Volume 21, Issue 1 (4-2018)
J Arak Uni Med Sci 2018, 21(1): 40-51 |
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Salem M, Bayrami A, Mirzapour T, Sagha M. Evaluation of the Effects of Different Concentrations All-trans Retinoic Acid on the Survival of Bone Marrow Mesenchymal Stem Cells. J Arak Uni Med Sci 2018; 21 (1) :40-51
URL: http://jams.arakmu.ac.ir/article-1-5514-en.html
URL: http://jams.arakmu.ac.ir/article-1-5514-en.html
1- MOHAGEG ARDEBIL UNIVERSITY
2- DEBIL UNIVERSITY ,abolfazlbayrami@gmail.com
3- DEBIL UNIVERSITY
4- Ardabil University of Medical Sciences
2- DEBIL UNIVERSITY ,
3- DEBIL UNIVERSITY
4- Ardabil University of Medical Sciences
Abstract: (3486 Views)
Abstract
Background: According to application of Retinoic acid in differentiation of the stem cells to different cells and its role in apoptotic of cancer cells, the selection of appropriate dose for differentiation of stem cells is important. Thus in this study the effects of Retinoic acid in different concentrations on viability stem cells to select the appropriate dose for differentiation was investigated.
Materials and Methods: In this study, bone marrow mesenchymal stem cells were affected by different concentrations of Retinoic acid. Survival of cells was investigated after 3, 10 and 15 days of culture by MTT assay. DAPI staining was used to evaluate the number of apoplectic nuclei in treated cells after 10 and 15 days.
Results: After three days of culture, the results showed that a large number of cells are destroyed at concentrations of 10-4, 10-3 and 10-2M of Retinoic acid, while in 10-5 and 10-6 M of Retinoic acid, it is not observed many apoptosis. Amount of 10-5M Retinoic acid after 10 days showed significant apoptosis, while the concentration of 10-6 M Retinoic acid after 15 days showed significant apoptosis compared to the control group (p<0.05).
Conclusion: It looks that 10-6 M Retinoic acid is an appropriate concentration for differentiation of mesenchymal stem cells.
Background: According to application of Retinoic acid in differentiation of the stem cells to different cells and its role in apoptotic of cancer cells, the selection of appropriate dose for differentiation of stem cells is important. Thus in this study the effects of Retinoic acid in different concentrations on viability stem cells to select the appropriate dose for differentiation was investigated.
Materials and Methods: In this study, bone marrow mesenchymal stem cells were affected by different concentrations of Retinoic acid. Survival of cells was investigated after 3, 10 and 15 days of culture by MTT assay. DAPI staining was used to evaluate the number of apoplectic nuclei in treated cells after 10 and 15 days.
Results: After three days of culture, the results showed that a large number of cells are destroyed at concentrations of 10-4, 10-3 and 10-2M of Retinoic acid, while in 10-5 and 10-6 M of Retinoic acid, it is not observed many apoptosis. Amount of 10-5M Retinoic acid after 10 days showed significant apoptosis, while the concentration of 10-6 M Retinoic acid after 15 days showed significant apoptosis compared to the control group (p<0.05).
Conclusion: It looks that 10-6 M Retinoic acid is an appropriate concentration for differentiation of mesenchymal stem cells.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2017/12/16 | Accepted: 2018/02/26
Received: 2017/12/16 | Accepted: 2018/02/26
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