[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 13, Number 2 (6-2010) ::
amuj 2010, 13(2): 55-62 Back to browse issues page
The effect of short time exposure to 4°C temperature on the expression profiles of mono-carboxylic transporter genes 1, 2, 3, and 4 in 4-cell mouse embryos
Mina Ramezani , Ahmad Hosseini, Bahram kazemi, Arghavan Janan
َAssistant professor , mina.ramezani@gmail.com
Abstract:   (8501 Views)
Background: One way of embryo preservation is cryopreservation, but this process may damage and lead to the loss of the embryos, and bring about chromosomal abnormality. This has led researchers to seek techniques for short term preservation of embryos in 0-10 ºC temperatures. The aim of this study was to determine the effect of short time exposure to 4°C temperature on the expression profiles of mono-carboxylic transporter genes 1,2 ,3, and 4(MCT1-4) in 4-cell mouse embryos. Materials and Methods: In this fundamental study, forty 4-cell mouse embryos from NMRI strain were randomly divided into two groups. The first group consisted of fresh 4-cell embryos, and the second group included 4-cell mouse embryos that were exposed to 4°C temperature for 24 hours. After RT-PCR, the samples were electro-phoresised for expressing the MTC1-4 genes. Results: The expression of MCT 1-3 was observed in the first group, but the obtained results did not indicate their expression in the second group. Conclusion: Preservation of 4-cell embryos in 4°C for 24 hours inhibits the expression of MCT 1-3 genes. Keeping embryos in 4°C temperature is not a proper way for their short time preservation.
Keywords: Four-cell embryo, Mice, Mono-carboxylic transporter genes, PCR
Full-Text [PDF 287 kb]   (1571 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2009/12/18
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ramezani M, Hosseini A, kazemi B, Janan A. The effect of short time exposure to 4°C temperature on the expression profiles of mono-carboxylic transporter genes 1, 2, 3, and 4 in 4-cell mouse embryos. amuj. 2010; 13 (2) :55-62
URL: http://amuj.arakmu.ac.ir/article-1-562-en.html
Volume 13, Number 2 (6-2010) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
Persian site map - English site map - Created in 0.055 seconds with 793 queries by yektaweb 3506