Volume 21, Issue 4 (8-2018)
J Arak Uni Med Sci 2018, 21(4): 98-108 |
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Najafi M, Asad M R, Rahimi M, Souri R, Ahvar A. The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male Rats. J Arak Uni Med Sci 2018; 21 (4) :98-108
URL: http://jams.arakmu.ac.ir/article-1-5540-en.html
URL: http://jams.arakmu.ac.ir/article-1-5540-en.html
1- Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- Department of Exercise Physiology, University of Payam Noor, Tehran, Iran.
3- Department of Physical Education and Sport Sciences, Faculty of Humanities, Shahrekord University, Shahrekord, Iran. ,mostafa.rahimi20@gmail.com
4- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
2- Department of Exercise Physiology, University of Payam Noor, Tehran, Iran.
3- Department of Physical Education and Sport Sciences, Faculty of Humanities, Shahrekord University, Shahrekord, Iran. ,
4- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
Abstract: (3606 Views)
| Background and Aim: The aim of study was to investigate the effect of 8 weeks of moderate-intensity continuous training (MICT) and high intensity interval training (HIIT) on the GLUT4 gene expression in skeletal muscle of obese male rats. Materials and Methods: Twenty-eight male Wistar rats with 150 ± 25 grams body weight were divided into base control (n = 6), obese control (n = 6), MICT (n = 8), and (n = 8) HIIT groups as study sample. At the beginning of the study, base control group rats were sacrificed. In other groups, rats underwent high fat regime diet for 8 weeks. After one week of familiarization, rats in training groups run on treadmill for 8 weeks and 5 days per week. Forty-eight hours after the last session, the rats were scarified and gastrocnemius muscle tissue sample were removed. GLUT4 gene expression was measured by Real-Time PCR methods. Findings: Results indicated that high fat diet for 8 weeks significantly increased rats body weight (p= 0.001), whereas both of MICT and HIIT training significantly decreased body weight (p= 0.001). Moreover, the MICT and HIIT had significant increase in GLUT4 gene expression in gastrocnemius muscle of obese rats (p = 0.001). Although, there were no differences between two experimental groups (p = 0.99). Conclusion: According to the results, the MICT and HIIT increase gene expression of GLUT4 in mixed muscle fibers in obese rats. |
Keywords: Glucose transporter 4, Moderate-intensity continuous training, High intensity interval training, Skeletal muscle
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