offspring were sacrificed on the 10th day after natural childbirth, and the left kidneys were studied for morphometric and histological studies.
Results: The weight of children of diabetic mothers showed a significant decrease compared to children of healthy mothers (P < 0.001). The weight of the offspring of diabetic mothers receiving insulin did not show a substantial change compared to the offspring of diabetic mothers. The weight of the kidneys of the offspring of diabetic mothers showed a significant decrease compared to the control group. However, the weight of the kidneys of the offspring of diabetic mothers receiving insulin did not increase compared to those of diabetic mothers. The results of the kidney sections stained with hematoxylin-eosin and trichrome Masson showed that the offspring of diabetic mothers treated with insulin did not show a significant improvement compared to the control group.
Conclusions: Although the use of insulin is one of the momentous therapeutic ways to control blood glucose in diabetics, it cannot significantly lead to the normal health of the kidneys of the offspring of diabetic mothers under insulin treatment.
References
1. Katsarou A, Gudbjörnsdottir S, Rawshani A, Dabelea D, Bonifacio E, Anderson BJ, et al. Type 1 diabetes mellitus. Nat Rev Dis Primers. 2017;3:17016. pmid: 28358037 doi: 10.1038/nrdp.2017.16.
2. Mohammadi P. Teratogenic effects of some factors on the human fetus: a review study [in Persian]. J Mil Med. 2017;19(5):423-31.
3. Nold JL, Georgieff MK. Infants of diabetic mothers. Pediatr Clin North Am. 2004;51(3):619-37. pmid: 15157588 doi: 10.1016/j.pcl.2004.01.003
4. Amri K, Freund N, Vilar J, Merlet-Benichou C, Lelievre-Pegorier M. Adverse effects of hyperglycemia on kidney development in rats: in vivo and in vitro studies. Diabetes. 1999;48(11):2240-5. pmid: 10535460 doi: 10.2337/diabetes.48.11.2240
5. Parimi M, Nitsch D. A systematic review and meta-analysis of diabetes during pregnancy and congenital genitourinary abnormalities. Kidney Int Rep. 2020;5(5):678-93. pmid: 32405589 doi: 10.1016/j.ekir.2020.02.1027
6. Zabihi S, Loeken MR. Understanding diabetic teratogenesis: where are we now and where are we going? Birth Defects Res A Clin Mol Teratol. 2010;88(10):779-90. pmid: 20706996 doi: 10.1002/bdra.20704
7. Rocha SO, Gomes GN, Forti ALL, do Carmo Pinho Franco M, Fortes ZB, de Fátima Cavanal M, et al. Long-term effects of maternal diabetes on vascular reactivity and renal function in rat male offspring. Pediatr Res. 2005;58(6):1274-9. pmid: 16306207 doi: 10.1203/01.pdr.0000188698.58021.ff.
8. Nkonge KM, Nkonge DK, Nkonge TN. Insulin therapy for the management of diabetes mellitus: a narrative review of innovative treatment strategies. Diabetes Ther. 2023;14(11):1801-31. pmid: 37736787 doi: 10.1007/s13300-023-01468-4
9. Battin M, Wouldes TA, Rowan J. Neurodevelopmental outcome in offspring born following gestational diabetes. In: Rajendram R, Preedy V, Patel V. editor. Nutrition and Diet in Maternal Diabetes. Humana Press, Cham: Nutrition and Health; 2018. doi: 10.1007/978-3-319-56440-1_27
10. França-Silva N, Oliveira NDG, Balbi APC. Morphofunctional renal alterations in rats induced by intrauterine hyperglycemic environment. Arch Med Sci. 2016;12(2):243-51. pmid: 27186167 doi: 10.5114/aoms.2015.48220
11. Miner JH. Type IV collagen and diabetic kidney disease. Nat Rev Nephrol. 2020; 16:3–4. doi:10.1038/s41581-019-0229-1
12. Miodovnik M, Lavin JP, Knowles HC, Holroyde J, Stys SJ. Spontaneous abortion among insulin-dependent diabetic women. Am J Obstet Gynecol. 1984;150(4):372-6. pmid: 6207729 doi:10.1016/S0002-9378(84)80141-6
13. Neves HM, Sgarbosa F, Calderon IMP, Vianna LS, Santini ACM, Sieiro Netto L, et al. Does hyperglycemia in pregnancy change fetal kidney growth? a longitudinal prospective study. Rev Bras Ginecol Obstet. 2013;35:442-6. pmid: 24337055 doi:10.1590/S0100-72032013001000003
14. Pedersen J. Diabetes and pregnancy; blood sugar of newborn infants during fasting and glucose administration. Ugeskr Laeger. 2014;114(21):685. pmid: 14958933
15. Arshad R, Karim N, Hasan JA. Effects of insulin on placental, fetal and maternal outcomes in gestational diabetes mellitus. Pak J Med Sci. 2014;30(2):240-4. pmid: 24772119 doi:10.12669/pjms.302.4396
16. Dong MZ, Li QN, Fan LH, Li L, Shen W, Wang ZB, et al. Diabetic uterine environment leads to disorders in metabolism of offspring. Front Cell Dev Biol. 2021;9:706879. pmid: 34381787 doi: 10.3389/fcell.2021.706879
17. Gutaj P, Wender-Ozegowska E. Diagnosis and management of IUGR in pregnancy complicated by type 1 diabetes mellitus. Curr Diab Rep. 2016;16(5):39. pmid: 26983627 doi:10.1007/s11892-016-0732-8
18. Langmia IM, Kräker K, Weiss SE, Haase N, Schütte T, Herse F, et al. Cardiovascular programming during and after diabetic pregnancy: role of placental dysfunction and IUGR. Front Endocrinol (Lausanne). 2019;10:215. pmid: 31024453 doi: 10.3389/fendo.2019.00215
19. Kong L, Nilsson I A, Gissler M, Lavebratt C. Associations of maternal diabetes and body mass index with offspring birth weight and prematurity. JAMA Pediatr. 2019;173(4):371-8. pmid: 30801637 doi:10.1001/jamapediatrics.2018.5541
20. Ojeda NB, Grigore D, Alexander BT. Intrauterine growth restriction: fetal programming of hypertension and kidney disease. Advances in chronic kidney disease. 2008;15(2):101-6. doi: 10.1053/j.ackd.2008.01.001
21. 21.Bhandari J, Thada RK, Khattar D. Diabetic Embryopathy. Treasure Island (FL): StatPearls Publishing; 2025.
22. Dudley DJ. Diabetic-associated stillbirth: incidence, pathophysiology, and prevention. Obstet Gynecol Clin North Am. 2007;34(4):293-307. pmid: 17572273 doi: 10.1016/j.ogc.2007.03.001
23. Liu Y, Chen H, Li H, Li L, Wu J, Li H. Effect and safety of adding metformin to insulin therapy in treating adolescents with type 1 diabetes Mellitus: an updated meta-analysis of 10 randomized controlled trials. Front Endocrinol (Lausanne). 2022;13:878585. pmid: 35707462 doi:10.3389/fendo.2022.878585
24. Kular JK, Basu S, Sharma RI. The extracellular matrix: Structure, composition, age-related differences, tools for analysis and applications for tissue engineering. J Tissue Eng. 2014;5:2041731414557112. doi: 10. 2041731414557112.
25. Ruiz-Palacios M, Ruiz-Alcaraz AJ, Sanchez-Campillo M, Larqué E. Role of insulin in placental transport of nutrients in gestational diabetes mellitus. Ann Nutr Metab. 2017;70(1):16-25. pmid: 28110332 doi:10.1159/000455904.
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