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:: Volume 15, Number 1 (April-May 2012) ::
amuj 2012, 15(1): 1-9 Back to browse issues page
Phenotypic and molecular detection of TEM, PER, and VEB beta-lactamases in clinical strains of Escherichia coli
Majid Eslami, Shahin Najar Peerayeh
Associate professor , najarp_s@modares.ac.ir
Abstract:   (7580 Views)
Background: TEM, PER, and VEB are extended-spectrum betalactamase (ESBL) enzymes that are capable of hydrolyzing penicillins, cephalosporins, and aztreonam. The aim of this study was to determine the prevalence of ESBL producing E.coli and molecular evaluation of TEM, PER, and VEB β-lactamases among E.coli strains. Materials and Methods: A total of 200 clinical strains of E.coli were isolated from clinical specimens and their antibiotic susceptibility was determined by disk diffusion method. ESBL production was determined using the combined disk method with CAZ and CTX with clavulanic acid and alone. Minimum concentration inhibition (MIC) for CAZ and CTX with clavulanic acid and alone was determined by agar dilution method. Finally, PCR with specific primers was used for determining the presence of blaTEM, blaPER, and blaVEB genes. Results: Combined disk method confirmed 94 strains (47%) to be ESBL producing E.coli. Of the 94 ESBL producing strains, 36 samples had MIC=16, 44 samples had MIC between 32-256, and 10 samples had MIC≥512 for ceftazidime, whereas 8 samples had MIC=16, 68 samples had MIC between 32-256, and 21 samples had MIC≥512 for cefotaxime. The frequency of TEM was 44% however, blaPER and blaVEB genes were not detected by PCR among ESBL producing isolates. Conclusion:The results indicated that the high percentage of ESBL producing E.coli is 47% and PCR method showed a high frequency of TEM enzyme, but PER and VEB betalactamase were not found among them.
Keywords: Beta-lactamase, Combined disk, ESBL, Escherichia coli
Full-Text [PDF 380 kb]   (2617 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2010/12/25 | Accepted: 2011/05/9
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Eslami M, Najar Peerayeh S. Phenotypic and molecular detection of TEM, PER, and VEB beta-lactamases in clinical strains of Escherichia coli. amuj. 2012; 15 (1) :1-9
URL: http://amuj.arakmu.ac.ir/article-1-969-en.html
Volume 15, Number 1 (April-May 2012) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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