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:: Volume 20, Number 7 (10-2017) ::
amuj 2017, 20(7): 65-76 Back to browse issues page
Effects of Ethanol Preconditioning on Pentylenetetrazole-induced Memory Impairment and Expression of NMDA Receptor NR1 Subunit mRNA in Rat
Azam Alinaghipour, Marziyeh Tavassoli, Elahe Seyed Hosseini, Abolfazl Ardjmand
Associate Professor, Ph.D of Neuroscience, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran , ardjmand_ab@kaums.ac.ir
Abstract:   (348 Views)
Background: Neuronal damage following seizures and epilepsy is one of the main causes of disabilities and mortality worldwide. In recent years, preconditioning has been introduced as a novel strategy for the prevention of brain damage. Preconditioning is a phenomenon in which a minor noxious stimulus protects from a subsequent more severe insult. The aim of present study was to examine the effect of ethanol (Eth) preconditioning on pentylenetetrazole (PTZ)-induced impairment memory in the inhibitory avoidance model.
Material and Methods: This study was carried out on 45 adult male Wistar rats (180-200 g). Animals were assigned into five groups: Control, Eth 0.25, Eth 0.5, PTZ and Eth (0.5) +PTZ (n=9, for all groups). Eth-preconditioning was induced 6 days before the injection of PTZ. The animals were tested in a single trial step-through inhibitory test in two sessions (train and test). Then locomotor activity of rats was recorded in the open-field apparatus and NR1 mRNA expression in the hippocampus was measured by real-time PCR technique.
Results: One-way ANOVA revealed that the Ethanol preconditioning did not impair inhibitory memory. Further, post-test analyses showed that Ethanol preconditioning significantly prevented from PTZ-induced memory impairment, and increased NR1 subunit mRNA expression in PTZ-induced memory impairment group. In addition, one-way ANOVA for the locomotor activity showed no significant difference between the groups.
Conclusion: Our results showed that a pre-conditioning treatment with Ethanol
(0.5g/kg/day), 6 days before PTZ-induced memory impairment may provide a kind of neuroprotection in rats.

Keywords: Epilepsy, Ethanol, Inhibitory avoidance Memory, Preconditioning, Pentylenetetrazole, Seizure
Full-Text [PDF 1063 kb]   (107 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2017/07/15 | Accepted: 2017/08/2 | Published: 2017/09/13
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Alinaghipour A, Tavassoli M, Seyed Hosseini E, Ardjmand A. Effects of Ethanol Preconditioning on Pentylenetetrazole-induced Memory Impairment and Expression of NMDA Receptor NR1 Subunit mRNA in Rat. amuj. 2017; 20 (7) :65-76
URL: http://amuj.arakmu.ac.ir/article-1-5225-en.html
Volume 20, Number 7 (10-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی اراک Arak Medical University Journal
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